The effect of a mono-component exogenous protease and graded concentrations of ascorbic acid on the performance, nutrient digestibility and intestinal architecture of broiler chickens

Cowieson, A. J.; Abdollahi, M.R.; Zaefarian, F.; Pappenberger, G.; Ravindran, V.

doi:10.1016/j.anifeedsci.2017.11.018

Cited by 20 publications

(20 citation statements)

References 22 publications

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“…The efficacy of exogenous microbial protease as a mono-component feed enzyme has been unequivocally demonstrated in recent years (Angel et al, 2011; Cowieson and Roos, 2014; Olukosi et al, 2015; Cowieson and Roos, 2016; Cowieson et al, 2018). These benefits have been reported on ileal amino acid digestibility, bird performance, and various indicators of gut health e.g., mucin, tight junctions, and nutrient transporter proteins (Cowieson et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

A mono-component microbial protease improves performance, net energy, and digestibility of amino acids and starch, and upregulates jejunal expression of genes responsible for peptide transport in broilers fed corn/wheat-based diets supplemented with xylanase and phytase

et al. 2019

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A total of 90 male Ross 308 broiler chicks were used in a digestibility and performance bioassay to explore the effect of reduction in dietary protein and digestible amino acids and inclusion of an exogenous mono-component protease on amino acid digestibility, net energy, jejunal gene expression, and bird performance. Four dietary treatments were created by the supplementation, or not, of 2 control diets with a mono-component exogenous protease. The control diets were corn/wheat/soybean meal-based and were formulated to be either nutritionally adequate or reduced in protein and amino acids (around 3%). The 2 control diets were supplemented with xylanase and phytase (2000 FYT). Treatments were therefore arranged as a 2 × 2 factorial design. The reduction in diet nutrient density had no significant effect on various experimental outcomes (including bird performance, amino acid digestibility, and net energy [NE]) that were measured with the exception of a reduction in the expression of aminopeptidase N and glucose transporter 2. However, the addition of exogenous protease resulted in an increase in weight gain and a reduction in feed conversion ratio (around 4%; P < 0.05) and an increase in the digestibility of several amino acids (P < 0.05) and starch (P = 0.06). Protease addition also resulted in an increase in both apparent metabolizable energy (AME) (+73 kcal/kg; P < 0.05) and NE (+107 kcal/kg; P < 0.05). The addition of exogenous protease to the diet also increased the jejunal expression of genes responsible for peptide transport (PepT2; P < 0.01) and starch digestion (sucrase isomaltase; P = 0.06). These results confirm the efficacy of exogenous protease in broiler diets that contain both xylanase and phytase and suggest substantial beneficial effects that extend beyond protein and amino acid nutrition. The effect of exogenous protease on energy partitioning, starch digestibility and the efficiency of nitrogen cycling is an area for further study.

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Section: Introductionmentioning

confidence: 99%

A mono-component microbial protease improves performance, net energy, and digestibility of amino acids and starch, and upregulates jejunal expression of genes responsible for peptide transport in broilers fed corn/wheat-based diets supplemented with xylanase and phytase

et al. 2019

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“…The secondary effects of exogenous proteases have been the focus of recent inquiries. Cowieson et al (2018) concluded that exogenous protease has beneficial effects in combination with ascorbic acid on broiler feed conversion, amino acid digestibility, and intestinal integrity. The mechanisms by which a protease contributes to positive gut health are not entirely clear and may be a combination of different factors (Cowieson and Roos, 2016).…”

Section: Introductionmentioning

confidence: 99%

Growth performance and intestinal health of broilers fed a standard or low-protein diet with the addition of a protease

