The effect of a growth regulator Ribav-Extra on winter wheat seedlings exposed to heavy metals

Abstract

We studied the influence of a natural plant growth regulator Ribav-Extra and ions of heavy metals (HM) Pb 2+ , Cu 2+ , Zn 2+ and Ni 2+ on the physiological and biochemical indices of the winter wheat (Triticum aestivum L. ) cultivar 'Mironovskaya 808'. The seeds of wheat were treated with Ribav-Extra (10 ppm) and grown for 7 days on heavy metal salt solutions (10 μM or 1 mM). After that we recorded heavy metals accumulation, growth, generation of superoxide anion ( • O 2 − ), lipid peroxidation (LPO), and catalase (CAT) and ascorbate peroxidase (APOX) activity. It was found that 1 mM of HM in growth media increased heavy metals accumulation in wheat plants and inhibited the growth of roots and shoots. Both low and high concentrations of heavy metals stimulated • O 2 − production and oxidative damage in wheat seedlings. In most treatments, the toxic effect of heavy metals enhanced at higher concentration of metals. Pre-sowing treatment of seeds with Ribav-Extra decreased the negative impact of heavy metals by reducing oxidative stress which led to suppressed • O 2 − generation, lipid peroxidation intensity and catalase activity. This suggests that the wheat plants, whose seeds had been treated with Ribav-Extra, were more heavy metal-resistant than the untreated ones.Please use the following format when citing the article: Gruznova K. A., Bashmakov D. I., Miliauskienė J., Vaštakaitė V., Duchovskis P., Lukatkin A. S. 2018. The effect of a growth regulator Ribav-Extra on winter wheat seedlings exposed to heavy metals. Zemdirbyste-Agriculture, 105 (3): 227-234

Effects of inorganic salts concentration and alternative plant growth regulators on the in vitro organogenesis of a new hybrid Cymbidium

“…In this study, we document the first ever use of commercial Epin-Extra ® (with an 24-EpiB base) and RE as alternative PGRs. Previously, these natural PGRs were used only for seed treatment, spraying plants, or processing cuttings, and these treatments resulted in an enhanced tolerance to abiotic stresses (Lukatkin and Pogodina, 2012;Lukatkin et al, 2013a;2013b;Bashmakov et al, 2016;Gruznova et al, 2018). We also showed that these PGRs may be used as alternative PGRs in the in vitro culture of carnation (Dianthus caryophyllus L.) (Lukatkin et al, 2017).…”

“…A presowing treatment of wheat seeds with RE (10 ppm or 10 !3 %) decreased the negative impact of heavy metals (Cu, Zn, Ni, or Pb) by reducing oxidative stress by suppressing O 2•G generation, lipid peroxidation intensity, and catalase activity. This suggests that wheat plants whose seeds had been treated with RE were more heavy metal-resistant than untreated plants (Gruznova et al, 2018). The treatment of maize seeds with RE changed parameters related to oxidative stress (the generation of superoxide anion and the intensity of lipid peroxidation) and stimulated increased antioxidative activity in maize seedlings under unfavorable temperatures.…”

“…Usually, RE is used in the field or greenhouses for seed treatment, spraying plants, or processing cuttings. This preparation stimulates the synthesis of phytohormones, growth processes, and can increase resistance to stressors such as an increased tolerance of maize plants to high (45EC) and low (3EC) temperature stress (Lukatkin and Pogodina, 2012) and of wheat plants to heavy metals (Gruznova et al, 2018). When used at 10 5 %, during the in vitro clonal propagation of carnation (Dianthus caryo-phyllus L.), RE significantly stimulated shoot growth and significantly increased the number of internodes/shoot at 10 6 % (Lukatkin et al, 2017).…”

Cadmium Phytotoxicity, Tolerance, and Advanced Remediation Approaches in Agricultural Soils; A Comprehensive Review

“…An endogenous phenolic PGR such as salicylic acid (SA) governs an imperative role in plant physiological processes (photosynthesis, growth, and development), specifically under abiotic stresses, including Cd toxicity ( Tran and Popova, 2013 ; Gruznova et al, 2018 ). Ahmad et al (2011) highlighted that SA in initial growth stages assisted plants in mitigating increased damage caused by Cd toxicity by expressing specific proteins and defense-related enzymes ( Çanakci and Dursun, 2012 ; Roychoudhury et al, 2016 ).…”

Plant growth regulator effects on sunflower parents and F1 hybrids

“…The advantage of using plant growth regulators is their ability to increase the efficiency of plant nutrient utilization and maximize the fulfillment of genetic and physiological potentials (Jung et al, 2018), being non-toxic to plants and the environment and neutralizing the effects of heavy metals in soil (Gruznova, 2018). Growth regulators are responsible for important processes in the plant, boosting the resistance to abiotic stresses and stimulating the growth and development (Upreti et al, 2016).…”