The Effect of Additional Autopolyploidization in a Slow Growing Cellulase Hyperproducer of Trichoderma

Toyama, Hideo; Toyama, Nobuo

doi:10.1007/978-1-4612-0217-2_66

Cited by 1 publication

(2 citation statements)

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“…The strain with higher Filter paper degrading ability was selected among those conidia using a double layer selection medium (Toyama et al, 2008). Toyama and Toyama (2001), who treated EMS treated isolate of T. reesei (M14-2) with 0.1% colchicine. They found that the cellulase production and growth rate of new isolate (M14-2B) were increased.…”

Section: Jmbt/vol2 Issuementioning

confidence: 99%

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Successive Construction of ?-Glucosidase Hyperproducers of Trichoderma Harzianum Using Microbial Biotechnology Techniques

El-Bondkly¹,

Abo-Shosha²

2010

JMBT

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In an attempt to construct superior Trichoderma harzianum isolates for improvement β-glucosidase productivity, induction of mutants was applied. After application of UV irradiation and Ethyl methane sulfonate (EMS), 461 isolates were obtained, out of them 99 after UV application and 362 isolates after EMS treatments. Five isolates (two after UV application and three after EMS treatments) were selected on the basis of their highly productivity of both enzymes to be treated with doses of colchicine (0.1% and 0.2%) as a second step of induction mutation. After colchicine treatments, 191 isolates were obtained, out of them 40 isolates after treating the wild type strain, 70 isolates after treating the two UV induced-mutants with colchicine and 81 isolates after treating the three EMS induced-mutants with colchicine .These isolates were tested for their CMCase and β-glucosidase productivities. One isolate (D1/4) proved to be the highest producer for the two enzymes, since it produced 160% and 186% CMCase and β-glucosidase, respectively, more than the original strain. In addition, highest DNA content and also highest amounts of CMCase and β-glucosidase were obtained after EMS-treatments followed by colchicine application.

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Section: Jmbt/vol2 Issuementioning

confidence: 99%

“…Colchicine binds to tubulin and then mitosis is arrested, inhibiting the normal distribution of chromosomes and yielding polyploid nuclei. Polyploids are also formed by colchicine treatment in T. reesei QM 9414 (Toyama and Toyama, 2001).…”

Section: Introductionmentioning

confidence: 99%

Successive Construction of ?-Glucosidase Hyperproducers of Trichoderma Harzianum Using Microbial Biotechnology Techniques

El-Bondkly¹,

Abo-Shosha²

2010

JMBT

View full text Add to dashboard Cite

show abstract

The Effect of Additional Autopolyploidization in a Slow Growing Cellulase Hyperproducer of Trichoderma

Cited by 1 publication

References 4 publications

Successive Construction of ?-Glucosidase Hyperproducers of Trichoderma Harzianum Using Microbial Biotechnology Techniques

Successive Construction of ?-Glucosidase Hyperproducers of Trichoderma Harzianum Using Microbial Biotechnology Techniques

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