The Drosophila ovary: an active stem cell community

Kirilly, Dániel; Xie, Ting

doi:10.1038/sj.cr.7310123

Cited by 188 publications

(155 citation statements)

References 78 publications

(126 reference statements)

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“…In the Drosophila ovary, a handful of somatic cells, including TF cells, CpCs, and ECs, form a functional niche to control GSC self-renewal and differentiation in the apical region of germaria [2,3]. It has been well documented that the BMP/Dpp signaling derived from niche CpCs locally suppresses GSC differentiation by silencing bam transcription in GSCs [7,10].…”

Section: Discussionmentioning

confidence: 99%

“…In this paradigm, an anterior germline stem cell (GSC) and a posterior cystoblast (CB) cell are produced in Drosophila ovaries [2,3] (Figure 1A). The inherent asymmetry of GSC division depends on a microenvironment (or called niche) maintained by signals secreted from three types of somatic stromal cells, including terminal filament (TF) cells, cap cells (CpCs), and escort cells (ECs, also called inner germarium sheath (IGS) cells) [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

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Ci antagonizes Hippo signaling in the somatic cells of the ovary to drive germline stem cell differentiation

Kan

Chen

et al. 2015

Cell Res

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ci antagonizes Hippo signaling in the somatic cells of the ovary to drive germline stem cell differentiation

Kan

Chen

et al. 2015

Cell Res

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“…The Drosophila ovary consists of ;14-16 ovarioles, which contain strings of developing egg chambers of progressive age with a germarium at the anterior tip ( Fig. 1E; Kirilly and Xie 2007). As a consequence of dLsd1 mutation, oogenesis arrests very early, with dLsd1 homozygous mutant ovaries consisting of germaria that do not bud off egg chambers.…”

Section: Lid Suppresses Phenotypes Associated With Dlsd1 Mutationmentioning

confidence: 99%

Functional antagonism between histone H3K4 demethylases in vivo

Stefano¹,

Walker²,

Burgio³

et al. 2011

Genes Dev.

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Dynamic regulation of histone modifications is critical during development, and aberrant activity of chromatinmodifying enzymes has been associated with diseases such as cancer. Histone demethylases have been shown to play a key role in eukaryotic gene transcription; however, little is known about how their activities are coordinated in vivo to regulate specific biological processes. In Drosophila, two enzymes, dLsd1 (Drosophila ortholog of lysine-specific demethylase 1) and Lid (little imaginal discs), demethylate histone H3 at Lys 4 (H3K4), a residue whose methylation is associated with actively transcribed genes. Our studies show that compound mutation of Lid and dLsd1 results in increased H3K4 methylation levels. However, unexpectedly, Lid mutations strongly suppress dLsd1 mutant phenotypes. Investigation of the basis for this antagonism revealed that Lid opposes the functions of dLsd1 and the histone methyltransferase Su(var)3-9 in promoting heterochromatin spreading at heterochromatin-euchromatin boundaries. Moreover, our data reveal a novel role for dLsd1 in Notch signaling in Drosophila, and a complex network of interactions between dLsd1, Lid, and Notch signaling at euchromatic genes. These findings illustrate the complexity of functional interplay between histone demethylases in vivo, providing insights into the epigenetic regulation of heterochromatin/euchromatin boundaries by Lid and dLsd1 and showing their involvement in Notch pathway-specific control of gene expression in euchromatin.

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“…In the Drosophila ovary, 2 or 3 GSCs are located at the tip of the germarium, where they are directly anchored to cap cells through E-cadherin-mediated cell adhesion (3). In addition, GSCs are also laterally wrapped around by escort stem cells (4).…”

mentioning

confidence: 99%

eIF4A controls germline stem cell self-renewal by directly inhibiting BAM function in the Drosophila ovary

Shen

Weng

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

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Stem cell self-renewal is controlled by concerted actions of extrinsic niche signals and intrinsic factors in a variety of systems. Drosophila ovarian germline stem cells (GSCs) have been one of the most productive systems for identifying the factors controlling self-renewal. The differentiation factor BAM is necessary and sufficient for GSC differentiation, but it still remains expressed in GSCs at low levels. However, it is unclear how its function is repressed in GSCs to maintain self-renewal. Here, we report the identification of the translation initiation factor eIF4A for its essential role in self-renewal by directly inactivating BAM function. eIF4A can physically interact with BAM in Drosophila S2 cells and yeast cells. eIF4A exhibits dosage-specific interactions with bam in the regulation of GSC differentiation. It is required intrinsically for controlling GSC self-renewal and proliferation but not survival. In addition, it is required for maintaining E-cadherin expression but not BMP signaling activity. Furthermore, BAM and BGCN together repress translation of Ecadherin through its 3 UTR in S2 cells. Therefore, we propose that BAM functions as a translation repressor by interfering with translation initiation and eIF4A maintains self-renewal by inhibiting BAM function and promoting E-cadherin expression.BGCN ͉ E-cadherin ͉ niche ͉ translation

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The Drosophila ovary: an active stem cell community

Cited by 188 publications

References 78 publications

Ci antagonizes Hippo signaling in the somatic cells of the ovary to drive germline stem cell differentiation

Ci antagonizes Hippo signaling in the somatic cells of the ovary to drive germline stem cell differentiation

Functional antagonism between histone H3K4 demethylases in vivo

eIF4A controls germline stem cell self-renewal by directly inhibiting BAM function in the Drosophila ovary

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