Growth performance and intestinal health of broilers fed a standard or low-protein diet with the addition of a protease

Cardinal, Kátia Maria; Moraes, Mariana Lemos de; Andretta, Inés; Schirmann, Graciele Dalise; Belote, Bruna L.; Barrios, Miguel A.; Santín, Elizabeth; Ribeiro, Andréa Machado Leal

doi:10.1590/rbz4820180232

Cited by 16 publications

(14 citation statements)

References 41 publications

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“…There are mixed reports on body weight gain in broiler chickens fed low protein diets supplemented with protease. Similar to the findings of this study, Yu et al [10] and recently, Cardinal et al [16] reported no significant improvement in growth performance of birds fed low protein (5% and 6% CP/AA reduction, respectively) diets. However, Cardinal et al [16] observed a significant reduction in FCR from 1.73 to 1.60 in birds fed the protease supplemented low CP/AA diets.…”

Section: Source Of Variations/treatmentssupporting

confidence: 91%

“…Similar to the findings of this study, Yu et al [10] and recently, Cardinal et al [16] reported no significant improvement in growth performance of birds fed low protein (5% and 6% CP/AA reduction, respectively) diets. However, Cardinal et al [16] observed a significant reduction in FCR from 1.73 to 1.60 in birds fed the protease supplemented low CP/AA diets. In a similar study with a different protease (a mono-component protease), Dessimoni et al [17] reported similar findings i.e.…”

Section: Source Of Variations/treatmentssupporting

confidence: 91%

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Protease Complex Reduces Potentially Pathogenic Microbial Populations in the Ileum While Optimizing Performance of Broiler Chickens

Chowdhury¹,

Dhara²,

Dey³

et al. 2021

AVS

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A 42-day trial was conducted to assess the effects of a dietary protease on growth performance and ileum population of selected bacteria in broiler chickens fed standard diets and diets deficient in crude protein and digestible amino acids (CP/AA, ~5%) or apparent metabolizable energy (AME, 50 kcal/kg) or both. Reducing CP/AA and AME negatively affected average daily weight gain (ADG, P < 0.05) and feed conversion ratio (FCR, P < 0.05). Dietary protease improved BW at 42-d (P = 0.021, linear effect) of birds fed both standard and nutrient deficient diets but improved FCR (P = 0.0002) was only observed when supplemented to the standard diet. Serum protein concentration was not affected by the level of CP/AA and AME but decreased linearly with the level of protease (P = 0.02). Numbers of Escherichia coli¸ Salmonella spp. and Clostridium perfringens increased (P = 0.0001) with the decrease of dietary CP/AA and AME. In contrast, the numbers of studied bacteria linearly decreased (P = 0.0001) with dietary protease level. Dietary protease was found to decrease the numbers of Clostridium perfringens (P = 0.0001) when supplemented to either low CP/AA or low AME diets. It can be concluded that protease supplementation sustained the harvesting biomass in the experimental birds fed lower nutrient density diets and decreased the numbers of resident bacteria in the hind gut, some of which might be potential pathogens. Hence, the study documented not only the nutrient sparing effects but also the extra-proteinaceous effect in terms of gut health of dietary protease in broiler chickens.

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Section: Source Of Variations/treatmentssupporting

confidence: 91%

Section: Source Of Variations/treatmentssupporting

confidence: 91%

Protease Complex Reduces Potentially Pathogenic Microbial Populations in the Ileum While Optimizing Performance of Broiler Chickens

Chowdhury¹,

Dhara²,

Dey³

et al. 2021

AVS

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“…The ISI methodology is expected to improve on the deficiencies associated with current linear measures of gut villi and crypts dimensions, hence providing an adequate measure of gut mucosa status. Although lower ISI total scores have been described as indicators of better intestinal health ( Cardinal et al, 2019 ), it is possible that the higher ISI total scores observed in the BAS and OE treatments are indicative of constant basal intestinal inflammatory and proliferative responses. This is normal even in control birds, as in this study, raised under clean experimental conditions ( Sanches, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of organic acids–essential oils blend and oat fiber combination on broiler chicken growth performance, blood parameters, and intestinal health

