The Drosophila Microrna Bantam Regulates Excitability in Adult Mushroom Body Output Neurons to Promote Early Night Sleep

Hobin, Michael; Dorfman, Katherine; Adel, Mohamed; Rivera-Rodriguez, Emmanuel J.; Griffith, Leslie C.

doi:10.2139/ssrn.3942131

SSRN Journal

2021

DOI: 10.2139/ssrn.3942131

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The Drosophila Microrna Bantam Regulates Excitability in Adult Mushroom Body Output Neurons to Promote Early Night Sleep

Michael Hobin

Katherine Dorfman

Mohamed Adel

et al.

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2024

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“…They have been implicated in a plethora of physiological and pathological processes, such as neuronal development and neurodegeneration ( 15 ), as well as modifying neuronal excitability via the regulation of the expression of genes encoding ion-channel ( 16 ), cytoskeletal ( 17 ), and scaffolding ( 18 ), among others. Despite their known role as central transcriptional regulators in all molecular pathways studied, only a limited number of studies in zebrafish ( 19 ), mice ( 19 , 20 ), rats ( 21 – 24 ), and flies ( 25 – 27 ) have demonstrated their functional involvement in the regulation of sleep. Taken together, these observations suggest that miRNAs represent an additional, evolutionarily conserved, layer of gene regulation implicated in sleep processes.…”

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confidence: 99%

Cortical miR-709 links glutamatergic signaling to NREM sleep EEG slow waves in an activity-dependent manner

Kompotis,

Mang,

Hubbard

et al. 2024

Proc. Natl. Acad. Sci. U.S.A.

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MicroRNAs (miRNAs) are key post-transcriptional regulators of gene expression that have been implicated in a plethora of neuronal processes. Nevertheless, their role in regulating brain activity in the context of sleep has so far received little attention. To test their involvement, we deleted mature miRNAs in post-mitotic neurons at two developmental ages, i.e., in early adulthood using conditional Dicer knockout (cKO) mice and in adult mice using an inducible conditional Dicer cKO (icKO) line. In both models, electroencephalographic (EEG) activity was affected and the response to sleep deprivation (SD) altered; while the rapid-eye-movement sleep (REMS) rebound was compromised in both, the increase in EEG delta (1 to 4 Hz) power during non-REMS (NREMS) was smaller in cKO mice and larger in icKO mice compared to controls. We subsequently investigated the effects of SD on the forebrain miRNA transcriptome and found that the expression of 48 miRNAs was affected, and in particular that of the activity-dependent miR-709 . In vivo inhibition of miR-709 in the brain increased EEG power during NREMS in the slow-delta (0.75 to 1.75 Hz) range, particularly after periods of prolonged wakefulness. Transcriptome analysis of primary cortical neurons in vitro revealed that miR-709 regulates genes involved in glutamatergic neurotransmission. A subset of these genes was also affected in the cortices of sleep-deprived, miR-709 -inhibited mice. Our data implicate miRNAs in the regulation of EEG activity and indicate that miR-709 links neuronal activity during wakefulness to brain synchrony during sleep through the regulation of glutamatergic signaling.

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mentioning

confidence: 99%

Cortical miR-709 links glutamatergic signaling to NREM sleep EEG slow waves in an activity-dependent manner

Kompotis,

Mang,

Hubbard

et al. 2024

Proc. Natl. Acad. Sci. U.S.A.

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The Drosophila Microrna Bantam Regulates Excitability in Adult Mushroom Body Output Neurons to Promote Early Night Sleep

Cited by 1 publication

References 64 publications

Cortical miR-709 links glutamatergic signaling to NREM sleep EEG slow waves in an activity-dependent manner

Cortical miR-709 links glutamatergic signaling to NREM sleep EEG slow waves in an activity-dependent manner

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