The effect of antiallergic intranasal formulations on ciliary beat frequency of human nasal epithelium in vitro

Abstract

The nasal mucociliary clearance is an important defense mechanism of the upper respiratory tract. It is known to be influenced by many pharmacologic substances. We investigated the effects of three topical intranasal antiallergic formulations containing disodium cromoglycate (DNCG), dimethindene maleate (DMM), and azelastine-HCL (AZL) on nasal ciliary beat frequency (CBF) in vitro. Nasal ciliated cells were harvested from 16 healthy volunteers. Cells were diluted 1:10 in culture medium and incubated with a placebo formulation (PLAC), containing 0.022% benzalkonium chloride, which was part of all formulations, a registered 2% formulation of DNCG, a 0.1% formulation of DMM, and a registered 0.1% formulation of AZL. After an incubation period of 20 min, CBF was registered by a photoelectric measurement device. Under control conditions (CONTROL), CBF was 11.4 +/- 1.3 Hz. PLAC reduced CBF to 9.7 +/- 2.3 Hz (NS). DNCG reduced CBF to 9.7 +/- 2 Hz (NS). DMM reduced CBF to 7.2 +/- 1.7 Hz (P < or = 0.05 vs CONTROL, NS vs PLAC), and AZL reduced CBF to 0.9 +/- 1.8 Hz (P < or = 0.001 vs CONTROL, P < or = 0.001 vs DNCG, P < or = 0.001 vs PLAC). In conclusion, a possible influence of antiallergic intranasal formulations on nasal ciliary function has to be considered in clinical application.

Apnea and nasal mucociliary clearance

“…[14,15] Bu sistem akut ya da kronik rinit veya nazal alerjiler gibi patolojik koşullardan; yaşlanma ve sıcaklık (<10 °C ve >45 °C) gibi çeşitli faktörlerden; adrenalin, asetilkolin ve kortikosteroidler gibi farmakolojik ajanlardan etkilenir. [12,16,17] Nazal septum deviyasyonu, alerjik rinit, polip ve sinüziti olan hastalarda burnun mukosiliyer fonksiyonun bozulmuş olduğu gösterildiğinden bu patolojilerden herhangi biri tespit edilen hastalar çalışmaya alınmadı. [10,11,13,18,19] Burun mukozasının siliyer aktivitesini değerlendirmek pek çok araştırmacının hedefi olmuştur.…”

Allergic rhinitis: Selective comparisons of the pharmaceutical options for management

“…8,9 But there are adequate studies to suggest that topically applied azelastine actually may assist in repairing the damage associated with sulfur dioxide and improve ciliary beat frequency. The speed in which topical antihistamines work, in some cases within 5 minutes, makes them most attractive 10,11 and may lead some patients to use a strategy of erratic treatment with success.…”

Treatment of respiratory diseases with inhalation patches

“…Учитывая возрастающую активность использования интраназальных препаратов как во взрослый практике, так и в педиатрической, ученые начали исследовать воздействие различных фармакологических веществ на мерцательный эпителий. Так, в литературе описана степень угнетения работы мерцательного эпителия СО полости носа после воздействия различных лекарственных препаратов: интраназальных противоаллергических [5], деконгестантов [6,7], антимикробных препаратов [8,9], анестетиков [10], топических глюкокортикостероидов [6]. Следует также отметить, что угнетение мукоцилиарного транспорта зависит от концентрации действующего вещества [11] и от содержания дополнительных компонентов, в частности консервантов [7].…”