Blueprint for the House Dust Mite

Thomas, Wayne R.

doi:10.1159/000485897

Cited by 4 publications

(6 citation statements)

References 30 publications

(44 reference statements)

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“…Extending the relevance of D . pteronyssinus proteomics to the environment is essential, as very little is known about which HDM components are present in inhalable air [10]. Proteomic analysis of 21 Der p 1 positive HD samples revealed the presence of 150 D .…”

Section: Resultsmentioning

confidence: 99%

“…Proteins present in HDM faeces are of particular importance as faecal particles are inhaled deep into the lungs, due to their small size [4, 8, 9]. Very little is known about which HDM components are present in house dust or inhalable air, and assessment is limited to allergens for which there are ELISAs [10]. Therefore, it is our belief that characterising D .…”

Section: Introductionmentioning

confidence: 99%

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Proteome and allergenome of the European house dust mite Dermatophagoides pteronyssinus

et al. 2019

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The European house dust mite Dermatophagoides pteronyssinus is of significant medical importance as it is a major elicitor of allergic illnesses. In this analysis we have undertaken comprehensive bioinformatic and proteomic examination of Dermatophagoides pteronyssinus airmid, identified 12,530 predicted proteins and validated the expression of 4,002 proteins. Examination of homology between predicted proteins and allergens from other species revealed as much as 2.6% of the D . pteronyssinus airmid proteins may cause an allergenic response. Many of the potential allergens have evidence for expression ( n = 259) and excretion ( n = 161) making them interesting targets for future allergen studies. Comparative proteomic analysis of mite body and spent growth medium facilitated qualitative assessment of mite group allergen localisation. Protein extracts from house dust contain a substantial number of uncharacterised D . pteronyssinus proteins in addition to known and putative allergens. Novel D . pteronyssinus proteins were identified to be highly abundant both in house dust and laboratory cultures and included numerous carbohydrate active enzymes that may be involved in cuticle remodelling, bacteriophagy or mycophagy. These data may have clinical applications in the development of allergen-specific immunotherapy that mimic natural exposure. Using a phylogenomic approach utilising a supermatrix and supertree methodologies we also show that D . pteronyssinus is more closely related to Euroglyphus maynei than Dermatophagoides farinae .

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Proteome and allergenome of the European house dust mite Dermatophagoides pteronyssinus

et al. 2019

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“…House dust mites (HDMs) are one of the most important worldwide causes of allergic diseases, including allergic asthma, allergic rhinitis, and atopic dermatitis. Common species of HDM, like Dermatophagoides pteronyssinus, Dermatophagoides farinae (D. farinae), and Blomia tropicalis (Calderon et al, 2015), produce allergenic proteins, i.e., that bind IgE from sera of >5% of patients with symptoms of HDM allergies (Caraballo 2017;Thomas, 2018). To date, 39 groups of mite allergens have been reported.…”

Section: Introductionmentioning

confidence: 99%

Identification of LincRNA from Dermatophagoides farinae (Acari: Pyroglyphidae) for Potential Allergen-Related Targets

Zhou¹,

Wu²,

Zhu³

et al. 2020

Genet. Mol. Biol.

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Long noncoding RNAs (lncRNAs), especially their important subclass of long intergenic noncoding RNAs (lincRNAs), have been identified in some insects. They play important roles in the regulation of biological processes, such as immune response or cell differentiation and as possible evolutionary precursors for protein coding genes. House dust mites (HDMs) are recognized as allergenic mites because allergens are found in their feces and bodies. Dermatophagoides farinae is one of the most important pyroglyphid mites because of its abundance in the household. To determine if lincRNAs can regulate allergen presentation in HDMs, we analyzed RNA-seq data for HDMs. We identified 11 lincRNAs that are related to mRNAs coding for allergens in HDMs. Using qRT-PCR, we amplified 10 lincRNAs and their putative target allergen-encoding mRNAs, confirming expression of these lincRNAs and allergen genes. The results suggest that lincRNAs might be involved in the regulation of allergen production in HDMs and might represent potential acaricidal candidates to inhibit mite allergen production.

