Behavior of yeast cells in aqueous suspension affected by pulsed electric field

Zakhem, Henri El; Lanoisellé, Jean‐Louis; Lebovka, Nikolaï; Nonus, M.; Vorobiev, Eugène

doi:10.1016/j.jcis.2006.04.055

Cited by 46 publications

(28 citation statements)

References 59 publications

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“…This could be used to follow the behavior along a train of pulses [8,12,13] In the flow bipolar electropulsator (Deex Bio, Betatech), a different approach was used. A Clamp-on current logger (Chauvin Arnoux) was used to monitor by a contact less approach the current that was delivered to the pulsing chamber.…”

Section: A Conductance Changes Of the Cell Suspensionmentioning

confidence: 99%

Conductance Dynamics to Monitor and Control Cell Electropermeabilization

Teissié

2016

IFMBE Proceedings

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Abstract-Membrane electropermeabilization is observed when electric pulses over a critical strength are delivered on a cell suspension. This is associated with a time dependent change in the suspension conductance. The magnitude and the kinetics of the conductivity changes can be used to get access to the molecular event supporting the membrane electropermeabilization. In Bioprocesses, where extraction results from electropermeabilization, the control of the conductivity change of the cell suspension allows the definition of optimized safe conditions.

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Section: A Conductance Changes Of the Cell Suspensionmentioning

confidence: 99%

Conductance Dynamics to Monitor and Control Cell Electropermeabilization

Teissié

2016

IFMBE Proceedings

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“…17 Negative effects of pulsed high electric field (2-5 kV/cm) on yeast cells in aqueous suspension were also investigated. 19 On the other hand, some cells like Bacillus stearothermophilus is known to show strong resistance to high electric field up to 40 kV/cm. There is also a report that the application of electric field to living cells can promote metabolic responses.…”

Section: Introductionmentioning

confidence: 99%

Influences of electric field on living cells in a charged water-in-oil droplet under electrophoretic actuation

Noh

et al. 2011

Biomicrofluidics

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We experimentally investigate the effects of high electric field on living cells inside a charged droplet under electrophoretic actuation. When an aqueous droplet suspended in a dielectric liquid contacts with electrified electrode, the droplet acquires charge. This charged droplet undergoes electrophoretic motion under strong electric field (1–3 kV/cm), which can be used as a droplet manipulation method in biomicrofluidic applications. However, because strong electric field and use of dielectric oil can be a harmful environment for living cells, the biological feasibilities have been tested. Trypan blue test and cell growth test have been performed to check the viability and proliferation of cells in a droplet under various electric field strengths and actuation times. We have not observed any noticeable influence of electric field and silicone oil on the viability and proliferation of cells, which indicates that electrophoresis could be safely used as a manipulation method for a droplet containing living biological system.

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“…The temperature increases rapidly with the pulse duration and/or electric field strength resulting in a process where thermal effects on micro-organisms, enzymes and quality determining compounds present in the product interfere with the electroporation effects. To control the product temperature, intermediate cooling sections are implemented in some continuous-flow PEF equipment or a pausing time between pulses is incorporated in batch processing (El Zakhem et al, 2006a), but this is not incorporated in the contours as shown in Fig. 1.3.…”

Section: Effect Of the Choice Of Pulse Parameters With Respect To Heatmentioning

confidence: 99%

“…However, application of the pulses took up to hours, as a temperature increase of the sample was avoided in these studies (El Zakhem et al, 2006a;El Zakhem et al, 2007). These moderate intensity conditions have been extensively studied as a pre-step in mass transfer processes in food-and biotechnology, aiming to mildly disintegrate plant cells.…”

Section: Knowledge Gapsmentioning

confidence: 99%

“…The use of moderate intensity PEF conditions has been studied in biology applications for gene transfer, and showed electroporation effects in microorganisms, when exposure time was long enough (Eynard et al, 1998). However, in most studies the application of the pulses took up to hours, as a temperature increase of the sample was avoided (El Zakhem et al, 2006a;El Zakhem et al, 2007). Although high intensity PEF treatment aims for minimal thermal effects, the temperature of the treated medium increases as the result of Ohmic, also called Joule, heating.…”

Section: Introductionmentioning

confidence: 99%

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Moderate and high intensity pulsed electric fields : Effect on microbial inactivation, shelf life and quality of fruit juices

Timmermans¹

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Behavior of yeast cells in aqueous suspension affected by pulsed electric field

Cited by 46 publications

References 59 publications

Conductance Dynamics to Monitor and Control Cell Electropermeabilization

Conductance Dynamics to Monitor and Control Cell Electropermeabilization

Influences of electric field on living cells in a charged water-in-oil droplet under electrophoretic actuation

Moderate and high intensity pulsed electric fields : Effect on microbial inactivation, shelf life and quality of fruit juices

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