The Effect of Administration of Extract from Areca Nut Seeds (Areca Catechu L) on the Estradiol and Estrus Cycle Balb/C Female Rats

Abstract

Population control is very important for individual and national welfare. Although various synthetic contraceptive agents are available, their use is associated with severe side effects. Therefore, an approach is taken to identify new antifertility agents from natural sources. This study was conducted to determine the effect of areca nut extract on estradiol levels and the estrus cycle of female balb / c mice. The experimental animal models used were fifteen adult female balb / c mice, aged 8-12 weeks, weight 20-40 g and induced for 1 week with water extract of areca nut with a dose of 1 g / 200 g body weight of mice (K1), 2 g / 200 g of body weight of mice (K2) and distilled water in the control group (K0). The estrus cycle was identified by a daily assessment of the relative ratio of nucleated epithelial cells, cornified squamous epithelial cells and leukocytes found at the time of vaginal swabs for 15 days continuously. At the proestrus stage blood is taken from the tail. Serum estradiol levels were determined by ELISA technique. There was a decrease in serum estradiol levels in the treatment group 1 (K1) 1.9 times compared to the control group (K0) and decreased estradiol levels in the treatment group 2 (K2) 5.29 times compared to the control group (K0). Changes in the duration of the estrus cycle occurred in treatment group 1 (K1) to be elongated and treatment group 2 (K2) became non-estrus, while the control group (K0) did not change the duration of the estrus cycle (normal). The decrease in serum estradiol levels affects the estrus cycle of mice (P = 0,000). The administration of areca nut extract has the effect of reducing serum estradiol levels and disrupting the duration of the estrous cycle of female balb / c mice. This finding has important implications for the development of contraception in women.

Diverse modulatory effects of sex steroids on depressive-like behavior in socially stressed female rats.

“…TAM ( n = 13) or saline VEH ( n = 14) injections were given to PRO and EST animals 1 hr before behavioral activities. Estradiol was administered to animals during the DIE phase due to evidence indicating a reduced level of estradiol in this phase (Aritonang et al, 2017). We hypothesized that estradiol administration would mitigate depressive-like behaviors induced by social isolation in the DIE phase, whereas TAM treatment would inhibit the beneficial effect of estrogen on depressive-like behavior.…”

“…DIE phase due to evidence indicating a reduced level of estradiol in this phase (Aritonang et al, 2017). We hypothesized that estradiol administration would mitigate depressive-like behaviors induced by social isolation in the DIE phase, whereas TAM treatment would inhibit the beneficial effect of estrogen on depressive-like behavior.…”

Studi Literatur Aktivitas Antiinflamasi sebagai Penyembuh Luka Tumbuhan Obat Terpilih Berdasarkan Ramuan Usada Tiwas Panggung

“…Tanin dalam Areca catechu L. merupakan proanthocyanidins yang juga disebut sebagai turunan flavanol, yang ada setelah bergabung dengan arecoline. Beberapa tanin telah diisolasi dan dikonfirmasi sebagai arecatannin A1, arecatannin A2, arecatannin A3, arecatannin B1, arecatannin B2, dan arecatannin C1 [26].…”

Increasing Natural Resistance Associated Macrophage Protein 1 serum level after Miana treatment in BALB/c induced Klebsiella pneumoniae experimental research

“…Survey found 74% of tuberculosis patients use traditional medicine as complementary. Although it has been used empirically for infectious treatment, there is not scientific demonstrations clear yet [ [6] , [7] , [8] , [9] , [10] ]. Therefore, usage of Miana becomes a potential to be studied and developed to understand the mechanisms of Miana leaves.…”