The distributions of HLA‐A, HLA‐B, HLA‐C, HLA‐DRB1 and HLA‐DQB1 allele and haplotype at high‐resolution level in Zhejiang Han population of China

Abstract

The distributions of HLA allele and haplotype are variable in different ethnic populations and the data for some populations have been published. However, the data on HLA-C and HLA-DQB1 loci and the haplotype of HLA-A, HLA-B, HLA-C, HLA-DRB1 and HLA-DQB1 loci at a high-resolution level are limited in Zhejiang Han population, China. In this study, the frequencies of the HLA-A, HLA-B, HLA-C, HLA-DRB1 and HLA-DQB1 loci and haplotypes were analysed among 3,548 volunteers from the Zhejiang Han population using polymerase chain reaction sequencing-based typing method. Totals of 51 HLA-A, 97 HLA-B, 45 HLA-C, 53 HLA-DRB1 and 27 HLA-DQB1 alleles were observed.The top three frequent alleles of HLA-A, HLA-B, HLA-C, HLA-DRB1 and HLA-DQB1 loci were Ahaplotypes with a frequency of ≥0.1% were found and the haplotypes with frequency greater than 3% were Ahood ratios test for the linkage disequilibrium of two loci haplotypes was revealed that the majority of the pairwise associations were statistically significant. The data presented in this study will be useful for searching unrelated HLA-matched donor, planning donor registry and for anthropology studies in China. K E Y W O R D Sallele frequency, human leucocyte antigen, polymerase chain reaction sequencing-based typing

The Polymorphism of HLA-A,-B,-C,-DRB1,-DQB1,-DRB3/4/5 loci in Zhejiang Han Population,China Using NGS Technology

“…In this study, the distribution of HLA-A, -B,-C, -DRB1, -DQB1 and -DRB3/4/5 loci in Zhejiang Han population atthree elds resolution level was reported. Our data showed thatthe characteristic of HLA allele in the Zhejiang Han population wasin close proximity to that of the Chinese southern Han populationat low and two elds resolution level, and consistent with ourprevious data [13][14][15]. However, comparison with the data at two elds resolution in our previous report, some HLA alleles werefurther divided.…”

“…The distributions of HLA alleles andhaplotypes among different populations and ethnic were various inthe previous reports. We have also reported the distribution ofHLA-A, HLA-B and HLA-DRB1 alleles with low resolution or two eldsresolution from Umbilical cord blood donors in Zhejiang province ofChina [13][14][15]. Here, the distributions of HLA-A, -B, -C,-DRB1, -DQB1, -DRB3/4/5 alleles and haplotypes at three eldresolution derived from umbilical cord blood donors in Zhejiangprovince were analyzed.…”

Identification of the novel HLA‐A*02 allele, HLA‐A*02:725

“…HLA‐A*02 is the most frequent allele with frequency of 30.35% in the Zhejiang Han population . He et al reported that HLA‐A*02:01 , A*02:03 , A*02:05 , A*02:06 , A*02:07 , A*02:10 were common alleles and 34 HLA‐A alleles were well‐documented alleles in the Chinese population .…”

Identification of the novel HLA‐B*40:357 allele by polymerase chain reaction sequence‐based typing

“…The HLA‐B locus is one of the highly polymorphic HLA loci and over 5881 HLA‐B alleles have been identified in populations around the world . It was reported that the top three most frequent alleles in HLA‐B locus were HLA‐B*40:01 , B*46:01 and B*58:01 with the frequency of 14.08%, 12.20% and 8.50%, respectively in the Zhejiang Han population, China . According to the data of the Chinese Marrow Donor Program Database (CMDP), the HLA‐B*40:01 allele is common in the Chinese Han population .…”

“…2 It was reported that the top three most frequent alleles in HLA-B locus were HLA-B*40:01, B*46:01 and B*58:01 with the frequency of 14.08%, 12.20% and 8.50%, respectively in the Zhejiang Han population, China. 3 According to the data of the Chinese Marrow Donor Program Database (CMDP), the HLA-B*40:01 allele is common in the Chinese Han population. 4 Here, we describe a novel allele HLA-B*40:357, which differs from HLA-B*40:01 by one nucleotide substitution.…”