The EAR-motif of the Cys2/His2-type Zinc Finger Protein Zat7 Plays a Key Role in the Defense Response of Arabidopsis to Salinity Stress

Ciftci-Yilmaz, Sultan; Morsy, Mustafa; Song, Liang; Coutu, Alicia; Krizek, Beth A.; Lewis, Michael; Warren, Daniel; Cushman, John C.; Connolly, Erin L.; Mittler, Ron

doi:10.1074/jbc.m611093200

Cited by 263 publications

(225 citation statements)

References 36 publications

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“…Yeast two-hybrid analysis was performed at the University of Wisconsin Molecular Interaction Facility, as described in Ref. 35. Testing for protein-protein interaction by growth on selection media and ␤-galactosidase activity was performed as described in Ref.…”

Section: Methodsmentioning

confidence: 99%

“…Testing for protein-protein interaction by growth on selection media and ␤-galactosidase activity was performed as described in Ref. 35. Analysis of microarray data, available on the World Wide Web, was performed as previously described (36).…”

Section: Methodsmentioning

confidence: 99%

“…As prey, we used a library constructed from Arabidopsis seedlings subjected to different abiotic stresses (35). Approximately 18 million clones were screened via mating.…”

Section: Thermotolerance Of Knock-out Plants Deficient In Mbf1cmentioning

confidence: 99%

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The Transcriptional Co-activator MBF1c Is a Key Regulator of Thermotolerance in Arabidopsis thaliana

Suzuki

Bajad

Shuman

et al. 2008

Journal of Biological Chemistry

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256

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The ability of an organism to acclimate to its environment is a key determinant in its global distribution and capacity to compete with other organisms. The heat stress response, a highly conserved environmental and developmental program in eukaryotic and prokaryotic organisms, is an important component of the acclimation response of plants. Previous studies have shown that heat shock transcription factors play an important role in thermotolerance in plants and other organisms, controlling the expression of different heat shock proteins and detoxifying enzymes. In contrast, although several other pathways, involving ethylene, salicylic acid (SA), and trehalose, were recently shown to play a central role in thermotolerance in plants, a key regulator of these responses was not identified. Here we report that the highly conserved transcriptional coactivator, MBF1c (multiprotein bridging factor 1c), is a key regulator of thermotolerance in Arabidopsis thaliana. MBF1c protein accumulates rapidly and is localized to nuclei during heat stress. MBF1c is required for thermotolerance and functions upstream to SA, trehalose, ethylene, and pathogenesis-related protein 1 during heat stress. In contrast, MBF1c is not required for the expression of transcripts encoding HSFA2 and different heat shock proteins. Interestingly, MBF1c interacts with TPS5 (trehalose phosphate synthase 5), which is also heat-inducible, and mutants deficient in TPS5 are thermosensitive. Our results provide evidence for the existence of a tightly coordinated heat stress-response network, involving trehalose-, SA-, and ethylene-signaling pathways, that is under the control of MBF1c.Thermotolerance is an essential component of the acclimation response of different organisms (1-6). It is generally divided into acquired thermotolerance (i.e. the ability to acquire tolerance to otherwise lethal heat stress) and basal thermotolerance (i.e. the inherent ability to survive temperature above the optimal growth) (5). Previous studies have shown that heat shock transcription factors (HSFs) 2 play an important role in thermotolerance in plants and other organisms, regulating heat shock proteins (HSPs) as well as different acclimation and detoxification proteins (7-10). In contrast, although several other pathways, involving ethylene, salicylic acid (SA), and trehalose, have recently been proposed to play an important role in thermotolerance in plants (5, 11-15), a key regulator of these responses was not identified.MBF1 (multiprotein bridging factor 1) is a highly conserved transcriptional co-activator involved in the regulation of diverse processes, such as endothelial cell differentiation, hormone-regulated lipid metabolism, central nervous system development, and histidine metabolism (16 -19). MBF1 proteins from different organisms interact with transcription factors, such as c-Jun, GCN4, and ATF1, or with different hormone receptors and link them with the TATA-binding protein (16 -20). The flowering plant Arabidopsis thaliana contains three different genes e...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The Transcriptional Co-activator MBF1c Is a Key Regulator of Thermotolerance in Arabidopsis thaliana

Suzuki

Bajad

Shuman

et al. 2008

Journal of Biological Chemistry

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256

241

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“…Y2H assays were performed as described (Ciftci-Yilmaz et al, 2007). Fulllength cDNA clones were first cloned into pDONR207 and, subsequently, the full-length gene was introduced into the Y2H vector pXDGATCY86 as bait or pGADT-Rec7 as prey (Ciftci-Yilmaz et al, 2007).…”

Section: Y2h Subcellular Localization and Bifc Assaysmentioning

confidence: 99%

“…Fulllength cDNA clones were first cloned into pDONR207 and, subsequently, the full-length gene was introduced into the Y2H vector pXDGATCY86 as bait or pGADT-Rec7 as prey (Ciftci-Yilmaz et al, 2007). Positive interactions were quantified using the b-galactosidase assay kit (Pierce Biotechnology).…”

Section: Y2h Subcellular Localization and Bifc Assaysmentioning

confidence: 99%

Agrobacterium May Delay Plant Nonhomologous End-Joining DNA Repair via XRCC4 to Favor T-DNA Integration

et al. 2012

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Agrobacterium tumefaciens is a soilborne pathogen that causes crown gall disease in many dicotyledonous plants by transfer of a portion of its tumor-inducing plasmid (T-DNA) into the plant genome. Several plant factors that play a role in Agrobacterium attachment to plant cells and transport of T-DNA to the nucleus have been identified, but the T-DNA integration step during transformation is poorly understood and has been proposed to occur via nonhomologous end-joining (NHEJ)-mediated doublestrand DNA break (DSB) repair. Here, we report a negative role of X-RAY CROSS COMPLEMENTATION GROUP4 (XRCC4), one of the key proteins required for NHEJ, in Agrobacterium T-DNA integration. Downregulation of XRCC4 in Arabidopsis and Nicotiana benthamiana increased stable transformation due to increased T-DNA integration. Overexpression of XRCC4 in Arabidopsis decreased stable transformation due to decreased T-DNA integration. Interestingly, XRCC4 directly interacted with Agrobacterium protein VirE2 in a yeast two-hybrid system and in planta. VirE2-expressing Arabidopsis plants were more susceptible to the DNA damaging chemical bleomycin and showed increased stable transformation. We hypothesize that VirE2 titrates or excludes active XRCC4 protein available for DSB repair, thus delaying the closure of DSBs in the chromosome, providing greater opportunity for T-DNA to integrate.

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Toward integration of a systems‐based approach for understanding drought stress in plants

Hussain

Ahsan

Sornaraj

et al. 2016

Water Stress and Crop Plants

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The EAR-motif of the Cys2/His2-type Zinc Finger Protein Zat7 Plays a Key Role in the Defense Response of Arabidopsis to Salinity Stress

Cited by 263 publications

References 36 publications

The Transcriptional Co-activator MBF1c Is a Key Regulator of Thermotolerance in Arabidopsis thaliana

The Transcriptional Co-activator MBF1c Is a Key Regulator of Thermotolerance in Arabidopsis thaliana

Agrobacterium May Delay Plant Nonhomologous End-Joining DNA Repair via XRCC4 to Favor T-DNA Integration

Toward integration of a systems‐based approach for understanding drought stress in plants

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