The Distribution of 5‐hydroxytryptamine and Adenosinetriphosphate in Cytoplasmic Particles of the Dog's Small Intestine

Prusoff, William H.

doi:10.1111/j.1476-5381.1960.tb00274.x

Cited by 18 publications

(9 citation statements)

References 8 publications

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“…There are no satisfactory data on the chemical composition of the enterochromaffin granules, since no homogenous preparations have been available. Prusoff ( 1960) has suggested that ATP is concentrated in these granules and may be involved in 5-HT binding, as suggested for adrenal medullary granules by Hillarp et al (1955). However, Prusoff gave no morphological data on the purity of his granule fraction.…”

Section: ) Histamine Liberator Compound 48/80 Liberates 5-ht From Mamentioning

confidence: 75%

5‐Hydroxytryptamine Release from Enterochromaffin Granules in vitro

Penttilä¹,

Saris²

1968

Acta Physiologica Scandinavica

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PENTTILA, A. and N.-E. SARIS. 5-Hydroxytryptamine release from enterochromafin granules 5-Hydroxytryptamine (5-HT) release in uilro from cow and pig enterochromaffin granule preparations was studied. An accelerated 5-HT release from the granule fraction was caused by: elevation of temperature, freezing and thawing, dilution, increase of the p H of the incubation medium, addition of chlorprornazine, irniprarnine, mepyramine, segontin@, tyramine, trypsin, hydrocortisone and cortisone. I n various isotonic salt and buffer solutions the amine release was faster than that in isotonic sucrose solution. A slight 5-HT release was induced by Ca++ ions, Titriplex III@, Titriplex IV@ and histamine, and an almost complete release by CuCI,, distilled water and all the detergents studied. Monoamine oxidase inhibitors (in 0.15 M phosphate buffer, p H 6.9) and MgCll (in 0.3 M sucrose solutions, pH 6.9) were the only chemicals that retarded amine release. The following chemicals had no effect : ACTH, thyroxine, adrenaline, noradrenaline, dihydroxyphenylalanine, tryptophan, indoleacetic acid, 3-hydroxytyramine, acetylcholine or butyrylcholine with eserine, and guanethidine. The release of 5-HT from enterochromaffin granules resembles in some ways amine release from platelets, mast cells or granules carrying norepinephrine but differs in others. The special features of the enterochromaffin granules are discussed.

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Section: ) Histamine Liberator Compound 48/80 Liberates 5-ht From Mamentioning

confidence: 75%

5‐Hydroxytryptamine Release from Enterochromaffin Granules in vitro

Penttilä¹,

Saris²

1968

Acta Physiologica Scandinavica

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“…The subcellular distribution of 5-hydroxytryptamine in brain also differed from that in neoplastic mast cells (Hagen et al, 1959; and from that in the intestine (Prusoff, 1960). In each of these studies, 5-hydroxytryptamine was found primarily in particles as dense or denser than mitochondria; in brain 5-hydroxytryptamine was found in fractions less dense than mitochondria (Table 3; Fig.…”

Section: Discussionmentioning

confidence: 90%

The Subcellular Distribution of Histamine, Slow‐reacting Substance and 5‐hydroxytryptamine in the Brain of the Rat

