The effect of acetylethyleneimine upon a strain of inactivated foot-and-mouth disease virus stored at 4� C

Staple, R. F.; Morrow, A. W.; Fletton, B W

doi:10.1007/bf01347973

Cited by 5 publications

(3 citation statements)

References 10 publications

(3 reference statements)

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“…Thus, observations on FMDV vaccines and specific cases of unstable virus strains such as Kenya 227/66 (6, 9) and ttong Kong (14) are consistent with the general pattern of the data presented here. It would seem worthwhile to investigate the thermal stabilities of other strains of FMD virus, particularly those which give relatively poor protection as vaccines.…”

Section: Thermal Stabilities Of 75 S Particlessupporting

confidence: 92%

“…This conclusion is substantiated by the work of ROWLANDS et al (9) and MOWAT et al (6) with the SAT2 serotype virus, Kenya 227/66, and STAPLE et al (14) with the "0" serotype virus, 0 Hong Kong. Their results indicate that inactivation with AEI reduced the stabilities of these viruses and, consequently, the potencies of the corresponding vaccines.…”

Section: Tr Doel and 1 ) J Baccar~isupporting

confidence: 62%

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Thermal stability of foot-and-mouth disease virus

Doel

Baccarini

1981

Archives of Virology

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The thermal stabilities of 146S component of seven strains of foot-and-mouth disease virus were found to differ considerably. Inactivation of infectivity with acetylethyleneimine (AEI) reduced the thermal stabilities of all but one of the viruses. Treatment of AEI inactivated and control virus preparations with glutaraldehyde stabilized 146S particles to a considerable extent, whereas treatment with dimethyl suberimidate was less effective. In similar experiments with 75S, natural empty particles, the thermal stabilities were lower than those of the corresponding 146 S particles. Treatment of 75S particles with AEI appeared to have no direct effect on the protein-protein interactions involved in 75S capsid integrity. As with 146S particles, glutaraldehyde stabilized 75S particles.

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Section: Thermal Stabilities Of 75 S Particlessupporting

confidence: 92%

Section: Tr Doel and 1 ) J Baccar~isupporting

confidence: 62%

Thermal stability of foot-and-mouth disease virus

Doel

Baccarini

1981

Archives of Virology

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“…Foot-and-mouth disease virus has been inactivated in cell culture fluids when exposed to a chemical agent, 2-bromo-ethylene-imine (BEI) (2,3,5,18). Influenza and mumps viruses have been inactivated by physical means through gamma-particle irradiation from a "°Co source t Journal paper no.…”

mentioning

confidence: 99%

Comparison of pseudorabies virus inactivated by bromo-ethylene-imine, 60Co irradiation, and acridine dye in immune assay systems

Sun

Gustafson

Scherba³

1978

J Clin Microbiol

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Pseudorabies virus infections among animals, especially swine, have become prevalent in the United States in the past few years. The disease in swine is now economically important. Test systems and antigens are being developed for use in control and disease suppression efforts. Pseudorabies virus was inactivated by three methods: chemically, with bromo-ethylene-imine; physically, with 60Co irradiation; and chemically and physically, with 3,9-diaminoacridine dye followed by exposure to white visible light. The antigenicities of the preparations were determined in the presence of specific antibody in immunodiffusion tests and through immunoelectrophoresis. The latter technique permitted quantitation of either antigen or antibody. In the electrophoretic patterns, the antigenic mass in bromo-ethylene-imine preparations was estimated to be 42 mg/ml, the same as in the untreated control material. After 60Co irradiation, 22 mg/ml was present, in comparison with 50 mg/ml in the untreated control antigen. In contrast, 67 mg/ml was present in the acridine dye-light-treated preparation, in comparison with 58 mg/ml in the untreated control material. A possible explanation for the acridine dye-light-treated preparation values is that photodynamic inactivation interferes with viral maturation during the replicative cycle within cells, with a resulting production of a greater amount of antigen, at least some of which is in the form of defective particles.

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