Cardinal

Moraes²,

Andretta

et al. 2019

R. Bras. Zootec.

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Non-ruminants Full-length research article Growth performance and intestinal health of broilers fed a standard or low-protein diet with the addition of a protease ABSTRACT-We evaluated the effects of a protease supplementation on the growth performance and intestinal health of broilers. Cobb chicks (392; 1-42 d) were divided into four treatments (seven replicates of 14 birds each). There were two feed formulations: a standard diet (SD) and a low crude protein and digestible amino acids diet (Low CP&AA). The two diets were either supplemented (+P) or not (−P) with a protease (Jefo Protease; 1.25 g kg −1). Performance was evaluated by feeding phases (1-7, 8-21, 21-35, and 35-42 d). On day 28, ileum samples were analyzed by a morphometric index for histological alterations (I See Inside Scoring System-ISI). Broilers fed the Low CP&AA had a poor feed conversion ratio (FCR); however, the addition of the protease to the Low CP&AA positively affected FCR and body weight gain and promoted a performance similar to the group fed SD−P. Birds fed diets supplemented with the protease presented the best ISI morphological index, mainly as a result of the low number of alterations regarding the lamina propria, epithelial thickness, and enterocyte proliferation. It is possible to conclude that the enzyme improves feed conversion and lamina propria, epithelial thickness, and proliferation of enterocytes index of broiler chickens when added to a standard diet or with a low crude protein and digestible amino acids diet.

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“…Effects of protease supplementation are also divergent. In studies examining broiler chickens and turkeys, protease supplementation was reported to decrease (e.g., Walk et al., 2018; Borda-Molina et al., 2019), increase (e.g., Angel et al., 2011; Stefanello et al., 2016; Cowieson et al., 2018; Borda-Molina et al., 2019), or to have no effect (e.g., Boguhn et al., 2011; Kaczmarek et al., 2014; Erdaw et al., 2017; Borda-Molina et al., 2019) on pc AA digestibility.…”

Section: Introductionmentioning

confidence: 99%

Prececal amino acid digestibility and phytate degradation in broiler chickens when using different oilseed meals, phytase and protease supplements in the feed

et al. 2019

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The purpose of this study was to investigate the effects of phytase and protease supplementation on prececal (pc) amino acid (AA) digestibility, phytate (InsP6) degradation, and MEn concentration in diets using 3 oilseed meals as main protein sources in broiler chicken feed. The broiler chicken diets, which lacked mineral phosphorus, contained either soybean meal (SBM), SBM and rapeseed meal (SBM/RSM), or SBM and sunflower meal (SBM/SFM) as main protein sources. Diets were not supplemented with enzymes or supplemented with 1,500 or 3,000 FTU phytase/kg, or with 1,600 mg protease/kg. For diets containing SBM as the main protein source, the effects of phytase supplementation with and without monocalcium phosphate were also investigated. Data were obtained during 2 subsequent runs from days 14 to 22 and from days 23 to 31. Each diet was tested using 8 replicates with 4 replicates per run. For pc AA digestibility, no significant interactions were observed between main protein sources, enzyme supplementation, or addition of monocalcium phosphate except for Cys. Supplementation of 1,500 FTU phytase/kg increased pc digestibility of all AA. No differences in pc AA digestibility were observed between 1,500 and 3,000 FTU phytase/kg supplementation treatments. Prececal disappearance of InsP6 and pc P digestibility were greater in the high phytase supplementation treatment. Protease supplementation increased pc digestibility of all AA except for Cys when SBM/RSM was the main protein source. Supplementation of protease and 3,000 FTU phytase/kg increased MEn concentrations. The effect of phytase on pc AA digestibility was fully expressed at a lower supplementation level than needed for a maximized pc InsP6 disappearance and MEn concentration.

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The effect of a mono-component exogenous protease and graded concentrations of ascorbic acid on the performance, nutrient digestibility and intestinal architecture of broiler chickens

Cited by 20 publications

References 22 publications

A mono-component microbial protease improves performance, net energy, and digestibility of amino acids and starch, and upregulates jejunal expression of genes responsible for peptide transport in broilers fed corn/wheat-based diets supplemented with xylanase and phytase

A mono-component microbial protease improves performance, net energy, and digestibility of amino acids and starch, and upregulates jejunal expression of genes responsible for peptide transport in broilers fed corn/wheat-based diets supplemented with xylanase and phytase

Growth performance and intestinal health of broilers fed a standard or low-protein diet with the addition of a protease

Prececal amino acid digestibility and phytate degradation in broiler chickens when using different oilseed meals, phytase and protease supplements in the feed

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