The Distribution of Radioactive Phosphorus in the Electrophoretic Components of Egg Yolk Proteins ,

A Study of Turkey Egg Yolk 1. Composition and Electrophoretic Separation of Components

“…Chicken egg yolk proteins were resolved into at least 4 components by Clegg et al (1955), into 3 t o 4 components by Evans and Bandemer (1957), into 6 components by McCully et al (1962) and into 2 lipoproteins by Powrie et al (1963) on paper electrophoresis. Sugano (1955) separated chicken egg yolk proteins into 8 or 9 components in the Tiselius apparatus.…”

The Chemical Nature of Component I, a Phosphoprotein Isolated from the Blood Sera of Diethylstilbestrol-Treated Cockerels ,

“…Component I has been reported to be responsible for binding calcium in the blood sera of estrogenized cockerels and laying hens (Clegg et al, 1956;Ericson, 1955). Using a combined technique of moving boundary electrophoresis and P 32 tagging with a modified Tiselius cell, Clegg and Hein (1953) and Clegg et al (1955) observed that the P 32 activity associated with Component I was very high. Chemical characterization of Component I would therefore provide a correlation between its electrophoretic behavior and its structural features, and might indicate more clearly the role of phosphorus in Component I.…”

Egg Protein Determination, Separation of Egg Yolk Proteins by Paper Electrophoresis

“…Egg yolk proteins have not been studied as extensively as the proteins of egg white, because until recently there have been no indications of any such important biochemically active materials in egg yolk, and because these proteins are difficult to isolate. Some studies of egg yolk proteins have been made using the conventional movingboundary electrophoresis technique (3,14,20), but the data obtained were fragmentary and not very clear-cut. Evans and Bandemer (5) used paper electrophoresis for the separation of egg white proteins and their quantitative determination.…”