The duck enteritis virus early protein, UL13, found in both nucleus and cytoplasm, influences viral replication in cell culture

Hu, Xiao; Wang, M.; Chen, S.; Jia, Renyong; Zhu, Dekang; Liu, M.; Yang, Qiao; Sun, Kunfeng; Chen, X.; Cheng, Anchun

doi:10.3382/ps/pex043

Cited by 20 publications

(14 citation statements)

References 45 publications

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“…IE genes are expressed in the presence of CHX although the transcription of E and L genes is suppressed [31]. We also treated DEV-infected cells with GCV and examined UL54 (an IE gene) [32], US2 (an L gene) [33], UL13 (an E gene) [34], and β-actin as controls. We found that UL21 gene expression was inhibited in the groups treated with GCV and CHX.…”

Section: Discussionmentioning

confidence: 99%

Duck enteritis virus UL21 is a late gene encoding a protein that interacts with pUL16

et al. 2020

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Background: pUL21 is a conserved protein of Alphaherpesvirinae that performs multiple important functions. The Cterminus of pUL21 in other members of this subfamily has RNA-binding ability; this domain contributes to pseudorabies virus (PRV) retrograde axonal transport in vitro and in vivo and participates in newly replicated viral DNA packaging and intracellular virus transport. However, knowledge regarding duck enteritis virus (DEV) pUL21 is limited. Results: We verified that DEV UL21 is a γ2 gene that encodes a structural protein. Moreover, we observed that pUL21 localized to the nucleus and cytoplasm. DEV pUL21 interacted with pUL16 and formed a complex in transfected human embryonic kidney (HEK) 293 T cells and DEV-infected duck embryo fibroblasts (DEFs). These results were further confirmed by CO-IP assays. Conclusions: The DEV UL21 gene is a late gene, and pUL21 localizes to the nucleus and cytoplasm. DEV UL21 is a virion component. In addition, pUL21 can interact with pUL16. These findings provide insight into the characteristics of UL21 and the interaction between pUL21 and its binding partner pUL16. Our study enhances the understanding of DEV pUL21.

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Section: Discussionmentioning

confidence: 99%

Duck enteritis virus UL21 is a late gene encoding a protein that interacts with pUL16

et al. 2020

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“…IE genes are expressed in the presence of CHX, though transcription of E and L genes is suppressed (36). We also treated DEV-infected cells with GCV, a DNA polymerase inhibitor, and we examined UL54 (an IE gene) (37), US2 (an L gene) (38), UL13 (an E gene) (39), and β-actin as controls. We found that UL21 gene expression was inhibited in the groups treated with GCV and CHX.…”

Section: Discussionmentioning

confidence: 99%

Duck enteritis virus UL21 is a late gene and encodes a protein that interacts with pUL16

Yang

Wang

Zeng

et al. 2019

Preprint

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Background pUL21 is a conserved protein of Alphaherpesvirinae and exhibits multiple important functions. The C-terminus of pUL21 in other members of this subfamily has RNA-binding ability; this domain contributes to pseudorabies virus (PRV) retrograde axonal transport in vitro and in vivo and participates in newly replicated viral DNA packaging and intracellular virus transport. However, little is known about duck enteritis virus (DEV) pUL21. Methods In our study, recombinant pUL21 was expressed using apET-32c (+) vector in Escherichia coli BL21 cells induced with 0.4 mM isopropyl β-D-thiogalactoside for 8 h at 30°C. The antibody for indirect immunofluorescence (IFA) and western blotting (WB) analysis were then prepared. Pharmacological inhibition, WB and quantitative reverse transcription PCR (RT-qPCR) were performed. A coimmunoprecipitation (CO-IP) assay was conducted to test the interaction between pUL21 and pUL16. Results We verified that DEV UL21 is a γ2 gene and encodes a structural protein. Moreover, we observed that pUL21 localized to the nucleus and cytoplasm. DEV pUL21 interacted with pUL16 and formed a complex in transfected human embryonic kidney (HEK) 293T cells and DEV-infected duck embryo fibroblasts (DEFs). These results were further confirmed by CO-IP assays. Conclusions The DEV UL21 gene is a late gene, and the pUL21 localizes to the nucleus and cytoplasm. DEV UL21 is a virion component. In addition, pUL21 can interacts with pUL16. These ﬁndings provide insight into the characteristics of UL21 and the interaction between pUL21 and binding partner pUL16. Our study enhances understanding of DEV pUL21.

show abstract

“…IE genes are expressed in the presence of CHX although the transcription of E and L genes is suppressed (36). We also treated DEV-infected cells with GCV and examined UL54 (an IE gene) (37), US2 (an L gene) (38), UL13 (an E gene) (39), and βactin as controls. We found that UL21 gene expression was inhibited in the groups treated with GCV and CHX.…”

Section: Discussionmentioning

confidence: 99%

Duck enteritis virus UL21 is a late gene and encodes a protein that interacts with pUL16

Yang

Wang

Zeng

et al. 2019

Preprint

View full text Add to dashboard Cite

Background pUL21 is a conserved protein of Alphaherpesvirinae that performs multiple important functions. The C-terminus of pUL21 in other members of this subfamily has RNA-binding ability; this domain contributes to pseudorabies virus (PRV) retrograde axonal transport in vitro and in vivo and participates in newly replicated viral DNA packaging and intracellular virus transport. However, knowledge regarding duck enteritis virus (DEV) pUL21 is limited. Methods In our study, recombinant pUL21 was expressed using an pET-32c (+) vector in Escherichia coli BL21 cells induced with 0.4 mM isopropyl β-D-thiogalactoside for 8 h at 30°C. The antibody used for the indirect immunofluorescence (IFA) and western blotting (WB) analysis were prepared. Pharmacological inhibition, WB and quantitative reverse transcription PCR (RT-qPCR) were performed. A coimmunoprecipitation (CO-IP) assay was conducted to test the interaction between pUL21 and pUL16. Results We verified that DEV UL21 is a γ2 gene that encodes a structural protein. Moreover, we observed that pUL21 localized to the nucleus and cytoplasm. DEV pUL21 interacted with pUL16 and formed a complex in transfected human embryonic kidney (HEK) 293T cells and DEV-infected duck embryo fibroblasts (DEFs). These results were further confirmed by CO-IP assays. Conclusions The DEV UL21 gene is a late gene, and pUL21 localizes to the nucleus and cytoplasm. DEV UL21 is a virion component. In addition, pUL21 can interact with pUL16. These ﬁndings provide insight into the characteristics of UL21 and the interaction between pUL21 and its binding partner pUL16. Our study enhances the understanding of DEV pUL21. Keywords: Duck enteritis virus, UL21, UL16, late gene, interaction

show abstract

The duck enteritis virus early protein, UL13, found in both nucleus and cytoplasm, influences viral replication in cell culture

Cited by 20 publications

References 45 publications

Duck enteritis virus UL21 is a late gene encoding a protein that interacts with pUL16

Duck enteritis virus UL21 is a late gene encoding a protein that interacts with pUL16

Duck enteritis virus UL21 is a late gene and encodes a protein that interacts with pUL16

Duck enteritis virus UL21 is a late gene and encodes a protein that interacts with pUL16

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