The early stages of Saccharomyces cerevisiae yeast suspensions damage in moderate pulsed electric fields

Enhanced Extraction from Solid Foods and Biosuspensions by Pulsed Electrical Energy

“…The commonly reported values of field strength E, needed for disintegration of membranes in S. Cerevisiae cells, are rather high (E [ 7.5 kV/cm) [87,99], though smaller fields can also affect the structure of cells. For instance, the early stages of damage of the yeast cells were observed at E \ 7.5 kV/cm, at long time of PEF treatment [26,27]. The electroextraction of proteins from suspensions of S. cerevisiae was also observed at 3.2 kV/cm [34], but high efficiency (yield of 85%) required long extraction after PEF treatment ([4 h at 30°C).…”

Behavior of yeast cells in aqueous suspension affected by pulsed electric field

“…The nature of colloidal stability and main interaction forces between yeast cells are still under discussion [30][31][32]. S. cerevisiae cells show a strong tendency of aggregation even in the diluted suspensions (at volume concentration of ϕ ≈ 1%) [14,33]. That reflects the importance of the biopolymer bridging forces, related to the accumulation of specific glycoproteins on the surfaces of the cells [23].…”

“…The electric fields can provoke a considerable leakage of cytoplasmic ions in colloidal biosuspensions, which affects ionic concentration of the medium and its electrical conductivity [12][13][14]. It was shown that application of the pulsed electric fields (PEF) with a typical field strength E of 5-50 kV/cm and microsecond duration cause creation and growth of pores (electroporation) and the damage of the cell membranes.…”

S. cerevisiae fermentation activity after moderate pulsed electric field pre-treatments

“…1) during the PEF treatment and continued to increase after the PEF treatment (t N 16.5 min). It evidently reflected the leakage of intracellular ionic components, caused by electroporation of S. cerevisiae cells during and after the PEF treatment [15,[20][21][22][23]. Noticeable changes of σ were observed even after PEF treatment of the sample with lower electric field strength (E = 100 V/cm).…”