Increasing Natural Resistance Associated Macrophage Protein 1 serum level after Miana treatment in BALB/c induced Klebsiella pneumoniae experimental research

Abstract

Background Miana ( Coleus Scutellariodes [L] Benth) inhibits growth of bacterial pathogen inside macrophage. Objective The aim of this study is to determine the protein level of Natural Resistance Associated Macrophage Protein 1 (NRAMP1), after administration of Miana extracts in BALB/c mice induced Klebsiella pneumoniae . Methods This is an experimental study using animal model with post test-only controlled group design. Twenty healthy adult male BALB/c mice were randomly divided into four groups, negative control group (distilled water), Levofloxacin 100 mg/kg, injection intraperitoneal, first treatment group (Miana leaves extract/MLE 510 mg/kg) and second treatment group (Miana + levofloxacin). MLE were administered via gastric gavage for ten consecutive days. The blood was drawn from each mice on the first day, on the eight day of experiment (2 h after treatment), and at 10 days. The blood sample was examined by ELISA to determine the NRAMP1 protein level. Analysis of the number of lung tissue bacteria used Plate count agar to see the growth of Klebsiella pneumonia . Results NRAMP1 protein level in BALB/c mice after administration of Miana extract was increased significantly in after 10 days treatment (p < 0.0001). The highest increasing in protein levels was found in treatment group (Miana + levofloxacin) with an increase before treatment 3036,07 to 10010,30 pg/ml after treatment p < 0,0001. Conclusion NRAMP1 protein level in BALB/c mice were highest increasing in protein levels after administration of Miana extract and Levofloxacin compared Miana or Levofloxacin only and clinical impact proved a comparable effect on suppressing Klebsiella pneumoniae growth. The institutional protocol number of this study is 1010/UN4.6.4.5.31/PP36/2019.

The strong correlation between ADAM33 expression and airway inflammation in chronic obstructive pulmonary disease and candidate for biomarker and treatment of COPD

“…After incubation, the reaction was stopped by adding 50 µl of Stop Solution containing H 2 SO 4 to each well following which levels were measured within 30 min using the ELISA Reader 270 (Biomerieux, France) at a wavelength of 450 nm. The target soluble protein concentration was represented in ng/ml 7 , 12 – 14 .…”

Systemic lidocaine administration influences NF-kβ gene expression, NF-kβ and TNF- α protein levels on BALB/c mice with musculoskeletal injury

“…The number of mice was determined following the ethics of using experimental animals in health research, using the principles of replacement, reduction, and refinement [ 19 , 20 ]. Federer's formula was used to determine the number of mice required, i.e., (t-1) (n-1) ≥ 15, where t is the number of treatment groups and n is the number of mice required in this study, there were two treatment groups, and according to the above formula, at least eight mice were needed per group.…”

In vivo and in vitro inhibition effect of propolis on Klebsiella pneumoniae : A review

“…Historically, K. pneumoniae infection in immunocompromised individuals can cause serious illness. Hypervirulent variants increase the number of individuals susceptible to infection, including those who are healthy and immunocompetent [ 1 ].…”

“…Numerous studies have examined the use and effects of traditional medicines for various diseases [ [1] , [2] , [3] , [4] , [5] ], including the effects of herbal medicines on infectious diseases, such as candidiasis [ [6] , [7] , [8] ], typhoid fever [ [9] , [10] , [11] , [12] , [13] , [14] ], toxoplasmosis [ [15] , [16] , [17] ], gingivitis [ 18 , 19 ], vaginitis [ 20 ], and the human immunodeficiency virus (HIV) [ 21 , 22 ], and noninfectious diseases such as diabetes [ 23 ].…”