The Dual Functions of WLIM1a in Cell Elongation and Secondary Wall Formation in Developing Cotton Fibers

Han, Libo; Li, Yuanbao; Wang, Haiyun; Wu, Xiaomin; Li, Chunli; Luo, Ming; Wu, Shengyuan; Kong, Zhaosheng; Pei, Yan; Jiao, Guiai; Xia, Gui-Xian

doi:10.1105/tpc.113.116970

Cited by 157 publications

(138 citation statements)

References 70 publications

Supporting

Mentioning

129

Contrasting

Unclassified

Order By: Relevance

“…In our previous study, we found that WLIM1a could bind to filamentous actin (F-actin) and to a PAL-box DNA element (Han et al, 2013). Binding of WLIM1a to these two targets raised the question of whether these distinct functions of WLIM1a depend on different structural domains of the protein.…”

Section: Generation Of 6× His-tagged Recombinant Wlim1a and Wlim1a Vamentioning

confidence: 99%

“…In addition, they are key trans-acting factors regulating expression of genes involved in phenylpropanoid biosynthesis and cell division (Kawaoka et al, 2000;Thomas et al, 2006). Our previous work has shown that the cotton LIM domain protein, WLIM1a, has dual roles during fiber development (Han et al, 2013). WLIM1a may act as an actin bundler to promote cell elongation at the fiber-cell elongation stage or as a transcriptional factor to trigger lignin biosynthesis at the secondary cell wall biosynthesis stage.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, significant progress has been made in large-scale identification of genes and proteins involved in fiber development, particularly of those related to fiber elongation and secondary wall deposition (Arpat et al, 2004;Cao, 2015;Gou et al, 2007;Jin et al, 2013;Shi et al, 2006;Zhao et al, 2010). Moreover, a number of fiber development-related genes have been structurally or functionally characterized (Han et al, 2013;Li et al, 2005;Pei, 2015;Ruan et al, 2003;Shan et al, 2014;Xu et al, 2013;Zhang et al, 2011). These studies have made important progress towards understanding the molecular mechanisms governing cotton fiber development.…”

Section: Introductionmentioning

confidence: 99%

“…Compared with animal genomes that contain multiple LIM genes, plants genomes encode a limited number of LIM genes. Plant LIM proteins have been reported to function in either actin binding to promote formation of actin bundles or in DNA binding to regulate gene expression (Han et al, 2013;Moes et al, 2013;Zheng and Zhao, 2007). Tobacco WLIM1 and WLIM2 and six Arabidopsis LIM proteins are vital to bundle actin filaments and protect the actin cytoskeleton from disassembly (Dawid et al, 1995;Zheng and Zhao, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…WLIM1a may act as an actin bundler to promote cell elongation at the fiber-cell elongation stage or as a transcriptional factor to trigger lignin biosynthesis at the secondary cell wall biosynthesis stage. WLIM1a can bind both actin filaments and DNA, localizes in the cytosol and nucleus, and moves into the nucleus in response to hydrogen peroxide (Han et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

The two domains of cotton WLIM1a protein are functionally divergent

Han

Sun

et al. 2016

Sci. China Life Sci.

View full text Add to dashboard Cite

Our previous study demonstrated that WLIM1a has dual roles in fiber elongation and secondary cell wall synthesis in upland cotton, and the protein acts either as an actin-binding protein or as a transcription factor. Because WLIM1a consists of two different LIM domains, it is possible that these elements contribute differentially to the dual functions of the protein. In this study, we dissected the two LIM domains and characterized their biochemical functions. By using red fluorescent protein (RFP) fusion, co-sedimentation, and DNA binding methods, we found that the two domains of WLIM1a, domain1 (D1) and domain2 (D2), possessed different biochemical properties. While D1 contributed primarily to the actin filament-bundling activity of WLIM1a, D2 contributed to the DNA-binding activity of the protein; both D1 and D2 relied on a linker sequence for their activities. In addition, we found that WLIM1a and its two LIM domains form dimers in vitro. These results may lead to a better understanding of the molecular mechanisms of dual functions of WLIM1a during cotton fiber development. WLIM1a, actin bundle, transcription factor, dual functionCitation:

show abstract

Section: Generation Of 6× His-tagged Recombinant Wlim1a and Wlim1a Vamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The two domains of cotton WLIM1a protein are functionally divergent

Han

Sun

et al. 2016

Sci. China Life Sci.

