Temperature Response of Plasma Membranes in Tuber-Bearing <i>Solanum</i> Species

Fennell, Anne; Li, Paul H.

doi:10.1104/pp.80.2.470

Cited by 6 publications

(4 citation statements)

References 19 publications

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“…Kami-ike et al (16) examined the temperature dependence ofthe electrogenic pump of Chara corallina plasma membrane and found that the pump conductance was much more temperature dependent than the conductance ofthe passive channels. The Ea for permeation of urea and methylurea into living cells was surprisingly high, ranging from 10 to 18 kcal/mol in Solanum slices (13).…”

Section: Discussionmentioning

confidence: 99%

“…H+-effiux might also occur as a 'back-leak' via the pump. Not much data is available on the effects of temperature on active and passive processes in plant membranes (13,16). Kami-ike et al (16) examined the temperature dependence ofthe electrogenic pump of Chara corallina plasma membrane and found that the pump conductance was much more temperature dependent than the conductance ofthe passive channels.…”

Section: Discussionmentioning

confidence: 99%

“…Ion leakages caused by the effect of temperature on membrane lipids or proteins have been cited as potential causes of chilling injury or freezing damage (1 3, 28) nents of the PM or TN (13,21,28). One method to test hypotheses about the direct effect of temperature on the TN and PM is to examine the effects of temperature on isolated vesicles (11).…”

mentioning

confidence: 99%

“…Various membranes including the plasma membrane (PM2) and tonoplast (TN) have been hypothesized to be primary sites ofchilling injury because chilling temperatures may have direct effects on the fluidity, viscosity, or phase-state of membrane lipids (13,23,26 and references therein). Ion leakages caused by the effect of temperature on membrane lipids or proteins have been cited as potential causes of chilling injury or freezing damage (1 3, 28) nents of the PM or TN (13,21,28).…”

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confidence: 99%

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Effect of Temperature on the Plasma Membrane and Tonoplast ATPases of Barley Roots

DuPont

1989

Plant Physiol.

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The effect of temperature on the rate of proton transport and ATP hydrolysis by plasma membrane (PM) and tonoplast (TN) ATPases from barley (Hordeum vulgare L. cv CM 72) roots were compared. Rates of proton transport were estimated using the fluorescent amine dyes quinacrine and acridine orange. The ratio between rate of transport and ATP hydrolysis was found to depend on the dye, the temperature, and the type of membrane. The PM ATPase had an estimated Arrhenius energy of activation (Ea) of approximately 18 kilocalories per mole for ATP hydrolysis, and the Ea for proton transport was best estimated with acridine orange, which gave an Ea of 19 kilocalories per mole. The TN ATPase had an Ea for ATP hydrolysis of approximately 10 kilocalories per mole and the Ea for proton transport was best estimated with quinacrine, which gave an Ea of 10 kilocalories per mole. Acridine orange did not give an accurate estimate of Ea for the TN ATPase, nor did quinacrine for the PM ATPase. Reasons for the differences are discussed. Because it was suggested (AJ Pope, RA Leigh [1988] Plant Physiol 86: [1315][1316][1317][1318][1319][1320][1321][1322] that acridine orange interacts with anions to dissipate the pH gradient in TN vesicles, the complex effects of N03-on the TN ATPase were also examined using acridine orange and quinacrine and membranes from oats and barley. Fluorescent amine dyes can be used to evaluate the effects of ions, substrates, inhibitors, and temperature on transport but caution is required in using rates of quench to make quantitative estimates of proton fluxes.Various membranes including the plasma membrane (PM2) and tonoplast (TN) In order to measure the effect of variables such as temperature and ions on the TN and PM ATPase, it is important to find methods to measure transport that are not affected significantly by the conditions being tested. If temperatureand ion-insensitive methods are not available, then the effects oftemperature and ions on the techniques that are used must be defined. Fluorescent dyes such as acridine orange and quinacrine are commonly used in procedures for estimating rates of proton transport by membrane vesicles. However, Pope and Leigh (25) discussed various artifacts that occurred when acridine orange was used to measure pH gradients formed in TN vesicles obtained from red beet storage tissue, and Grzesiek and Dencher (15) described the artifacts that can occur when 9-aminoacridine is used to measure pH gradients. In this paper, the effects of temperature on ATP hydrolysis and on proton transport by the TN and PM ATPases of barley roots are described. During the course of the experiments it became clear that estimates of the effect of temperature on transport rates obtained with acridine orange or quinacrine could be quite different, and that the same dye might not be the best choice for the PM as for the TN. Some limitations on the use of quinacrine and acridine orange are described with respect to the two different membranes. MATERIALS AND METHODS Plant MaterialSeed...

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Section: Discussionmentioning

confidence: 99%