The effect of a nanothickness coating on rough titanium substrate in the osteogenic properties of human bone cells

Abstract

This study evaluated the effect of a bioactive ceramic coating, in the nanothickness range, onto a moderately rough surface on the osteogenic behavior of human bone cells. The cells were harvested from the mandibular mental region and were cultured over Ti-6Al-4V disks of different surfaces: as-machined (M), alumina-blasted/acid etched (AB/AE), and alumina-blasted/acid-etched + 300-500 nm thickness amorphous Ca- and P-based coating obtained by ion beam-assisted deposition (Nano). The culture was then evaluated regarding cell viability, adhesion, morphology, immunolocalization of osteopontin (OPN) and alkaline phosphatase (ALP). The results showed that the surface treatment did not interfere with cell viability. At 1 day, AB/AE and Nano showed higher adhesion than the M surface (p < 0.001). Higher adhesion was observed for the M than the Nano surface at 7 days (p < 0.005). The percentage of cells showing intracellular labeling for OPN at day 1 was significantly higher for the Nano compared to M surface (p < 0.03). The percentage of ALP intracellular labeling at 7 days was significantly higher for the AB/AE compared to the M surface (p < 0.0065); no differences were detected at 14 days. Our results suggest that the presence of a thin bioactive ceramic coating on a rough substrate did not favor the events related to in vitro osteogenesis. (c) 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010.

Effect of titanium surface on secretion of IL1β and TGFβ1 by mononuclear cells

“…Though the effects of nano-and microrough titanium topography on osteoblast behavior have been extensively described, [18][19] assays in which monocyte/macrophage behavior is examined are useful determinants for elucidating several aspects of cell interactions with implant biomaterials. 6,11,20 Mononuclear cells, especially monocytes/macrophages, were chosen for this research due to their pivotal role in wound healing, 8 producing a range of molecules which have the potential to modulate the repair process, driving the duration and intensity of the inflammatory response.…”

Implant Coatings and Its Application in Clinical Reality

“…Although exciting theories for osteoconduction of CaP nanocoatings have been described and a vast number of promising in vitro and animal studies exist, the exact mechanism behind the biological response of CaP coatings is still unclear. It has also been reported that CaP nanocoating does not favor in vitro osteogenesis [ 36 ], and a recent well-controlled in vivo study in dogs could not either confi rm that CaP nanocoatings improve early bone integration [ 37 ].…”

Materials for Orthopedic Bioimplants: Modulating Degradation and Surface Modification Using Integrated Nanomaterials

“…Surfaces of the orthopedic bioimplants serve as the site of interaction for surrounding living tissue. Hence, it is imperative to enhance the biological performance of these bioimplants using bioactive nanomaterials [27][28][29][30][31][32]. Surface engineering using nanomaterials and other suitable coating technologies aims to design and develop the bioimplants with improved osseointegration for orthopedic applications [28,33,34].…”