The distribution of nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) in the medulla oblongata, spinal cord, cranial and spinal nerves of frog, Microhyla ornata

Jadhao, Arun G.; Biswas, Saikat; Bhoyar, Rahul C.; Pinelli, Claudia

doi:10.1016/j.jchemneu.2017.02.009

Cited by 3 publications

(2 citation statements)

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“…N-d histochemistry reveals a unique population of neurons [59]. N-d neurons are distributed of in medulla oblongata in human [60,61], frog [62] and rat [63,64]. There are many neurotransmitters distributed in both sensory neurons in DRG and input perceive region in gracile nuclei [65][66][67].…”

Section: Relevant Histologic and Neuroanatomical Features Of The Dystmentioning

confidence: 99%

NADPH diaphorase neuronal dystrophy in gracile nucleus, cuneatus nucleus and spinal trigeminal nucleus in aged rat

Hou

Jia

et al. 2019

Preprint

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NADPH -diaphorase (N-d) activity is commonly used to identify NOS-ergic neurons. In our previous study, Nd positive neuritic dystrophy and spheroid termed aging-related N-d Body is discovered in the lumbosacral spinal cord in the normal aging rats. Histological studies also reveal that N-d positive neurodegenerative changes occur in the gracile nucleus. We re-examined N-d activity in gracile nucleus in aged rat. We found Nd positive neuritic dystrophy and spheroid also occurred in the cuneatus nucleus and spinal trigeminal nucleus. Besides regular coronal section, longitudinal oriented dystrophic neurites were detected in the sagittal and horizontal section in gracile nucleus and dorsal column. We fziurther examined the medullary oblongata with regular classical histology including Golgi staining, immunocytochemistry of NOS and phosphorylated tau protein, neuronal tracing method with wheat germ agglutinin conjugated alexa-fluor-488 through sciatic nerve, and spinal cord transection at thoracic level. Most of N-d positive neuritic dystrophy and spheroid did not showed colocalization with NOS or phosphorylated tau protein. Neuronal tracing and spinal cord transection revealed that N-d dystrophic neurites in gracile nucleus originated from terminal of sensory projection from spinal cord and peripheral somatic input. The results suggested that aging-related N-d dystrophy in the gracile nucleus was unique morphological feature. In conclusion, it was postulated that the N-d dystrophy as a morphological marker of aging degenerative damage in normal aged organisms.

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Section: Relevant Histologic and Neuroanatomical Features Of The Dystmentioning

confidence: 99%

NADPH diaphorase neuronal dystrophy in gracile nucleus, cuneatus nucleus and spinal trigeminal nucleus in aged rat

Hou

Jia

et al. 2019

Preprint

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“…Glutamate is the most predominant excitatory neurotransmitter involved in synaptic transmissionfrom primary sensory nerves to spinal dorsal horn neurons 3 . NOS immunocytochemistry of the tissues, in situ hybridization of NOS mRNA and reduced nicotinamide adenine dinucleotid phosphate diaphorase (NADPH-d) histochemistry have been used for defining of NO-producing neurons in the brain and spinal cord 4,5 . Also, it has been shown that NO is released in different areas of the brain and spinal cord 2 .…”

Section: Introductionmentioning

confidence: 99%

Role of nitric oxide in the regulation of pain after nociceptive stimuli in rat spinal cord

