The distribution of the Wnt5 protein in the tissues of the holothurian Eupentacta fraudatrix (Djakonov et Baranova, 1958) (Holothuroidea: Dendrochirotida) in the norm and during regeneration

Girich, Alexander S.; Dolmatov, Igor Yu.; Lamash, N. E.

doi:10.1134/s1063074014010052

Cited by 4 publications

(5 citation statements)

References 14 publications

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“…Transcriptome analysis of the AB and gut anlages has revealed transcripts of the wnt and frizzled genes . Moreover, the number of cells containing Wnt5 was shown to increase in regenerating tissues . These data indicate that regeneration of their internal organs in E. fraudatrix is regulated with the participation of Wnt signaling pathway.…”

Section: Introductionmentioning

confidence: 66%

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Wnt and frizzled expression during regeneration of internal organs in the holothurian Eupentacta fraudatrix

Girich

Isaeva

Dolmatov

2017

Wound Repair Regeneration

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Several genes of the Wnt and Frizzled families in the holothurian Eupentacta fraudatrix are characterized, and the complete coding sequences of wntA, wnt4, wnt6, wnt16, frizzled1/2/7, frizzled4, and frizzled5/8 are obtained. The dynamics of expression of these genes during regeneration of internal organs after evisceration are studied. Evisceration and the associated damages supposedly induce the expression of wnt16 on third day after evisceration. Genes wntA, wnt4, wnt6, and frizzled1/2/7 up-regulate during the period of active morphogenesis (5-7 days after evisceration) and might participate in regulation of tissue and organ formation. The signaling induced via Frizzled5/8 is could be necessary for formation of the anterior (ectodermal) part of the digestive system and development of the calcareous ring on 10th day after evisceration. Our data suggest that the Wnt signaling pathway plays a significant role in the regulation of regeneration of internal organs in holothurians.

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Section: Introductionmentioning

confidence: 66%

“…It is currently known that Frizzled4 is able to interact with Wnt5 . E. fraudatrix may also have a similar ligand‐receptor pair, because the number of cells containing Wnt5 in the AB anlage increased on the days 3–5 after evisceration . Wnt5, by acting via Frizzled4, activates wntA , wnt4 , wnt6 , and frizzled1/2/7 .…”

Section: Discussionmentioning

confidence: 99%

Wnt and frizzled expression during regeneration of internal organs in the holothurian Eupentacta fraudatrix

Girich

Isaeva

Dolmatov

2017

Wound Repair Regeneration

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“…Furthermore, the Wnt5 expression is revealed during the regeneration of the anterior structures in E. fraudatrix [ 211 ]. Unlike other ligands, Wnt5-positive cells were localized mainly in the connective tissue of the AB anlage and regenerating nerve cords.…”

Section: Regeneration After Anterior Eviscerationmentioning

confidence: 99%

Molecular Aspects of Regeneration Mechanisms in Holothurians

Dolmatov

2021

Genes

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Holothurians, or sea cucumbers, belong to the phylum Echinodermata. They show good regenerative abilities. The present review provides an analysis of available data on the molecular aspects of regeneration mechanisms in holothurians. The genes and signaling pathways activated during the asexual reproduction and the formation of the anterior and posterior parts of the body, as well as the molecular mechanisms that provide regeneration of the nervous and digestive systems, are considered here. Damage causes a strong stress response, the signs of which are recorded even at late regeneration stages. In holothurian tissues, the concentrations of reactive oxygen species and antioxidant enzymes increase. Furthermore, the cellular and humoral components of the immune system are activated. Extracellular matrix remodeling and Wnt signaling play a major role in the regeneration in holothurians. All available morphological and molecular data show that the dedifferentiation of specialized cells in the remnant of the organ and the epithelial morphogenesis constitute the basis of regeneration in holothurians. However, depending on the type of damage, the mechanisms of regeneration may differ significantly in the spatial organization of regeneration process, the involvement of different cell types, and the depth of reprogramming of their genome (dedifferentiation or transdifferentiation).

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“…There are three main species of holothurians that molecular mechanisms of regeneration are studied: Apostichopus japonicas (Sun, Yang, Chen, Ma, & Lin, 2013; Zhou et al, 2014), Eupentacta fraudatrix (Boyko et al, 2020) and Holothuria glaberrimma (Ortiz‐Pined et al, 2009; Rojas‐Cartagena et al, 2007). Various genes are being investigated in the repair of the aquapharyngeal bulb (AB), gut, and body wall (Dolmatov et al, 2021; Girich et al, 2014; Girich et al, 2017). Also, the larval transcriptome was analyzed in the process of recovery after transverse cutting in starfish (Cary et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Various genes are being investigated in the repair of the aquapharyngeal bulb (AB), gut, and body wall (Dolmatov et al, 2021;Girich et al, 2014;Girich et al, 2017). Also, the larval transcriptome was analyzed in the process of recovery after transverse cutting in starfish (Cary et al, 2019).…”

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confidence: 99%

WntA and Wnt4 during the regeneration of internal organs in the holothurian Eupentacta fraudatrix

Girich

2023

Genesis

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SummaryBackgroundOver the past few years, it has been established that wnt genes are involved in the regenerative processes of holothurians. The wnt4 gene was identified as one of the most active genes in Eupentacta fraudatrix regeneration using differential gene expression analysis and qPCR of individual genes. Also, the wntA gene was found in holothurians, which is present only in invertebrates and can perform unique functions.ResultsIn this regard, both these genes and proteins were studied in this work. During regeneration, the Wnt4 protein is found in the cells of the coelomic and ambulacral epithelium, retractor muscles, and radial nerves. Single cells with this protein are also found in the connective tissue of the developing aquapharyngeal bulb and in the hypoderm of the body wall. Cells with WntA are found exclusively in the hypoderm of the body wall.ConclusionWe assume that both genes are involved in regeneration, but Wnt4 coordinates the formation of the epithelial tissue structure, while WntA maintains the state of the intercellular substance of the body wall.

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The distribution of the Wnt5 protein in the tissues of the holothurian Eupentacta fraudatrix (Djakonov et Baranova, 1958) (Holothuroidea: Dendrochirotida) in the norm and during regeneration

Cited by 4 publications

References 14 publications

Wnt and frizzled expression during regeneration of internal organs in the holothurian Eupentacta fraudatrix

Wnt and frizzled expression during regeneration of internal organs in the holothurian Eupentacta fraudatrix

Molecular Aspects of Regeneration Mechanisms in Holothurians

WntA and Wnt4 during the regeneration of internal organs in the holothurian Eupentacta fraudatrix

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