The dual role of mevalonate in the cell cycle.

Quesney-Huneeus, V; Galick, Heather; Siperstein, Marvin D.; Erickson, SK; Spencer, Thomas A.; Ja, Nelson

doi:10.1016/s0021-9258(18)33268-x

Cited by 179 publications

(7 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Co-culture with endothelium, however, increased this fraction to 63%, whereas co-culture with other ceils on microcarriers (control for nonspecific effects) did not stimulate DNA synthesis. Although DNA synthesis was stimulated, the SMCs did not proliferate significantly (i.e., complete the cell cycle) in lipoprotein-deficient medium, presumably because of a limited supply of exogenous sterols, which prevented completion of mitosis (35), but if sufficient lipoprotein was present (2 or 5% PDS), SMC proliferation occurred in the presence of endothelial cells (Fig. 5, a and b).…”

Section: Mechanisms Of Endothelial-specific Stimulation Of Ldl Metabolism In Smcsmentioning

confidence: 99%

Metabolic cooperation between vascular endothelial cells and smooth muscle cells in co-culture: changes in low density lipoprotein metabolism.

Davies¹,

Truskey²,

Warren³

et al. 1985

The Journal of Cell Biology

View full text Add to dashboard Cite

A microcarrier co-culture system for aortic endothelial cells and smooth muscle cells (SMCs) was developed as a model for metabolic interactions between cells of the vessel wall. Low density lipoprotein (LDL) metabolism in SMCs was significantly influenced by coculture with endothelium. The numbers of high affinity receptors for LDL was increased more than twofold (range, 2.1-5.6), with concomitant increases in LDL receptor-mediated endocytosis and degradation. These effects reached a plateau at an endothelial celI/SMC ratio of 1. Kinetic analysis of the endocytic pathway for LDL in SMCs indicated that, in co-culture with endothelium, there was no alteration in the binding affinity of LDL to its receptors but that the internalization rate constant declined and the rate constant for degradation increased. This analysis suggested that the formation and migration of endocytic vesicles was the rate-limiting step of enhanced LDL metabolism under co-culture conditions. Two mechanisms by which endothelial cells influenced smooth muscle LDL metabolism were identified. First, mitogen(s) derived from endothelial cells stimulated entry of SMCs into the growth cycle, and the changes in LDL metabolism occurred as a consequence of G1-S transition. Second, SMC lipoprotein metabolism was stimulated in the absence of mitogens by a low molecular weight (<3,500) factor or factors. Co-culture was a required condition for the latter effect, suggesting that the mediator(s) may be unstable or that cell-cell communication was necessary for expression. These results (a) demonstrate that vascular cell interactions can modify LDL metabolism in SMCs, (b) provide some insights into the mechanisms responsible, and (c) identify co-culture as an experimental approach appropriate to certain aspects of vascular cell biology. (3)(4)(5) or by co-cultures in which endothelium and SMCs were plated together (3,6,7).In attempts to dissect such interactions, it is necessary to separate effects that may be mediated via cell-cell contact

show abstract

Section: Mechanisms Of Endothelial-specific Stimulation Of Ldl Metabolism In Smcsmentioning

confidence: 99%

Metabolic cooperation between vascular endothelial cells and smooth muscle cells in co-culture: changes in low density lipoprotein metabolism.

Davies¹,

Truskey²,

Warren³

et al. 1985

The Journal of Cell Biology

View full text Add to dashboard Cite

show abstract

“…48 The products of mevalonate metabolism are essential for SMC proliferation. 49 Statins decrease the rate of proliferation of SMCs in vitro and in vivo 50 and reduce metalloproteinase expression and secretion (see below), a pre-requisite for SMC-proliferation and migration. 51…”

Section: Smooth Muscle Cell Proliferationmentioning

confidence: 99%

The Role of Statins in Vascular Disease

Laws

Spark

Cowled

et al. 2004

European Journal of Vascular and Endovascular Surgery

View full text Add to dashboard Cite

show abstract

“…The effects of HMGCR inhibition have been extensively studied and the results demonstrated that, in addition to cholesterol, mevalonic acid or its non-sterol isoprenoid derivatives are also needed for cell division [113][114][115]. In human leukemia cells incubated in a medium free of cholesterol and mevalonic acid, the effects of statins on cell viability and cell cycle distribution greatly depend on the dose used.…”

Section: Role Of Cholesterol In Cell Proliferation and Differentiationmentioning

confidence: 99%

Role of cholesterol metabolism in the anticancer pharmacology of selective estrogen receptor modulators

Gómez-Coronado

Lasunción

Martı́nez-Botas

et al. 2021

Seminars in Cancer Biology

View full text Add to dashboard Cite

The dual role of mevalonate in the cell cycle.

Cited by 179 publications

References 23 publications

Metabolic cooperation between vascular endothelial cells and smooth muscle cells in co-culture: changes in low density lipoprotein metabolism.

Metabolic cooperation between vascular endothelial cells and smooth muscle cells in co-culture: changes in low density lipoprotein metabolism.

The Role of Statins in Vascular Disease

Role of cholesterol metabolism in the anticancer pharmacology of selective estrogen receptor modulators

Contact Info

Product

Resources

About