The effect of a tubercle bacillary “wax” on the response of guinea‐pigs to the injection of tubercle bacillary protein

The mechanism of the adjuvant effect of a tubercle bacillary lipid on the antibody response of rabbits to sheep erythrocytes

“…A " hard wax " fraction augmented antibody production in guinea-pigs when administered by intraperitoneal injection in saline with antigens under conditions in which granulomata were not prominent (Pound, 1955(Pound, , 1958. There appeared to be a systemic effect on the antibody-producing systems, perhaps related to the macrophage hyperplasia observed.…”

“…Tubercle bacillary lipid. The lipid was prepared from heat-killed Myobacteriurn tuberculosis as previously described (Pound, 1955). For use it was emulsified by grinding with pyrogen-free isotonic saline, subjected to ultrasonication and taken up to a concentration of 10 mg per ml.…”

Delayed Hypersensitivity

“…The differentiation can only be definitely achieved by the tedious method of transfer of delayed sensitivity to normal hosts using washed leucocytes from sensitized donors (Metaxas and Metaxas-Biihler (6)). Thus Pound (7) in attempting to repeat the work of Raffel (4) on the effect of tubercle bacillus wax on induction, in guinea pigs, of delayed sensitivity to tuberculoprotein, obtained reactions which he concluded to he predominantly of the Arthus type. Pound apparently did not attempt to transfer sensitivity to normal ani-n~Is by means of leucocyte suspensions from his sensitized guinea pigs.…”