The distribution and heterogeneity of mast cells in tongue from five different avian species

Abstract

This study was conducted with the aim of determining the morphology, distribution and heterogeneity of mast cells in the tongues of seagull (Larus fuscus), common buzzard (Buteo buteo), goose (Anser anser), white stork (Ciconia ciconia) and Gerze rooster. The study used five samples of tongue material from each of the healthy adult avian species. The samples were fixed in 10% neutral-buffered formalin (NBF) solution, then, after routine tissue follow-up, the samples blocked with paraplast. Cross-sections with 5-6 μm of thickness were stained with the 0.5% toluidine blue and alcian blue/safranin O (AB/SO). In all five avian species, it was found that the mast cells were in different sizes and round, oval or spindle-shaped based on their place of distribution. Mast cell numbers were determined in stained with toluidine blue, examined ×40 objectives in a 1 mm area. It was observed that mast cell density in subepithelial lamina propria and microscopic papilla was higher in the tongues of all species. Mast cell distribution and heterogeneity varied through the tongue, and there were more mast cells in the dorsal side of the tongue than the ventral side. The highest amount of mast cells was found in the tongue of the Gerze rooster among all five species. In the tongue cross-sections stained with the combined method of alcian blue/safranin O (AB/SO), the mast cells were stained as AB (+), SO (+) and AB/SO (+) (mixed).

Kuluçka sonrası farklı dönemlerde broyler ileumdaki mast hücrelerinin sayısı ve heterojenitesi

“…When granule-specific dyes like AB/SO are applied to mast cells, the cytoplasm of MMCs that react positively to alcian blue stains blue. CTMC, on the other hand, reacts positively to safranin and has a red-pink cytoplasm (2). It has been reported to be observed in the chick embryo lung from the 15th day of incubation for MMCs and the 18th day of incubation for CTMCs (27).…”

“…The gastrointestinal tract gradually establishes its mature structure and function throughout development from newborn to adult (1). A significant number of antigenic foreign substances come into close contact with the intestines (2). The intestinal mucosa is a protective barrier that allows nutrients to be selectively absorbed while preventing the entry of pathogens (3).…”

A Histochemical and Immunohistochemical Study on Glandula Mandibularis Mast Cells in Angora Goat

“…A large number of foreign antigenic substances come into contact with the digestive system. Mast cells are abundant in systems exposed to the outside world, and they are among the first cell groups to respond to pathogens and foreign body entry (Ertugrul et al 2018). According to researches, mast cells in the salivary glands play an essential role in salivary antigen detection, and they collaborate closely with other antigen-presenting cells, including dendritic cells (David et al 2010).…”

“…In studies of goats' lower respiratory tract (Kurtdede et al 2000), female reproductive organs (Karaca et al 2009), and urinary system (Ertuğrul and Kurtdede, 2017), mast cells were observed metachromatically stained with toluidine blue. According to studies conducted in the digestive system (Uslu and Yörük, 2008), tongue (Ertugrul et al 2018), and goat respiratory (Kurtdede et al 2000) and urinary systems (Ertuğrul and Kurtdede, 2017), mast cells were round, oval, or spindle-shaped, and the nuclei were located centrally or eccentrically. Our findings are consistent with previous research in terms of staining characteristics and morphological properties of mast cells.…”

Effect of probiotic on mast cell density and expression of tryptase, chymase, and TNF-α in the urinary bladder of rats with high cholesterol

“…The most distinctive morphological feature of mast cells is secretory granules found in their cytoplasms such as biogenic amines, proteoglycans, and cytokines. When mast cells are stained with granule-specific dyes such as alcian blue and safranin O, they are divided into three subgroups according to their histochemical differences: alcian blue (AB) (+), safranin O (SO) (+), and AB/SO (+) (mixed) (13). Mast cells have a large number of costimulatory molecules.…”