View full text Add to dashboard Cite

show abstract

Phenylpropanoid Metabolism

Rock

2017

Encyclopedia of Life Sciences

View full text Add to dashboard Cite

Phenylpropanoid compounds encompass a wide range of structural classes with diverse biological functions in defence, survival and structural support associated with normal plant development (belying the term 'secondary metabolite'). The biosynthesis of phenylpropanoids is regulated by diverse environmental stimuli. Phenylpropanoid metabolism (other than flavonoids, not covered here) occupies a central place in the general aromatic metabolism of plants from shikimate to phenylalanine to lignin polymers and also to coumarins, phenolic volatiles and hydrolysable tannins. In recent years, genetics and biochemistry, along with methodology‐driven, computational, transgenic and comparative transcriptomic approaches, have led to significant advances in the identification of the families of genes encoding enzymes, transporters, regulatory factors involved in phenylpropanoid metabolism and a clearer picture of their functions in biotic and abiotic stress responses, plant development and enzyme/pathway evolution driven by interactions of species with their environment. Key Concepts Having one phenyl aromatic ring with one or more hydroxyl groups attached gives phenylpropanoids amphiphilic and reducing properties, underlying their ability to physically interact with other biomolecules. Plants invest a large percentage of their fixed carbon into synthesising phenylpropanoids, from simple volatile phenolic acids such as the defence hormone salicylic acid to complex polyphenolic flavonoids encompassing thousands of compounds with myriad physiological and adaptive functions. The shikimate pathway is the starting point for phenylpropanoid biosynthesis from shikimate product phenylalanine ( l ‐Phe), via the intermediate chorismate, which also serves as substrate for the synthesis of quinones and tocopherols important as electron acceptors in photosynthesis and aerobic respiration. Phenylpropanoid biosynthesis proceeds from deamination of Phe to cinnamate by the rate‐limiting and environmentally regulated enzyme PAL, followed by oxidation of the ring to p ‐coumarate, its activation with coenzymeA and a series of hydroxylation, methylation and reduction reactions to give cinnamic acids, ‐aldehydes and ‐alcohols that serve as substrates to generate a wide range of complex structures, notably polymers of coumaroyl, conferyl and sinapyl alcohols comprising lignin. Recent studies have established the major pathway in plants which proceeds from p ‐coumaroyl‐CoA → p ‐coumaryl‐shikimate → caffeoyl‐shikimate → caffeoyl‐CoA → feruloyl CoA → coniferaldehyde → 5‐OH coniferaldehyde → sinapaldehyde → sinapate/sinapyl alcohol (oxidation versus reduction, respectively), instead of the original model of a grid/matrix of parallel ring oxidation and side‐chain redox pathways from hydroxycinnamic acids to alcohols. Synthesis involves three subcellular compartments: chloroplast for Phe, cytosol for sinapoyl‐esters and the vacuole for trans‐esterifications. The identity of specific transporters, temporal‐ or organ‐specific regulatory factors for phenylpropanoid flux to (in)soluble polymers in the apoplast in response to biotic and abiotic stressors and whether lignin formation proceeds via precise channeling of individual precursors through metabolons (temporary structural–functional complexes formed between sequential enzymes) are key questions of practical significance that remain to be answered.

show abstract

Identification of quantitative trait loci for fiber quality, yield, and plant height traits in Upland cotton

et al. 2023

View full text Add to dashboard Cite

In cotton, most agronomic traits are controlled by polygenes. In this study, 110 F 6 recombinant inbred lines (RILs), derived from Upland cotton cross NC05AZ06 x NC11-2100, were used to develop a linkage map and to identify quantitative trait loci (QTL) for six fiber quality traits, three yield traits, and plant height. These RILs were genotyped using the CottonSNP63K array and phenotyped for fiber quality, yield traits, and plant height in 2-year field trials. Analysis of variance revealed significant (p < 0.05) differences among RILs for all traits studied, and the heritability estimates were moderate (30%-60%) to high (> 60%). Both positive and negative correlations were observed for fiber quality and yield traits. A total of 3,774 polymorphic SNP markers were used to develop a genetic map with an average marker density of 1.54 SNP/cM. Thirty QTL for fiber quality traits, yield traits, and plant height were detected on 15 different chromosomes, explaining 6.80%-20.02% of the phenotypic variance (PVE). Of these, 14 were major QTL (PVE > 10%), and three major QTL were detected in both years. Candidate gene analysis in the major QTL detected in both years and plant height QTL with PVE of 20.02% revealed five putative genes for fiber quality traits and one putative gene for plant height. The linkage map and identified QTL along with the candidate genes in the study could serve as additional breeding resources for Upland cotton genetic improvement.

show abstract

The Dual Functions of WLIM1a in Cell Elongation and Secondary Wall Formation in Developing Cotton Fibers

Cited by 157 publications

References 70 publications

The two domains of cotton WLIM1a protein are functionally divergent

The two domains of cotton WLIM1a protein are functionally divergent

Phenylpropanoid Metabolism

Identification of quantitative trait loci for fiber quality, yield, and plant height traits in Upland cotton

Contact Info

Product

Resources

About