The DR4–DQ8 haplotype and a specific T cell receptor Vβ T cell subset are associated with absence of allergy to Can f 1

Abstract

The DR4-DQ8 haplotype and the TCR Vbeta5.1(+) CD4(+) T cells may be protective against allergy to Can f 1.

Association of HLA class II alleles with sensitization to cow dander Bos d 2, an important occupational allergen

“…It has also been suggested that HLA-DRB1*04 would be associated with sensitivity to ␤-lactoglobulin, a lipocalin allergen of cow's milk (Sakaguchi et al 2002). However, in contrast to our present results with Bos d 2, our previous data with the major dog allergen Can f 1 suggested that the HLA haplotype DRB1*04-DQB1*0302 would protect from allergy instead of predisposing to it (Kinnunen et al 2005). This may be explicable by differences observed between the allergenic molecules.…”

“…It is interesting that cow and rat-allergic subjects share the protective haplotype although the alleles predisposing to the specific hypersensitivities are different. The HLA-DR3 haplotype was not found to be protective in dog allergy (Kinnunen et al 2005). However, diminished expression of DR3 haplotype in dog-allergic subjects can not be excluded since subjects carrying DRB1*0301 and DQB1*0201 alleles were missing from the relatively small study population (Kinnunen et al 2005).…”

T Cell Epitope-Containing Peptides of the Major Dog Allergen Can f 1 as Candidates for Allergen Immunotherapy

“…As the reactivity of Can f 1-allergic and nonallergic subjects' PBMCs to Can f 1 was weak, to obtain specific T cell lines (22), two stimulation cycles with rCan f 1 were required. To identify the epitopes of the allergen, 13 Can f 1-specific T cell lines (from 13 subjects) and 10 clones (from 7 subjects) with a sustained expansion capacity were selected.…”

Potential of an altered peptide ligand of lipocalin allergen Bos d 2 for peptide immunotherapy

“…The different Vb subtypes expressed by the TCLs were analyzed with the IOTest Beta Mark kit (Immunotech, Marseille, France) as previously described. 21 TCR Vb expansions were considered significant when more than 10% of the cells expressed the particular Vb subtype and the level of expression was at least 2 times greater than the expression in PBMC. The TCLs were also stained with the MHC class II tetramers and anti-CD4-PE-Cy5 together with anti-Vb13.1-FITC.…”