The effect of alcoholic mobile phase modifiers on retention and stereoselectivity on a commercially available cellulose-based HPLC chiral stationary phase: An unexpected reversal in enantiometric elution order

Enantiomeric separation of racemic sulphoxides on chiral stationary phases by gas and liquid chromatography

“…With the introduction of chemically modified cellulose, Ichida demonstrated a new type of stationary phase particularly suitable for the separation of chiral sulphoxides [15]. These stationary phases based on either cellulose or amylose are well known today as Chiralcel and Chiralpak, respectively, and have been used for a variety of sulphoxide separations [16,17]. From the above selection excellent results have been obtained with cellulose tribenzoate [18] and with amylose tris((S)-ct-methylbenzylcarbamate) [19].…”

Separation of Chiral Compounds on Polysaccharide Columns

“…In this particular case, a reversal of elution order for FMOC-d,l-leucine was observed when switching from the amylose-based Chiralpak IA column to cellulose-based Chiralpak IB and IC columns under otherwise identical chromatographic conditions. Occasionally, reversal of elution order with a given polysaccharide-based CSP can be enforced by appropriate changes in the mobile-phase conditions [79,87,88,[240][241][242][243]. Thus, changes in elution order were seen in alkane/alkane mobile phases upon changing from a shorter or less bulky alcohol to a longer or more bulky alcohol.…”

Chiral Discrimination Study for Polysaccharide‐Based Chiral Stationary Phases

“…These have stemmed mainly from studies in the dominant normal-phase mode. Commonly encountered and unexplained abnormal effects, such as different organic modifiers, temperatures, additives, and so on, caused a great deal of bewilderment; this significantly hindered systematic chiral method development [15][16][17][18][19][20][21][22][23][24][25].…”