The Ecoresponsive Genome of
            <i>Daphnia pulex</i>

Colbourne, John K.; Pfrender, Michael E.; Gilbert, Donald G.; Thomas, W. Kelley; Tucker, Abraham E.; Oakley, Todd H.; Tokishita, Shin-ichi; Aerts, Andrea; Arnold, Georg J.; Basu, Malay Kumar; Bauer, Darren J.; Cáceres, Carla E.; Carmel, Liran; Casola, Claudio; Choi, Jeong Hyeon; Detter, John C.; Dong, Qunfeng; Dusheyko, Serge; Eads, Brian D.; Fröhlich, Thomas; Geiler-Samerotte, Kerry; Gerlach, Daniel; Hatcher, Philip J.; Jogdeo, Sanjuro; Krijgsveld, Jeroen; Kriventseva, Evgenia V.; Kültz, Dietmar; Laforsch, Christian; Lindquist, Erika; Lopez, Jacqueline; Manak, J. Robert; Muller, Jean; Pangilinan, Jasmyn; Patwardhan, Rupali P; Pitluck, Samuel; Pritham, Ellen J.; Rechtsteiner, Andreas; Rho, Mina; Rogozin, Igor B.; Sakarya, Onur; Salamov, Asaf; Schaack, Sarah; Shapiro, Harris; Shiga, Yasuhiro; Skalitzky, Courtney; Smith, Zachary D.; Souvorov, Alexander; Sung, Way; Tang, Zuojian; Tsuchiya, Dai; Tu, Hank; Vos, Harmjan R.; Wang, Mei; Wolf, Yuri I.; Yamagata, Hideo; Yamada, Takuji; Ye, Yuzhen; Shaw, Joseph R.; Andrews, Justen; Crease, Teresa J.; Tang, Haixu; Lucas, Susan; Robertson, Hugh M.; Bork, Peer; Koonin, Eugene V.; Zdobnov, Evgeny M.; Grigoriev, Igor V.; Lynch, Michael; Boore, Jeffrey L.

doi:10.1126/science.1197761

Cited by 1,077 publications

(1,220 citation statements)

References 23 publications

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“…Daphnia magna (water flea; CladoceraCrustacea) (Figure 1) is a model organism widely advocated and employed in international standards and protocols for shortterm (acute) and long-term (chronic) exposure studies for determining substance toxicity, as well as a more general model for studies of genomic, ecological and evolutionary responses (Colbourne et al, 2011). Daphnia magna feeds on suspended particles of organic material such as unicellular algae, bacteria, yeast, and particulate biomass such as detritus, and plays critical roles as both consumer and prey in lentic systems (Lampert, 2006).…”

mentioning

confidence: 99%

Chronic Responses ofDaphnia magnaUnder Dietary Exposure to Leaves of a Transgenic (Event MON810) Bt–Maize Hybrid and its Conventional Near-Isoline

Holderbaum

Cuhra

Wickson

et al. 2015

Journal of Toxicology and Environmental Health, Part A

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Insect resistance is the second most common trait globally in cultivated genetically modified (GM) plants. Resistance is usually obtained by introducing into the plant's genome genes from the bacterium Bacillus thuringiensis (Bt) coding for insecticidal proteins (Cry proteins or toxins) that target insect pests. The aim of this study was to examine the hypothesis that a chronic, high-dose dietary exposure to leaves of a Bt-maize hybrid (GM event MON810, expressing a transgenic or recombinant Cry1Ab toxin), exerted no adverse effects on fitness parameters of the aquatic nontarget organism Daphnia magna (water flea) when compared to an identical control diet based on leaves of the non-GM near-isoline. Cry1Ab was immunologically detected and quantified in GM maize leaf material used for Daphnia feed. A 69-kD protein near Bt's active core-toxin size and a 34-kD protein were identified. The D. magna bioassay showed a resource allocation to production of resting eggs and early fecundity in D. magna fed GM maize, with adverse effects for body size and fecundity later in life. This is the first study to examine GM-plant leaf material in the D. magna model, and provides of negative fitness effects of a MON810 maize hybrid in a nontarget model organism under chronic, high dietary exposure. Based upon these results, it is postulated that the observed transgenic proteins exert a nontarget effect in D. magna and/or unintended changes were produced in the maize genome/metabolome by the transformation process, producing a nutritional difference between GM-maize and non-GM near-isoline.

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mentioning

confidence: 99%

Chronic Responses ofDaphnia magnaUnder Dietary Exposure to Leaves of a Transgenic (Event MON810) Bt–Maize Hybrid and its Conventional Near-Isoline

Holderbaum

Cuhra

Wickson

et al. 2015

Journal of Toxicology and Environmental Health, Part A

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“…The cytochromes P450 enzymes of aquatic crustaceans responsible for detoxification differed from those of fish (Koenig et al, 2012), indicating differences between invertebrates and vertebrates. The recent genome sequencing of vertebrates (Margulies and Birney, 2008) and invertebrates, including freshwater (Colbourne et al, 2011) and terrestrial (Adams et al, 2000;Whiteman et al, 2011) species has yielded new insights about the evolution of drug metabolizing enzymes (Asselman et al, 2012). For example, the cytochromes P450 (CYPs) appear to have undergone repeated duplications, resulting in multiple gene copies for similar enzymes (Hu et al, 2008).…”

Section: Pharmacological Mechanismsmentioning

confidence: 99%

A Pharm-Ecological Perspective of Terrestrial and Aquatic Plant-Herbivore Interactions

et al. 2013

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-We describe some recent themes in the nutritional and chemical ecology of herbivores and the importance of a broad pharmacological view of plant nutrients and chemical defenses that we integrate as "Pharm-ecology". The central role that dose, concentration, and response to plant components (nutrients and secondary metabolites) play in herbivore foraging behavior argues for broader application of approaches derived from pharmacology to both terrestrial and aquatic plant-herbivore systems. We describe how concepts of pharmacokinetics and pharmacodynamics are used to better understand the foraging phenotype of herbivores relative to nutrient and secondary metabolites in food. Implementing these concepts into the field remains a challenge but new modeling approaches that emphasize tradeoffs and the properties of individual animals show promise. Throughout we highlight similarities and differences between the historic and future applications of pharm-ecological concepts in understanding the ecology and evolution of terrestrial and aquatic interactions between herbivores and plants. We offer several pharm-ecology related questions and hypotheses that could strengthen our understanding of the nutritional and chemical factors that modulate foraging behavior of herbivores across terrestrial and aquatic systems.

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“…The functions and specific processes of the identified proteins were matched by searching Gene Ontology (www.geneontology.org). Metabolic pathways were identified by using KEGG PATHWAY (http://www.genome.jp/kegg/pathway.html) using the method described by Colbourne et al [24].…”

Section: Protein Identification and Bioinformaticsmentioning

confidence: 99%

Global effects of subchronic treatment of microcystin-LR on rat splenetic protein levels

Li¹,

Yan²,

Qiao³

et al. 2012

Journal of Proteomics

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The Ecoresponsive Genome of Daphnia pulex

Cited by 1,077 publications

References 23 publications

Chronic Responses ofDaphnia magnaUnder Dietary Exposure to Leaves of a Transgenic (Event MON810) Bt–Maize Hybrid and its Conventional Near-Isoline

Chronic Responses ofDaphnia magnaUnder Dietary Exposure to Leaves of a Transgenic (Event MON810) Bt–Maize Hybrid and its Conventional Near-Isoline

A Pharm-Ecological Perspective of Terrestrial and Aquatic Plant-Herbivore Interactions

Global effects of subchronic treatment of microcystin-LR on rat splenetic protein levels

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