The effect of aluminium on bioelectrical activity of the Nitellopsis obtusa cell membrane after H+-ATPase inhibition

Kisnierienë, Vilma; Sakalauskas, Vidmantas

doi:10.2478/s11535-007-0009-y

Cited by 5 publications

(4 citation statements)

References 27 publications

(33 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We propose to measure the width of AP at 50 mV repolarisation from the AP peak (Kisnieriene et al 2016) for evaluation of the effect of nickel (a moderately toxic metal) on K + channels. In another investigation we found that the average duration of half amplitude was 4.9 AE 0.65 s in control conditions, 7.7 AE 0.72 s in DCCD and 17 AE 2.36 s after addition of aluminium (Kisnierienë and Sakalauskas 2007). Our results clearly show that Al modulates the repolarisation process by increasing its duration (Sevriukova et al 2014b).…”

Section: Action Potential Pattern As a Biomarker Of Bc Effects On Memsupporting

confidence: 64%

Electrical signalling in Nitellopsis obtusa: potential biomarkers of biologically active compounds

Kisnierienë

Lapeikaite

Pupkis

2018

Functional Plant Biol.

View full text Add to dashboard Cite

The Nitellopsis obtusa (N.A.Desvaux) J.Groves cell provides a model system for complex investigation of instantaneous effects of various biologically active compounds (BC) on the generation of plant bioelectrical signals in vivo. Experimental evidence using multiple electrical signals as biomarkers of the effects of BC (acetylcholine, asparagine, glutamate, nicotine, aluminium, nickel and cadmium ions) is provided. The effect of BC on membrane transport systems involved in the cell excitability were tested by current clamp, voltage clamp and patch clamp methods. Membrane potential (MP) alterations and action potential (AP) patterns in response to BC were shown to represent the cell state. High discretisation frequency allows precise, high time resolution analysis of real-time processes measuring changes in excitation threshold, AP amplitude and velocity of repolarisation values after application of BC indicating the effect on ion channels involved in AP generation. Application of voltage clamp revealed that changes in AP peak value were caused not only by increment in averaged maximum amplitude of the Clcurrent, but in prolonged Clchannels' opening time also. The cytoplasmic droplet can serve as a model system in which the effects of BC on single tonoplast ion channel can be studied by patch clamping. Investigation of electrical cell-to-cell communication revealed evidence on the electrical signal transduction through plasmodesmata.

show abstract

Section: Action Potential Pattern As a Biomarker Of Bc Effects On Memsupporting

confidence: 64%

Electrical signalling in Nitellopsis obtusa: potential biomarkers of biologically active compounds

Kisnierienë

Lapeikaite

Pupkis

2018

Functional Plant Biol.

View full text Add to dashboard Cite

show abstract

“…The PM H + -ATPase activity can be inhibited by the specific inhibitor DCCD (Kisnierienë and Sakalauskas 2007). In this research, DCCD was used to identify the function of PM H + -ATPase in regulating rhizosphere pH.…”

Section: Resultsmentioning

confidence: 99%

Rhizosphere pH difference regulated by plasma membrane H+-ATPase is related to differential Al tolerance of two wheat cultivars

Yang¹,

Wang²,

Geng³

et al. 2011

PLANT SOIL ENVIRON

View full text Add to dashboard Cite

Aluminum (Al)-tolerance of different cultivars shows considerable differences. Elevation of rhizosphere pH is an external Al-resistant mechanism of plants. To elucidate the correlation between Al tolerance and the capacity of plants to modify the rhizosphere pH at different Al-tolerant levels, a comparative study on the wheat (Triticum aestivum L.) cultivars ET8 (Al-tolerant) and ES8 (Al-sensitive) was performed. Rhizosphere pH of ET8 was much higher than that of ES8 under the same treatment, significant correlations were obtained among all the data of rhizosphere pH and relative root elongation (R2 = 0.9209**), or Al content in root apex (R2 = 0.9321**), which indicated that Al tolerance may be related to pH changes in the rhizosphere. The elevation of rhizosphere pH was inhibited by H+-ATPase specific inhibitor DCCD (dicylcohexylcarbodiimide, 25 mmol). Relative PM (plasma membrane) H+-ATPase activity of ET8 was significantly higher than that of ES8 under the same treatment. Significant correlation between all the data of relative PM H+-ATPase activity and rhizosphere pH (R2 = 0.8319**) were obtained. Taken together, these results suggest that PM H+-ATPase was involved in regulating rhizosphere pH. Under Al stress, the Al-tolerant line showed a stronger capacity of up-regulating rhizosphere pH by PM H+-ATPase than the Al-sensitive line, which may explain the observed differences in Al tolerance between the two wheat cultivars.

show abstract

“…A microelectrode was inserted into the cell and electrical properties of the PM were measured post 1 h of insertion. Action potentials were elicited 6 times every five minutes in each solution ( Figure 1) by injecting depolarizing current (0.1 s duration 1 μA/ cm 2 square pulse) between two pools using Ag/AgCl wires [17].…”

Section: Methodsmentioning

confidence: 99%

The combined effect of Cd2+ and ACh on action potentials of Nitellopsis obtusa cells

et al. 2009

Self Cite

View full text Add to dashboard Cite

Abstract:Interrelations between the action of acetylcholine (ACh) and cadmium ions (Cd 2+ ) on bioelectrogenesis of Nitellopsis obtusa cells were investigated. We analyzed repetitively triggered action potentials (AP), their reproducibility, shape and dynamics of membrane potential after AP induction. ACh significantly increased membrane permeability only at high concentrations (1 mM and 5 mM). Repolarisation level of action potential after the first stimulus was much more positive in all cells treated with ACh as compared to the control. Differences of membrane potentials between points just before the first and the second stimuli were 23.4±.0 mV (control); 40.4±5.9 mV (1 mM ACh solution) and 57.7 ± 8.5 mV (5 mM ACh solution). Cd 2+ at 20 μM concentration was examined as a possible inhibitor of acetylcholinesterase (AChE) in vivo. We found that cadmium strengthens depolarizing effect of acetylcholine after the first stimulus. The highest velocity of AP repolarization was reduced after ACh application and Cd 2+ strengthened this effect. There were no differences in dynamics of membrane potential after repetitively triggered action potentials in ACh or ACh and Cd 2+ solutions. This shows that cadmium in small concentration acts as inhibitor of acetylcholinesterase.

show abstract

The effect of aluminium on bioelectrical activity of the Nitellopsis obtusa cell membrane after H+-ATPase inhibition

Cited by 5 publications

References 27 publications

Electrical signalling in Nitellopsis obtusa: potential biomarkers of biologically active compounds

Electrical signalling in Nitellopsis obtusa: potential biomarkers of biologically active compounds

Rhizosphere pH difference regulated by plasma membrane H<sup>+</sup>-ATPase is related to differential Al tolerance of two wheat cultivars

The combined effect of Cd2+ and ACh on action potentials of Nitellopsis obtusa cells

Contact Info

Product

Resources

About