2021

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This study evaluated the effect of organic acids-essential oils blend with or without oat hulls (OH) on growth performance, organ weights, blood parameters, gut morphology, microbiota, and short-chain fatty acids (SCFA) in broiler chickens. Day-old broiler chickens were randomly allocated to 4 dietary treatments consisting of 1) a corn-soybean meal-wheat based diet (BAS), 2) BAS + 0.05% bacitracin methylene disalicylate (BMD), 3) BAS + protected organic acids-essential oils at 300 g/1,000 kg of feed (OE), and 4) BAS + protected organic acids-essential oils at 300 g/1,000 kg of feed + 3% OH (OEOH), in 8 replicate groups. Feeding was in starter (d 0 to 14), grower (d 14 to 24), and finisher (d 24 to 36) phases. Body weight (BW), feed intake (FI), feed conversion ratio (FCR), and mortality were determined weekly. On d 36, 8 chickens per treatment were sampled for blood biochemistry, organ weights, cecal SCFA production, and microbiota. Treatments had no effect on FI and FCR at all phases. Both OE and OEOH treatments reduced ( P < 0.001) the body weight gain of birds at the starter phase. Birds fed the OEOH treatment had higher ( P < 0.001) gizzard weight, while those offered the BMD diet showed a tendency ( P = 0.08) to have higher cecal weight. Birds in the OEOH treatment recorded increased ileal villus height and villus height-to-crypt depth ratio, as well as reduced duodenal crypt depth, while birds in the OE treatment had increased jejunal villus height and villus height-to-crypt depth ratio. Both OEOH and OE treatments increased the number of goblet cells produced in the duodenum and jejunum. Treatments had no effect on SCFA concentrations. Birds in the OE treatment recorded the lowest concentration of blood urea ( P = 0.05) and cholesterol ( P < 0.05). Both OE and OEOH treatments increased ( P < 0.05) the relative abundance of potentially beneficial bacteria in the genus Firmicutes_unclassified, Ruminococcus , Turicibacter , and Erysipelotrichaceae_unclassified, while reducing ( P < 0.001) the relative abundance of potentially harmful Coprobacillus . Conclusively, both protected organic acids-essential oils blend and its combination with oat fibers show potential as tools to achieve antibiotics reduction in broiler production.

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“…Likewise, in our study, when the protein level decreased 10% in low protein groups, a decrease was found compared to the normal ones for BWG and carcass yield. Cardinal et al (4), in their studies, comparing growth performance and intestinal health in broiler groups with standard protein, the protein level reduced by 6% and protease added standard and low protein groups; reported that in the low protein group, BW, BWG and FCR were significantly adversely affected in 1-42 days compared to the standard protein group. These findings were consistent with the findings we obtained in our study that were determined in terms of protein.…”

Section: Discussionmentioning

confidence: 99%

Düşük proteinli broyler rasyonlarına çam kabuğu ekstraktı (Pinus pinaster) ilavesinin performans, bazı kan ve antioksidan parametreler ve bağırsak histomorfolojisi üzerine etkisi

Öztap¹,

Küçükersan

2023

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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This study was conducted to investigate the effects of Pinus pinaster extract (PPE) and encapsulated Pinus pinaster extract (EPPE) supplementation in normal and low protein broiler diets on performance, some blood and antioxidant parameters, and intestinal histomorphology. In the present study, PPE was covered with alginate in order to obtain EPPE. The present research was conducted during 41-days with 288 one-day-old male broiler chicks. Chicks were classified into two groups that had different protein levels, one of with normal, the other one with 10% low protein. Also, normal and low protein level groups were divided into one control and two trial groups. The amount of 100 mg/kg PPE was added to each trial group diet; and the same amount of EPPE was added to other trial group diets. Consequently, compared to PPE and EPPE groups with control, statistically significant differences were observed for body weight and body weight gain in terms of protein on 41-day results (P<0.05). For feed conversation ratio, better results were detected in PPE groups at 41 days (P<0.05). Besides that, statistically significant differences were found in breast meat thiobarbituric acid reactive substances (TBARS) values and intestinal histomorphology in PPE and EPPE groups compared to the control groups (P<0.05). In this study, the findings suggest that 100 mg/kg PPE and EPPE can be supplemented in normal and low protein broiler diets without any adverse effects and considered alternative aromatic substances for broiler rations.

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Growth performance and intestinal health of broilers fed a standard or low-protein diet with the addition of a protease

Cited by 16 publications

References 41 publications

Protease Complex Reduces Potentially Pathogenic Microbial Populations in the Ileum While Optimizing Performance of Broiler Chickens

Protease Complex Reduces Potentially Pathogenic Microbial Populations in the Ileum While Optimizing Performance of Broiler Chickens

Effect of organic acids–essential oils blend and oat fiber combination on broiler chicken growth performance, blood parameters, and intestinal health

Düşük proteinli broyler rasyonlarına çam kabuğu ekstraktı (Pinus pinaster) ilavesinin performans, bazı kan ve antioksidan parametreler ve bağırsak histomorfolojisi üzerine etkisi

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