The combined effect of Cd2+ and ACh on action potentials of Nitellopsis obtusa cells

Kisnierienë, Vilma; Sakalauskas, Vidmantas; Pleskaciauskas, Aleksandras; Yurin, Vladimir; Rukšėnas, Osvaldas

doi:10.2478/s11535-009-0028-y

Cited by 8 publications

(11 citation statements)

References 33 publications

(39 reference statements)

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“…Exposure of ACh to potassium K out or K in channels does not appear to exclude its action on the excitable chloride channels. We previously demonstrated [31] that the addition of ACh prolongs the repolarization of the action potential in 'pump state' cells just as Gong and Bisson have demonstrated in another Characeaen, Chara corallina [22]. They attributed this prolongation to the increased probability of the tonoplast chloride channels opening, which would tend to maintain cell in a more depolarized state.…”

Section: Discussionmentioning

confidence: 65%

“…This links ACh with numerous signalling processes in the so-called 'big network' of calcium signalling. In our previous work [31] we found that cadmium strengthens the depolarizing effect of acetylcholine on the repolarization phase of action potential and could be explained by an impact on the intracellular calcium level. The effect of ACh on the cytoplasmic Ca 2+ content requires a separate study.…”

Section: Discussionmentioning

confidence: 82%

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The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation

et al. 2012

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The role of acetylcholine (ACh) as a signalling molecule in plants was investigated using a model system of Characeae cells. The effect of ACh on conductance of K+ channels in Nitella flexilis cells and on the action potential generation in Nitellopsis obtusa cells after H+-ATPase inhibition, where repolarization occurs after the opening of outward rectifying K+ channels, was investigated. Voltage-clamp method based on only one electrode impalement was used to evaluate the activity of separate potassium ion transport system at rest. We found that ACh at high concentrations (1 mM and 5 mM) activates K+ channels as the main membrane transport system at the resting state involved in electrogenesis of Characeaen membrane potential. We observed that ACh caused an increase in duration of AP repolarization of cells in K+ state when plasmalemma electrical characteristics are determined by large conductance K+ channels irrespective of whether AP were spontaneous or electrically evoked. These results indicate interference of ACh with electrical cellular signalling pathway in plants.

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Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 82%

The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation

et al. 2012

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“…The reference electrode was immersed into the experimental solution near the cell and the microelectrode was inserted into the cell to measure electrical potential differences between outside and inside the intermodal cell. The details of experimental procedures, parameters and specifications of the experimental techniques were thoroughly described in previous studies [13][14][15].…”

Section: Object and Methodsmentioning

confidence: 99%

THE ACCUMULATION PROCESS of¹³⁷Cs AND⁹⁰Sr IN THE CELL OF NITELLOPSIS OBTUSA ALGAE

Marčiulionienė¹,

Jefanova²,

Sakalauskas³

et al. 2016

RadJ

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Abstract. In the present study, we investigated the accumulation of 137 Cs and 90 Sr in compartments of the Nitellopsis obtusa cells. The effect of Sr 2+ , Cs + and Ca 2+ , K + , which are chemical analogues of 90 Sr and 137 Cs, to the bioelectric parameters of these algae were studied simultaneously. The aim of this work was studying the penetration of 137 Cs and 90 Sr through regulating membrane barriers in the cells of starry stonewort (Nitellopsis obtusa). 137 Cs and 90 Sr are accumulated mainly in the cell membrane (75% to 92%) of these algae. The cell membrane as a cation exchanger regulates ion flow through the first cells diffusion barrier -its thick outer cytomembrane (the complex consisting of the cell wall and plasmalemma). Significantly, smaller amounts of 137 Cs and 90 Sr enter into the cytoplasm than in the cytomembrane, 10-20% and 3-10%, respectively. Analysis of the accumulation levels of 137 Cs and 90 Sr in the compartments of the Nitellopsis obtusa cells show their accumulation in the cell membrane as well as their active transport through outer and inner cytoplasmic membranes. Membrane potentials determined mainly by the gradient of the K + ions are doing an important regulatory function in this process. From the obtained data it results that cells of Nitellopsis obtusa algae can be a convenient radioecological model for the study of the accumulation of radionuclides in plants at the cellular level.

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“…All treated cells died after 2 h in 100 mM Cd 2+ solution (Kisnieriene et al 2009). Furthermore, electrical stimulation accelerated this process.…”

Section: Membrane Potential As An Indicator Of Early Effect Of Bioactmentioning

confidence: 97%

Electrical signalling in Nitellopsis obtusa: potential biomarkers of biologically active compounds

Kisnierienë

Lapeikaite

Pupkis

2018

Functional Plant Biol.

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The Nitellopsis obtusa (N.A.Desvaux) J.Groves cell provides a model system for complex investigation of instantaneous effects of various biologically active compounds (BC) on the generation of plant bioelectrical signals in vivo. Experimental evidence using multiple electrical signals as biomarkers of the effects of BC (acetylcholine, asparagine, glutamate, nicotine, aluminium, nickel and cadmium ions) is provided. The effect of BC on membrane transport systems involved in the cell excitability were tested by current clamp, voltage clamp and patch clamp methods. Membrane potential (MP) alterations and action potential (AP) patterns in response to BC were shown to represent the cell state. High discretisation frequency allows precise, high time resolution analysis of real-time processes measuring changes in excitation threshold, AP amplitude and velocity of repolarisation values after application of BC indicating the effect on ion channels involved in AP generation. Application of voltage clamp revealed that changes in AP peak value were caused not only by increment in averaged maximum amplitude of the Clcurrent, but in prolonged Clchannels' opening time also. The cytoplasmic droplet can serve as a model system in which the effects of BC on single tonoplast ion channel can be studied by patch clamping. Investigation of electrical cell-to-cell communication revealed evidence on the electrical signal transduction through plasmodesmata.

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The combined effect of Cd2+ and ACh on action potentials of Nitellopsis obtusa cells

Cited by 8 publications

References 33 publications

The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation

The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation

THE ACCUMULATION PROCESS of¹³⁷Cs AND⁹⁰Sr IN THE CELL OF NITELLOPSIS OBTUSA ALGAE

Electrical signalling in Nitellopsis obtusa: potential biomarkers of biologically active compounds

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The combined effect of Cd2+ and ACh on action potentials of Nitellopsis obtusa cells

Cited by 8 publications

References 33 publications

The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation

The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation

THE ACCUMULATION PROCESS of137Cs AND90Sr IN THE CELL OF NITELLOPSIS OBTUSA ALGAE

Electrical signalling in Nitellopsis obtusa: potential biomarkers of biologically active compounds

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THE ACCUMULATION PROCESS of¹³⁷Cs AND⁹⁰Sr IN THE CELL OF NITELLOPSIS OBTUSA ALGAE