The Effect of a Probiotic Containing Enterococcus faecium DSM 7134 on Redox and Biochemical Parameters in Chicken Blood

Abstract

it was postulated that administration of a probiotic to chickens can stimulate their antioxidant status while at the same time inhibiting oxidation processes. The objective of the study was to determine whether and how different levels and durations of application of a probiotic preparation containing live cultures of Enterococcus faecium and enriched with cholecalciferol and ascorbic acid influences indicators of lipid status and the redox reaction in the blood of broiler chickens. four hundred day-old ross 308 chickens were raised until their 42nd day of age. The animals were assigned to five experimental groups of 80 broilers each: a control group that did not receive the probiotic, T1 groups, which received the probiotic at 0.25 (e-0.25) or 0.1 g/l (e-0.1) throughout the rearing period, and T2 groups, which received the probiotic at the same levels, but during days 1-7, 15-21 and 29-35 of life. The probiotic preparation reduced the level of peroxides (looh), malondialdehyde (MDA), cholesterol (TC), low density (LDL) cholesterol, and non-esterified fatty acids (nefa), and the activity of catalase (caT), gamma-glutamyltransferase (ggT), acid phosphatase (ac), alkaline phosphatase (alp) and 3-hydroxybutyrate dehydrogenase (hbDh), while ferric reducing ability of plasma (frap) and glutathione (gSh + gSSg) levels were increased in the blood plasma of the chickens. The results obtained indicate that administration of this probiotic to chickens, especially in the amount of 0.25 g/l for the entire rearing period, stimulates synthesis of low-molecular-weight antioxidants responsible for maintaining redox homeostasis, without exerting a negative effect on liver metabolism in chickens.

Assessment of probiotic-supplementation on growth performance, lipid peroxidation, antioxidant capacity, and cecal microflora in broiler chickens

“…lactis, 10 9 cfu/ml) plus (L. plantarum, 10 12 cfu/ml) at 42 days old (Table 4). The current data coincide with the conclusion of Rajput et al (2013) and Ognik and Krauze (2016) who stated that probiotics increase the activities of antioxidant enzymes including superoxide dismutase, catalase, and glutathione peroxidase and reduce the concentration of MDA and uric acid. Zheng et al (2016) elucidate that the probiotic Enterococcus faecium bacteria promote resistance of biological macromolecules oxidation and take off hydroxyl radicals, herewith increasing the body's antioxidant capacity.…”

“…The increase in the relative weight of spleen is also in agreement with the findings of Willis et al (2007) who found that feeding broilers on probiotic caused increases in the relative weights of the spleen in the treated group. The increase in the relative weight of pancreas was also in agreement with the findings of Olnood et al (2015) who found that feeding broilers on probiotic caused increases in the relative weights of the pancreas of the treatment group. Subsequently, valuable effects of Lact.…”

“…In our experiments, the probiotics did not affect the relative weights of intestinal tracts of broilers after 42 days. Similar results were observed by Jin et al (1998), Huang et al (2004), andOlnood et al (2015) who demonstrated that the probiotic supplement Lactobacillus, Lactobacillus johnsonii, L. casei or L. acidophilus did not have an effect on organ weights and intestinal weight. Interestingly, the treated groups received the probiotic preparations either individually or combined and had relatively higher intestine weights compared to control group (Table 1), suggesting that mode of action of probiotic strains would be alike.…”

“…Free radicals could be produced in tissues and cells from outer sources (such as pollution, drugs, and food), internal (such as inflammation, diseases, or metabolism) or as a result of diminished protective capacity (Rice-Evans et al, 1991) and any excess in free radicals production can result in oxidative damage (Ghiselli et al, 2000;Rubio et al, 2016). Two known mechanisms have generated by organisms as an antioxidant defense system, one of them is based on the activity of antioxidant enzymes which neutralize free radicals and the other build on the subsistence of low-molecularweight antioxidants which directly interact with oxidant molecules leading to terminate the free radical chain reaction (Ognik et al, 2016(Ognik et al, , 2017.…”

Effects of dietary supplementation probiotic complex on growth performance, blood parameters, fecal harmful gas, and fecal microbiota in AA+ male broilers

“…Enterococcus faecalis was a lactic acid bacterium commonly found in the gastrointestinal tract of humans and animals ( Cao et al, 2013 ). The addition of E. faecalis to the diet promoted the growth of beneficial bacteria in the gut and inhibits harmful bacteria, thus maintaining a healthy gut and enabling the animal to achieved higher growth performance ( Adil and Magray, 2012 ; Ognik et al, 2017 ). Enterococcus faecalis had a strong colonization advantage over other probiotics and was therefore still widely used as a pharmaceutical and microbial additive ( Al-Balawi and Morsy, 2020 ; Hanifeh et al, 2021 ; Zhang et al, 2022 ).…”