Mete

Vural²,

Cezayirli³

et al. 2018

Cukurova Medical Journal

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In this study, we examined the responses of the neurons in the lumbar segments of the spinal cord to nociceptive stimuli induced by formalin using c-fos immunohistochemistry and NADPH-d histochemistry Materials and Methods: Thirteen male, Wistar albino rats were used. The rats were divided into 3 groups. Group 1: pain group (n=5). Group 2: sham group (n=5). Group 3: control group (n=3). In group 1, 50 µl of 30% formalin solution was injected subcutaneously unilaterally on the dorsal surface of the right foot. In group 2, phosphate buffered saline was injected subcutaneously unilaterally on the dorsal surface of the right foot. In group 3, no treatment was given. For each group lumbar spinal cord (SC) samples were taken. Samples were stained with c-fos and NADPH-d histochemistry and cfos/NADPH-d double-labeling methods and evaluated under light microscope. Results: We noted that cells on spinal cord sections stained with c-fos, NADPH-d and both became prominent with nociceptive stimuli. There were significant increases in the numbers of c-fos (+), NADPH-d (+) and double-labeled cells in pain group comparison to sham group. In group 1, NADPH was significantly increased in the Laminae I-IV of the lumbar segments of the SC on both sides and laminae VIII, IX and X on the same side. Conclusion: The findings suggest that NO is associated with formalin-induced FOS-ir in the spinal cord, that it may play an important role in the regulation of pain and that the nociceptive function is related to increased nitric oxide synthase (+) neurons in the spinal cord. Amaç: C-fos indüksiyonunun, periferik inflamasyona karşı spinal kordda meydana gelen nöron yanıtının düzenlenmesinde önemli bir rol oynamaktadır. Sinir dokusunda NADPH-diaphorase (NADPH-d) reaksiyonu nitrik oksit sentaz (NOS) aktivitesinin iyi bir göstergesidir. Gereç ve Yöntem: On üç erkek Wistar albino sıçan kullanıldı. Sıçanlar 3 gruba ayrıldı. Grup 1: ağrı grubu (n = 5). Grup 2: şam grup (n = 5). Grup 3: kontrol grubu (n = 3). Grup 1'de, 50 μl% 30 formalin solüsyonu, sıçan sağ ayağın dorsal yüzeyi üzerine tek taraflı subkutanöz enjekte edildi. Grup 2'de PBS, sıçan sağ ayağın dorsal yüzeyinde tek taraflı subkutanöz enjekte edildi. Grup 3'de ise tedavi verilmedi. Her grupta lumbar spinal cord (SC) örnekleri alındı. Numuneler c-fos immünohistokimyası, NADPH-d histokimyası ve c-fos / NADPH-d ikili etiketleme yöntemleriyle histokimyasal olarak boyandı ve ışık mikroskopu altında değerlendirildi. Bulgular: Spinal kord kesitlerinde, hücrelerin c-fos, NADPH-d ile boyandığını ve her ikisinin de nosiseptif uyaranlarla belirginleştiğini saptadık. Ağrı grubunda, şam grubuna göre c-fos (+) (CF), NADPH-d (+) (NA) ve çiftetiketli (CN) hücre sayısında belirgin artış saptandı. Grup 1'de NADPH, spinal kord lomber segment kesitlerinin her iki yanındaki I-IV. laminalarında, aynı tarafta ise VIII, IX ve X laminalarında anlamlı olarak artmış halde bulundu. Sonuç: Bulgular, NO'nun spinal kordda, formaline bağlı FOS-ir ile ilişkili olduğunu, ağrının düzenlenmesinde önemli bir rol oynayabil...

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Distribution of NADPH-diaphorase reactivity in the central nervous system of the common toad ( Bufo bufo )

Pinelli

Jadhao

Scandurra

et al. 2018

Journal of Chemical Neuroanatomy

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The distribution of nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) in the medulla oblongata, spinal cord, cranial and spinal nerves of frog, Microhyla ornata

Cited by 3 publications

References 70 publications

NADPH diaphorase neuronal dystrophy in gracile nucleus, cuneatus nucleus and spinal trigeminal nucleus in aged rat

NADPH diaphorase neuronal dystrophy in gracile nucleus, cuneatus nucleus and spinal trigeminal nucleus in aged rat

Role of nitric oxide in the regulation of pain after nociceptive stimuli in rat spinal cord

Distribution of NADPH-diaphorase reactivity in the central nervous system of the common toad ( Bufo bufo )

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