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“…3 HDM, remains the most common major perennial allergen source. 4 It is one of the most common allergens, of size 0.1-0.5 mm, and lives in pillows, mattresses, blankets, carpets and other soft materials. 4 They are actually harmless, as they neither bite nor transmit disease.…”

Section: Introductionmentioning

confidence: 99%

“…4 It is one of the most common allergens, of size 0.1-0.5 mm, and lives in pillows, mattresses, blankets, carpets and other soft materials. 4 They are actually harmless, as they neither bite nor transmit disease. However, for children with predisposition to allergies, HDM droppings contain approximately 14 fully identified proteins such as cysteine, protease and chymotrypsin, which have the potential to induce atopic inflammatory responses.…”

Section: Introductionmentioning

confidence: 99%

Effect of scaling and root planing on level of immunoglobulin E and immunoglobulin G₄ in children with gingivitis and house-dust mite allergy: A pilot randomised controlled trial

et al. 2019

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Background and Objective: There is a pressing need for developing innovative strategies to prevent allergic diseases among children. As house-dust mite (HDM) allergy is often seen in children with gingivitis, strategies should be derived from a conceptual framework of allergen elimination and pathogen eradication; one such strategy is dental scaling and root planing (SRP) to remove dental plaque and periodontal pathogens. The study aimed to evaluate the beneficial effects of comprehensive 6-months dental SRP to reduce the level of immunoglobulin E (IgE) and immunoglobulin G4 (IgG4) in children with gingivitis and HDM allergy. IgE and IgG4, whose production is controlled mainly by Th-2 cells and B cells, are proven biomarkers for atopic inflammatory responses. Methods: The present study conducted a non-blinded randomised controlled trial with superiority design. A total of 10 subjects (age range 6–16 years) with gingivitis and positive skin-prick test to HDM from Pediatric Allergy Outpatient Clinic, Dr. Soetomo General Hospital were enrolled in the present study. Of the 10 subjects, only five received dental SRP. We further evaluated total serum IgE and IgG4 level before and 6 months after treatment. Results and Discussion: Subjects in the standard treatment group showed a slight decrease in the IgE level ([Formula: see text]) but no change in the IgG4 level ([Formula: see text]), while subjects in the intervention group showed a significant decrease in IgE ([Formula: see text]) and IgG4 levels ([Formula: see text]). Conclusion: The study results suggest that 6-month comprehensive dental scaling combined with root planing may help to reduce IgE and IgG4 levels in children with gingivitis and HDM allergy. Furthermore, untreated or undertreated gingivitis is often associated with worsening allergic manifestation and thus should be avoided. Trial Registration: ISRCTN31416107, retrospectively registered on 17 April 2018.

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Blueprint for the House Dust Mite

Cited by 4 publications

References 30 publications

Proteome and allergenome of the European house dust mite Dermatophagoides pteronyssinus

Proteome and allergenome of the European house dust mite Dermatophagoides pteronyssinus

Identification of LincRNA from Dermatophagoides farinae (Acari: Pyroglyphidae) for Potential Allergen-Related Targets

Effect of scaling and root planing on level of immunoglobulin E and immunoglobulin G₄ in children with gingivitis and house-dust mite allergy: A pilot randomised controlled trial

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Blueprint for the House Dust Mite

Cited by 4 publications

References 30 publications

Proteome and allergenome of the European house dust mite Dermatophagoides pteronyssinus

Proteome and allergenome of the European house dust mite Dermatophagoides pteronyssinus

Identification of LincRNA from Dermatophagoides farinae (Acari: Pyroglyphidae) for Potential Allergen-Related Targets

Effect of scaling and root planing on level of immunoglobulin E and immunoglobulin G4 in children with gingivitis and house-dust mite allergy: A pilot randomised controlled trial

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Effect of scaling and root planing on level of immunoglobulin E and immunoglobulin G₄ in children with gingivitis and house-dust mite allergy: A pilot randomised controlled trial