The draft genome, transcriptome, and microbiome of Dermatophagoides farinae reveal a broad spectrum of dust mite allergens

Chan, Ting‐Fung; Ji, Kunmei; Yim, Aldrin Kay Yuen; Liu, Xiao Yu; Zhou, Jun; Li, Rui Qi; Yang, Kevin Yi; Li, Jing; Li, Meng; Law, Patrick Tik Wan; Wu, Yu Tzu; Cai, Zewei; Qin, Hao; Bao, Ying; Leung, R. C. K.; Ng, Patrick Kwok Shing; Zhang, Ju; Zhong, Xue; Ran, Pi Xin; Zhong, Nan; Liu, Zhi Gang; Tsui, Stephen Kwok‐Wing

doi:10.1016/j.jaci.2014.09.031

Cited by 171 publications

(183 citation statements)

References 44 publications

(47 reference statements)

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“…Randall et al [1] help explain this in an analysis of their assembly of the genomic sequence of Derma tophagoides pteronyssinus. The assembly, also reported by Waldron et al [2], in fact follows those for D. farinae [3] and the closely related Euroglyphus maynei [4], but for the first time attention is directed to the issues that are important for allergy research, going beyond being a tool for the verification of newly discovered allergens. The completeness of the coverage of a genome assembly is a critical starting point for all genomic projects so considerable attention is given to the sequencing metrics, which showed high indicators of success.…”

supporting

confidence: 57%

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Blueprint for the House Dust Mite

Thomas

2018

Int Arch Allergy Immunol

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supporting

confidence: 57%

“…It was similarly left unassigned by Chan et al [3] for D. farinae. The homologues include those of the newly described Der f 24-34 allergens that were mostly identified in proteomic screens in China and have yet to be assessed for their IgE binding by gravimetric estimates or by titrations compared to the serodominant group 1 and 2 allergens.…”

mentioning

confidence: 99%

Blueprint for the House Dust Mite

Thomas

2018

Int Arch Allergy Immunol

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“…As we also demonstrated that rDer p 23 could not bind to chitin structures present on the surface of mite fecal pellets, we speculate that the localization of Der p 23 into the peritrophic matrix of the mite could result from interactions with molecular partners other than chitin, or from the existence of oligomeric forms of the allergen exposing the appropriate 4-cysteine-based chitin-binding domains to interact with chitin. It must also be pointed out that the Der f 23 amino acid sequence was shown to contain a putative chitin binding domain with 5 cysteine residues; 4/5 cysteine residues are conserved in Der p 23 [22]. However, the chitin-binding properties of Der f 23 have yet to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

The House Dust Mite Major Allergen Der p 23 Displays O-Glycan-Independent IgE Reactivities but No Chitin-Binding Activity

Soh¹,

Mignon

Suratannon³

et al. 2015

Int Arch Allergy Immunol

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Background: The in-depth characterization of the recently identified house dust mite (HDM) major allergen Der p 23 requires the production of its recombinant counterpart because the natural allergen is poorly extractable from fecal pellets. This study aimed to provide a detailed physico-chemical characterization of recombinant Der p 23 (rDer p 23) as well as to investigate its IgE reactivity in a cohort of HDM-allergic patients from Thailand. Methods: Purified rDer p 23, secreted from recombinant Pichia pastoris, was characterized by mass spectrometry and circular dichroism analyses as well as for its chitin-binding activity. The IgE-binding frequency and allergenicity of Der p 23 were determined by ELISA and RBL-SX38 degranulation assays, respectively. Results: Purified intact rDer p 23 carried O-mannosylation and mainly adopted a random coil structure. Polyclonal antibodies to rDer p 23 can detect the corresponding natural allergen (nDer p 23) in aqueous fecal pellet extracts, suggesting that both forms of Der p 23 share common B-cell epitopes. Despite its homologies with chitin-binding proteins, both natural Der p 23 and rDer p 23 were unable to interact in vitro with chitin matrices. Of 222 Thai HDM-allergic patients tested, 54% displayed Der p 23-specific IgE responses. Finally, the allergenicity of rDer p 23 was confirmed by the degranulation of rat basophil leukemia cells. Conclusion: Our findings highlighted important levels of Der p 23 sensitizations in Thailand. Our study clearly suggested that rDer p 23 is likely more appropriate for HDM allergy component-resolved diagnosis than HDM extracts.

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“…Once the process of sensitization has begun with Der p 1, lower concentrations of Der p 2 may find a sufficiently proallergenic milieu to also achieve sensitization. Of course, this is mainly speculation, and the different biochemical functions of both allergens may play a role jointly or individually [23][24][25][26][27][28] . Der p 1 belongs to the papainlike cysteine protease family with cysteine protease activity [23,24] .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Role of IgE Responses to Der p 1 and Der p 2 in Chinese House Dust Mite-Allergic Patients

Wang

Gao

Zhou

et al. 2015

Int Arch Allergy Immunol

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Background: The role of specific IgE (sIgE) against Der p 1 and Der p 2 in Chinese patients with house dust mite (HDM) allergy has not yet been well investigated. Methods: Onehundred patients were enrolled, based on sensitization and doctor-diagnosed allergy to HDM. Questionnaires were administered to document demographic and clinical characteristics. Serum IgE reactivity to Dermatophagoides pteronyssinus (Dp) extract, Der p 1, Der p 2 and Der p 10 was measured by ImmunoCAP. Results: Almost all patients were sensitized to Der p 1 (95%) and Der p 2 (93%), with both allergens together being largely responsible for the total anti-HDM IgE response. No evidence for a significant role of Der p 10 was found. Overall, IgE responses to HDM and its 2 major allergens were higher in children than in adults in this cross-sectional study. With increasing age, IgE responses to Der p 2 become more important. A positive correlation was observed between the reaction of sIgE against Dp, Der p 1 and Der p 2 and the number of organs (including the eyes, nose, lungs and skin) that were affected in patients. Conclusions: In China, Der p 1 and Der p 2 are the dominant allergens in patients with HDM allergy. The relative importance of Der p 1 and Der p 2 changes with age, in favor of Der p 2. Overall, sIgE titers were positively associated with the number of organs affected.

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The draft genome, transcriptome, and microbiome of Dermatophagoides farinae reveal a broad spectrum of dust mite allergens

Cited by 171 publications

References 44 publications

Blueprint for the House Dust Mite

Blueprint for the House Dust Mite

The House Dust Mite Major Allergen Der p 23 Displays O-Glycan-Independent IgE Reactivities but No Chitin-Binding Activity

Evaluation of the Role of IgE Responses to Der p 1 and Der p 2 in Chinese House Dust Mite-Allergic Patients

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