Carlini¹,

Green²

1963

British Journal of Pharmacology and Chemotherapy

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Various extracts of brain were prepared with dilute and concentrated acid, acetone and n-butanol. When the extracts were applied to the guinea-pig isolated ileum, they produced a slow contraction which was not prevented by an antihistamine; in addition, the acid and acetone extracts inhibited the action of histamine. Histamine in extracts of brain was separated from other pharmacologically active substances by chromatography on a carboxylic-acid resin and estimated biologically. The bulk of this histamine was found in small particulate material, whereas slow-reacting substance was found in particulate material of greater density. 5-Hydroxytryptamine was in both the small and large particles. There was no relationship between the distribution of these substances and that of succinic dehydrogenase activity. The measurement of histamine in brain both by biological assay on the guinea-pig ileum and by chemical assay using a fluorimetric procedure gave mean values of 53 and 246 ng/g of wet tissue, respectively. The high values obtained by the chemical assay are attributable in part to substances other than histamine which become fluorescent after reaction with o-phthalaldehyde in this procedure.The regional distribution of histamine in brain is similar to that of 5-hydroxytryptamine and noradrenaline (Adam, 1961). The purpose of the present experiments was to see whether or not histamine occurs in particulate material of brain; this material contains most of the 5-hydroxytryptamine, acetylcholine (Whittaker, 1959) and noradrenaline (Chrugciel, 1960) in brain. Accordingly, the subcellular distribution of histamine has been compared with that of 5-hydroxytryptamine. In addition, the subcellular distribution in brain of a slow-reacting substance is described, together with some of the characteristics of the substance; this part of the work arose from difficulties encountered in the separation of histamine from the slow-reacting substances. Finally, the fluorimetric and biological methods for measuring histamine in brain are compared. A preliminary report of this work has appeared (Green,

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“…According to PRUSOFF (1960), the ratio of ATP and 5-HT in enterochromaffin cell granules was 1:2.6. The uptake and release of biogenic amines through the granular membranes could be supposed to occur in normal conditions.…”

Section: Murraymentioning

confidence: 99%

Effects of p-chlorophenylalanine on rabbit taste receptor cells.

Fujimoto

1982

Arch. Histol. Jap., Arch. Histol. Jpn

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Summary. By administration of p-chlorophenylalanine (PCPA), a synthetic inhibitor of 5-hydroxytryptamine (5-HT), to rabbits, granular vesicles in the taste receptor cells remarkably decreased their contents.The recovery of this core substance depletion occurred in specimens killed on the 15th day after injection of the drug and in animals injected with 5-hydroxytryptophan (5-HTP) after treatment with PCPA.Electron microscope observation suggests that granular vesicles in the receptor cells are 5-HT-storing particles and favors the view based on recent fluorescence histochemistry which indicates the presence of a biogenic monoamine, presumably 5-HT, in rabbit foliate taste bud cells.A considerable variation in the appearance of mammalian taste bud cells has been demonstrated by light-and electron microscopy. On this basis, we have classified rabbit taste bud cells into three types and designated them as type I (dark cells), type II (light cells), and type III (distinctly receptor cells) by distinct cytoplasmic features and the manner of cell to nerve contact (MURRAY et al., 1969;FUJIMoTo and MURRAY, 1970;MURRAY, 1973).The type III cells resemble many of the type II cells in general shape and electron density of the cytoplasm but fundamentally differ from all the type II cells in their afferent synaptic specializations to the intragemmal nerve fibers; these include increased density of the plasma membrane along the nerves and aggregations of vesicles against the density.These vesicles are of two types, one, an electron lucent type of synaptic vesicle, and the other, less frequent, but always containing densely staining core. The latter, designated as granular vesicles in this article, is uniformly spherical in form ranging from 60 to 160 mp in diameter and mostly found in the deeper part of the cytoplasm below the nucleus and around the synaptic regions, while dense secretory granules of the type I cells apparently derived from the Golgi complex always exist above the nucleus. The core substance of these granular vesicles is usually vaguely granular rather than homogeneous like the dense granules of the type I cells and separated from the limiting membrane by a very narrow electron lucent "peripheral halo."The occurrence of granular vesicles in the cytoplasm of the type III cells is one of the distinctive features which discriminate this cell type from the other ones. They 365

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The Distribution of 5‐hydroxytryptamine and Adenosinetriphosphate in Cytoplasmic Particles of the Dog's Small Intestine

Cited by 18 publications

References 8 publications

5‐Hydroxytryptamine Release from Enterochromaffin Granules in vitro

5‐Hydroxytryptamine Release from Enterochromaffin Granules in vitro

The Subcellular Distribution of Histamine, Slow‐reacting Substance and 5‐hydroxytryptamine in the Brain of the Rat

Effects of p-chlorophenylalanine on rabbit taste receptor cells.

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