The effect of alterations in fatty acid composition and cholesterol content on the nonelectrolyte permeability of Acholeplasma laidlawii B cells and derived liposomes

McElhaney, Ronald N.; Gier, J. De; Neut-Kok, E.C.M. Van Der

doi:10.1016/0005-2736(73)90376-3

Cited by 185 publications

(74 citation statements)

References 18 publications

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“…In light ofthe work of McElhaney et al (8), these results suggests that prior to acclimation S. commersonii plasma membranes were more fluid at low temperatures than those of S. tuberosum.…”

Section: Discussionmentioning

confidence: 73%

“…These Kp can be used as a probe of the membrane's physical status in vivo. Carruthers and Melchior (2) and McElhaney et al (8) reported that water and -nonelectrolyte permeation rates were positively correlated with liposome fluidity. A change in Kp for a given nonelectrolyte might then reflect a change in the physical status or composition of the plasma membrane lipids.…”

Section: Discussionmentioning

confidence: 99%

“…The Ea were similar to those of acclimated and nonacclimated S. commersonii. Although Kp were five to (8) found that nonelectrolyte permeation rates could be increased in Acholeplasma laidlawii B cells without affecting the Ea for permeation by incorporating more short chains or unsaturated fatty acids into the bilayer. An increase in unsaturation of total cellular membrane fatty acids upon exposure to low temperatures is common in plants (17).…”

Section: Discussionmentioning

confidence: 99%

“…This can affect such membrane functions as enzymic activity, and active and passive transport. Passive transport or permeability is directly affected by a change in the lipid composition or a change in the physical status of membranes in response to temperature changes (2,8). In this study, passive permeation of nonelectrolytes through the lipid portion of the plasma membrane was used as a nondestructive probe for examining the response of the membrane lipids to changes in temperature.…”

mentioning

confidence: 99%

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Temperature Response of Plasma Membranes in Tuber-Bearing Solanum Species

Fennell¹,

Li²

1986

Plant Physiol.

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ABSTRACIPermeability coefficients (Kp) of nonelectroytes were determined at several temperatures for nonacclimated and acclimated plasma membranes of the frost sensitive Solanum tuberosum and the frost resistant Solanum commersonii. In nonacclimated membranes, Kp were equal at 25°C for the two species. Kp decreased with decreased temperature in both species; however, the decrease was much greater in the frost sensitive S. tuberosum than in frost resistant S. commersonii.Kp increased with cold acclimation. After acclimation the temperature sensitivity of Kp or activation energy (Ea) for S. tuberosum was similar to that of S. commersonii; however, Kp for S. tuberosum were lower than those for S. commersonii at all temperatures.The differences in Kp and activation energy indicate plasma membrane differences between the species before acclimation. After acclimation there was still a difference between the plasma membranes of the two species as well as a difference between the nonacclimated and acclimated membranes of the same species.

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Section: Discussionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Temperature Response of Plasma Membranes in Tuber-Bearing Solanum Species

Fennell¹,

Li²

1986

Plant Physiol.

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“…The mechanisms producing heat induced drug tolerance (or sensitization) remain to be elucidated, but may involve membrane events. Cellular heat injury, it has been suggested, results from changes in the cell membrane (Bowler et al, 1973) and membrane fluidity is known to affect permeability and transport systems in intact mycoplasma cells (McElhaney et al, 1973). Alterations in membrane fluidity, causing disintegration of the integrity of the membrane, are thought to be involved in the hyperthermic cell death of E. coli (Yatvin, 1977).…”

Section: Thermal Tolerance and Drug Cytotoxicity At Elevated Temperatmentioning

confidence: 99%

The interaction of thermal tolerance with drug cytotoxicity in vitro

Morgan¹,

Honess²,

Bleehen³

1979

Br J Cancer

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The effect of preheating EMT6 cells in vitro on their response to cytotoxic agents of either 43 degrees C or 37 degrees C has been investigated. Preheating for 3 h at 40 degrees C produced measurable protection (thermal tolerance) to subsequent treatment for 1 h at 43 degrees C. This preheat treatment was further found to reduce cell killing by BLM and BCNU (drug tolerance) present during 1 h at 43 degrees C. In contrast, no such heat-induced drug tolerance was seen with ADR. An additional effect with ADR was the apparent elimination of heat-induced thermal tolerance at toxic drug doses. However, preheating under these conditions had no effect on the subsequent cytotoxicity of any of these drugs at 37 degrees C. Also, preheating for 1 h at 43 degrees C was found to sensitize cells to BLM and BCNU toxicity at 37 degrees C but to protect against ADR toxicity. The results are discussed in relation to known mechanisms of cell killing by heat and of thermal tolerance.

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