The effect of anthracene-based chalcone derivatives in the resazurin dye reduction assay mechanisms for the investigation of Gram-positive and Gram-negative bacterial and fungal infection

Prakash, Gopi; Boopathy, Mani; Selvam, Rajiv; Kumar, Sushil; Subramanian, K.

doi:10.1039/c7nj04125j

Cited by 18 publications

(11 citation statements)

References 41 publications

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“…Therefore, wells that appear purple or blue are indicative of the absence or loss of viability of the bacteria, while the pink wells indicate cell viability despite the presence of bioactive compounds. 43,44 Four representative plates of this experiment are shown in Figure S2, one for each strain. The first line of wells A1−A6 represent culture medium and solvent controls: where the first three wells have only BHI culture medium, noticing the blue/ purple color of the dye, and subsequently in all cases the three pink wells correspond to the culture medium with the respective bacterial inoculum, and the remaining 6 wells (A7−A12) contain DMSO as a negative control.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Tetrandrine Derivatives as Promising Antibacterial Agents

et al. 2023

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This work reports on the antibacterial activity of two tetrandrine derivatives, with acridine (MAcT) and anthracene (MAnT) units, against Gram-positive and Gram-negative bacteria of clinical importance by the broth microdilution method as well as their antioxidant activity against ABTS •+ and DPPH •+ radicals. Unlike natural tetrandrine, its derivatives inhibited bacterial growth, showing selectivity against Staphylococcus aureus with notable activity of MAnT (MIC = 0.035 μg/mL); this compound also has good activity against the ABTS •+ radical (IC 50 = 4.59 μg/mL). Cell membrane integrity studies and reactive oxygen species (ROS) detection by fluorescent stains helped to understand possible mechanisms related to antibacterial activity, while electrophoretic mobility assays showed that the derivatives can bind to bacterial DNA plasmid. The results indicate that MAnT can induce a general state of oxidative stress in S. aureus and Escherichia coli, while MAcT induces an oxidative response in S. aureus. Complementary electrochemical studies were included.

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Section: ■ Results and Discussionmentioning

confidence: 99%

“…Plates were incubated at 37 °C for 24 h. The OD 600 values for each well were determined with a Multiskan microplate reader (Thermo Scientific, Inc. Multiskan GO, NY). Then, 15 μL per well of the resazurin dye (0.02%) was added and the microplate was incubated for 4 h at 37 °C, and the OD 600 values were determined again. , …”

Section: Methodsmentioning

confidence: 99%

Tetrandrine Derivatives as Promising Antibacterial Agents

et al. 2023

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“…The hybrid ANID ( 4 ; MIC: 3.12–25 μM) showed promising antibacterial ( S. aureus , B. cereus , S. faecalis , E. coli , K. pneumonia , and Salmonella typhimurium ) and antifungal ( Candida albicans , Candida glabrata , and Candida cruzi ) activity, and the antibacterial activity (MIC: 3.12–6.25 μM) was at the same level with that of streptomycin (MIC: 1.56–3.12 μM) against S. aureus , K. pneumonia , and S. typhimurium . [ 29 ] Replacement of anthracene moiety by ferrocene motif or the introduction of 3‐amino‐2‐hydroxypropyl at the N‐1 position of indole moiety was detrimental to the activity, as evidenced by the fact that hybrid 5 (MIC: 125 and 250 μM) only showed weak activity against P. aeruginosa and B. subtilis . [ 30,31 ]…”

Section: Indole–chalcone Hybridsmentioning

confidence: 99%

Indole/isatin‐containing hybrids as potential antibacterial agents

Song

Bian

et al. 2020

Archiv der Pharmazie

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The emergence and worldwide spread of drug-resistant bacteria have already posed a serious threat to human life, creating the urgent need to develop potent and novel antibacterial drug candidates with high efficacy. Indole and isatin (indole-2,3-dione) present a wide structural and mechanistic diversity, so their derivatives possess various pharmacological properties and occupy a salient place in the development of new drugs. Indole/isatin-containing hybrids, which demonstrate a promising activity against a panel of clinically important Gram-positive and Gram-negative bacteria, are privileged scaffolds for the discovery of novel antibacterial candidates. This review, covering articles published between January 2015 and May 2020, focuses on the development and structure-activity relationship (SAR) of indole/isatincontaining hybrids with potential application for fighting bacterial infections, to facilitate further rational design of novel drug candidates.

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“…Indicator bacteria: E. coli, S. aureus, A. niger. Determined by the 96-well microdilution method (resazurin as indicator) [28]. The microbial cultures of the pathogens were maintained on slants of appropriate media like the beef extract peptone medium for bacteria and the PDA solid medium for the fungal species.…”

Section: Minimum Inhibitory Concentration (Mic) Determinationmentioning

confidence: 99%

Kaempferol separated from Camellia oleifera meal by high-speed countercurrent chromatography for antibacterial application

Qiu

Yang

et al. 2020

Eur Food Res Technol

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Natural biologically active substances have received continuous attention for the potentially beneficial health properties against chronic diseases. In this study, bacteriostatic active substance from Camellia oleifera meal, which is a major byproduct of the Camellia oil processing industry, were extracted with continuous phase change extraction (CPCE) method and separated by HSCCC. Compared with traditional extraction methods, CPCE possessed higher extraction efficiency. Two main substances were separated and purified (above 90.0%). The structure of them were further identified by UV, LC-ESI-MS-MS, 1H-NMR, and 13C-NMR as flavonoids F2 kaempferol 3-O-[β-d-glucopyranosyl-(1 → 2)-α-l-rhamnopyranosyl-(1 → 6)]-β-dglucopyranoside and J2 kaempferol 3-O-[β-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranosyl-(1 → 6)]-β-d-glucopyranoside for the first time in C. Oleifera meal. The results of antibacterial activity measurement showed that both compounds have excellent antibacterial activity. And the antibacterial stability of F2 were finally confirmed: F2 showed broad spectrum antibacterial activity against Escherichia coli, Staphylococcus aureus, Salmonella enteriditis, Bacillus thuringiensis, Aspergillus niger and Rhizopus nigricans. Besides, F2 exhibited relatively high stable property even at high temperature, acid and metal ion solutions. The findings of this work suggest the possibility of employing C. oleifera meal as an attractive source of health-promoting compounds, and at the same time facilitate its high-value reuse and reduction of environmental burden.

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The effect of anthracene-based chalcone derivatives in the resazurin dye reduction assay mechanisms for the investigation of Gram-positive and Gram-negative bacterial and fungal infection

Abstract: A novel series of anthracene-based chalcone derivatives were synthesized and evaluated in terms of their antibacterial and antifungal activities.

Cited by 18 publications

References 41 publications

Tetrandrine Derivatives as Promising Antibacterial Agents

Tetrandrine Derivatives as Promising Antibacterial Agents

Indole/isatin‐containing hybrids as potential antibacterial agents

Kaempferol separated from Camellia oleifera meal by high-speed countercurrent chromatography for antibacterial application

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