Association of HLA class II alleles with sensitization to cow dander Bos d 2, an important occupational allergen

Kauppinen, Anu; Peräsaari, Juha; Taivainen, Antti; Kinnunen, Tuure; Saarelainen, Soili; Rytkönen‐Nissinen, Marja; Jeal, H.; Jones, Meinir; Virtanen, Tuomas

doi:10.1016/j.imbio.2011.08.012

Cited by 16 publications

(9 citation statements)

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“…The nonallergic subjects reported no medical history of atopy or allergic symptoms of any kind. HLA class II genotyping for the DQ and DR alleles of the subjects was performed in the Clinical Laboratory of Finnish Red Cross Blood Service (Helsinki, Finland [28]) or in the Immunogenetics Laboratory of University of Turku (Turku, Finland [29]) with PCR-SSO and PCR-SSP methods (Table S1). The distribution of the HLA class II alleles did not differ between allergic and healthy subjects (Fisher's exact test, p>0.05).…”

Section: Methodsmentioning

confidence: 99%

Human CD4+ T Cell Responses to the Dog Major Allergen Can f 1 and Its Human Homologue Tear Lipocalin Resemble Each Other

et al. 2014

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Lipocalin allergens form a notable group of proteins, as they contain most of the significant respiratory allergens from mammals. The basis for the allergenic capacity of allergens in the lipocalin family, that is, the development of T-helper type 2 immunity against them, is still unresolved. As immunogenicity has been proposed to be a decisive feature of allergens, the purpose of this work was to examine human CD4+ T cell responses to the major dog allergen Can f 1 and to compare them with those to its human homologue, tear lipocalin (TL). For this, specific T cell lines were induced in vitro from the peripheral blood mononuclear cells of Can f 1-allergic and healthy dog dust-exposed subjects with peptides containing the immunodominant T cell epitopes of Can f 1 and the corresponding TL peptides. We found that the frequency of Can f 1 and TL-specific T cells in both subject groups was low and close to each other, the difference being about two-fold. Importantly, we found that the proliferative responses of both Can f 1 and TL-specific T cell lines from allergic subjects were stronger than those from healthy subjects, but that the strength of the responses within the subject groups did not differ between these two antigens. Moreover, the phenotype of the Can f 1 and TL-specific T cell lines, determined by cytokine production and expression of cell surface markers, resembled each other. The HLA system appeared to have a minimal role in explaining the allergenicity of Can f 1, as the allergic and healthy subjects' HLA background did not differ, and HLA binding was very similar between Can f 1 and TL peptides. Along with existing data on lipocalin allergens, we conclude that strong antigenicity is not decisive for the allergenicity of Can f 1.

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Section: Methodsmentioning

confidence: 99%

Human CD4+ T Cell Responses to the Dog Major Allergen Can f 1 and Its Human Homologue Tear Lipocalin Resemble Each Other

et al. 2014

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“…The non‐atopic control subjects had horse riding as a hobby, and were therefore constantly exposed to horse allergens. Human leucocyte antigen (HLA) class II genotyping for the DQ and DR alleles of the subjects was performed in the Clinical Laboratory of the Finnish Red Cross Blood Service (Helsinki, Finland) or in the Immunogenetics Laboratory of the University of Turku (Turku, Finland) with PCR‐based lanthanide‐labelled sequence‐specific oligonucleotide hybridization (Supplementary material, Table S1). Signed informed consent was provided by all subjects participating in the study and the study was approved by the Ethics Committee of Kuopio University Hospital, permission # 182/99.…”

Section: Methodsmentioning

confidence: 99%

Differential CD4⁺T‐cell responses of allergic and non‐allergic subjects to the immunodominant epitope region of the horse major allergen Equ c 1

et al. 2013

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SummaryThe responses of allergen-specific CD4 + T cells of allergic and healthy individuals are still incompletely understood. Our objective was to investigate the functional and phenotypic properties of CD4 + T cells of horseallergic and healthy subjects specific to the immunodominant epitope region of the major horse allergen Equ c 1. Specific T-cell lines (TCLs) and clones were generated from peripheral blood mononuclear cells with Equ c 1 143-160 , the peptide containing the immunodominant epitope region of Equ c 1. The frequency, proliferative response, cytokine production and HLA restriction of the cells were examined. The frequency of Equ c 1-specific CD4 + T cells was low (approximately 1 per 10 6 CD4 + T cells) in both allergic and non-allergic subjects. The cells of allergic subjects had a stronger proliferative capacity than those of non-allergic subjects, and they predominantly emerged from the memory T-cell pool and expressed the T helper type 2 cytokine profile, whereas the cells of non-allergic subjects emerged from the naive T-cell pool and produced low levels of interferon-c and interleukin-10. T-cell response to Equ c 1 143-160 was restricted by several common HLA class II molecules from both DQ and DR loci. As the phenotypic and functional properties of Equ c 1-specific CD4 + T cells differ between allergic and non-allergic subjects, allergen-specific T cells appear to be tightly implicated in the development of diseased or healthy outcome. Restriction of the specific CD4 + T-cell response by multiple HLA alleles suggests that Equ c 1 143-160 is a promising candidate for peptide-based immunotherapy.

