Effects of dietary supplementation probiotic complex on growth performance, blood parameters, fecal harmful gas, and fecal microbiota in AA+ male broilers

Abstract

In this study, Bacillus subtilis, Clostridium butyricum and Enterococcus faecalis were made into a probiotic complex (PC). The PC was supplemented in AA+ male broilers’ diets to investigate the effects of PC on broiler growth performance, carcass traits, blood indicators, harmful gas emissions in feces and microbiota. Three hundred and sixty 1-day-old AA+ male broilers with an average initial body weight (data) were randomly divided into 3 dietary treatments of 6 replicates each, with 20 birds per replicate. The control group (T0) was fed a basal diet, while the test groups (T1 and T2) were supplemented with 0.025 and 0.05% PC in the basal diet, respectively. The trail was 42 days. The results showed that the supplementation of 0.05% PC significantly (p < 0.05) improved average daily gain (ADG) and average daily feed intake (ADFI) of broilers from 22 to 42 days and 1–42 days. Compared to the control group, the breast rate was significantly higher in T2, and the thymic index was significantly higher than that in T1 treatment (p < 0.05). The addition of PC had no significant effects on antibody potency in broiler serum (p > 0.05), but significantly increased albumin and total protein content in serum (p < 0.05). The addition of PC reduced H2S and NH3 emissions in the feces; the levels of Escherichia coli and Salmonella in the feces were significantly reduced and the levels of Lactobacillus were increased. And the most significant results were achieved when PC was added at 0.05%. Correlation analysis showed a significant positive correlation (p < 0.05) between the levels of E. coli and Salmonella and the emissions of H2S and NH3. Conclusion: Dietary supplementation with a 0.05% probiotic complex could improve the growth performance of broilers and also reduced fecal H2S and NH3 emissions, as well as fecal levels of E. coli and Salmonella, and increased levels of Lactobacillus. Thus, PC made by Bacillus subtilis, Clostridium butyricum and Enterococcus faecalis is expected to be an alternative to antibiotics. And based on the results of this trial, the recommended dose for use in on-farm production was 0.05%.

The effect of Limosilactobacillus fermentum 2i3 and 0.6% addition of humic substances on production parameters and the immune system of broilers

“…Our results showed that administration of L. fermentum 2i3 at a dose of 1 × 10 9 CFU/mL of drinking water had a positive effect on some production parameters such as feed conversion ratio and weekly weight gain. Likewise, several recent studies have reported that commercial probiotic products in which the concentration of probiotic bacteria used is at least 1 × 10 8 CFU/g of feed, demonstrably improve production parameters as well as the immune response of broiler chickens ( Rehman et al, 2020 ; Fesseha et al, 2021 ; Zou et al, 2022 ). Most authors believe that the beneficial effects of probiotics can be increased by selecting “strong strains”, combining 2 or more strains, gene manipulation, or even the increasingly popular combination of probiotics and other synergistic substances of natural origin.…”

Sodium dichloroisocyanurate: improving broiler health by reducing harmful microbial levels in the waterline

“…In this experiment, drinking water containing NaDCC significantly reduced NH 3 and H 2 S emissions from feces. Previous reports have shown that when the number of E. coli and Salmonella in feces is reduced, fecal NH 3 and H 2 S emissions are also reduced ( 43 ). Prolonged exposure of broilers to NH 3 has been shown to affect the structure of their cecal flora and, consequently, their growth performance ( 44 ).…”

Productive and Physiological Effects of Probiotics and Apple Vinegar on Broiler Chickens Under High Stocking Density

“…Probiotic are mono or mixed cultures of live microorganisms which can be administrated via different routes to broiler chickens, confer an improvement in intestinal microbiota, structure and mucosal immunity (Li et al, 2020;Wang et al, 2021). Moreover, a lot of previous studies illustrated the affirmative effects of probiotics in ameliorating digestibility and absorption of nutrients (Zhang et al, 2022), insulating harmful microorganisms (Gao et al, 2019;Biswas et al, 2022), enhancing growth performance (Arteaga et al, 2020;Zou et al, 2022), improving carcass characteristics and raising health parameters of broilers (Li et al, 2020;Khochamit et al, 2020). As mentioned, attention has been shifted to natural growth promoters as alternatives to antibiotics, which include organic acids (Elnesr et al, 2021).…”