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“…Kusano et al [9 ]analyzed the association between HLA-DP5 and the Japanese cedar pollen allergen, Cry j 1. Associations between several DR and DQ alleles and the cow dander allergen, Bos d 2, have been reported [10]. The known HLA class II restrictions of allergens are reviewed by Jahn-Schmid et al [11].…”

Section: Introductionmentioning

confidence: 99%

Associations between Milk and Egg Allergens and the HLA-DRB1/DQ Polymorphism: A Bioinformatics Approach

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Doytchinova²

2016

Int Arch Allergy Immunol

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Background: Little is known about the associations between human leukocyte antigens (HLAs) and food allergies. Our aim was to analyze the associations between the HLA class II polymorphism and food allergy using bioinformatics. Methods: A two-step algorithm was developed which mimics the food allergen processing in the human body. In the first step, the allergen is digested by pepsin, trypsin and chymotrypsin. In the second step, the digested fragments bind to the most frequent 12 HLA-DRB1 and 5 HLA-DQ alleles, and the binding affinities are predicted. Results: The algorithm was applied to 13 well-known milk and egg allergens. The predicted HLA binders were compared to known T-cell and IgE epitopes originating from the same allergens, and 77% of them were found to overlap. We found that the peptides generated from milk allergens bind to DRB1*01:01, DQ7 and DQ8 but not to DRB1*03:01, DRB1*04:04, DRB1*12:01 and DRB1*15:01. The peptides generated from egg allergens bind to DRB1*01:01, DQ4, DQ7 and DQ8 but not to DRB1*03:01, DRB1*04:04 and DRB1*12:01. They bind to all the DQs studied. The alleles that bind to allergen peptides could be considered as susceptible to the particular allergy and the nonbinding alleles as protective. Conclusions: The alleles DRB1*01:01, DQ7 and DQ8 are considered as susceptible to cow's milk allergy and DRB1*03:01, DRB1*04:04, DRB1*12:01 and DRB1*15:01 as protective. The alleles DRB1*01:01, DQ4, DQ7 and DQ8 are considered as susceptible to egg allergy and DRB1*03:01, DRB1*04:04 and DRB1*12:01 as protective. Protective DQs against egg allergy were not revealed in this study.

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Association of HLA class II alleles with sensitization to cow dander Bos d 2, an important occupational allergen

Cited by 16 publications

References 28 publications

Human CD4+ T Cell Responses to the Dog Major Allergen Can f 1 and Its Human Homologue Tear Lipocalin Resemble Each Other

Human CD4+ T Cell Responses to the Dog Major Allergen Can f 1 and Its Human Homologue Tear Lipocalin Resemble Each Other

Differential CD4⁺T‐cell responses of allergic and non‐allergic subjects to the immunodominant epitope region of the horse major allergen Equ c 1

Associations between Milk and Egg Allergens and the HLA-DRB1/DQ Polymorphism: A Bioinformatics Approach

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Association of HLA class II alleles with sensitization to cow dander Bos d 2, an important occupational allergen

Cited by 16 publications

References 28 publications

Human CD4+ T Cell Responses to the Dog Major Allergen Can f 1 and Its Human Homologue Tear Lipocalin Resemble Each Other

Human CD4+ T Cell Responses to the Dog Major Allergen Can f 1 and Its Human Homologue Tear Lipocalin Resemble Each Other

Differential CD4+T‐cell responses of allergic and non‐allergic subjects to the immunodominant epitope region of the horse major allergen Equ c 1

Associations between Milk and Egg Allergens and the HLA-DRB1/DQ Polymorphism: A Bioinformatics Approach

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Product

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Differential CD4⁺T‐cell responses of allergic and non‐allergic subjects to the immunodominant epitope region of the horse major allergen Equ c 1