The Distribution and Common Amino Acid Polymorphisms of Human Papillomavirus (HPV)-31 Variants in 2700 Women from Northern China

Liu, Mengfei; He, Zhonghu; Xi, Longfu; Li, Jingjing; Liu, Fangfang; Liu, Ying; Pan, Yaqi; Ning, Tao; Guo, Chuanhai; Xu, Ruiping; Zhang, Lixin; Cai, Hong; Ke, Yang

doi:10.1371/journal.pone.0099141

Cited by 7 publications

(6 citation statements)

References 36 publications

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“…However, this differs from the rate (0.4%) reported by a meta-analysis of females with normal cytology from Asia (28), which was in accordance with an international study that reported that the prevalence of HPV-31 is lower in Asia (0.3%) compared with the global average (0.8%), particularly in European (2.3%) and Latin American (1.2%) females with normal cytology (29). However, the rate of HPV-31 prevalence in Sichuan, China, differed from the 0.52% previously reported in Northern Chinese females (30). Future studies regarding geographic variation and ethnic differences may be worthwhile.…”

Section: Variation Of E7 At Nucleotide Position ---------------------supporting

confidence: 88%

“…Chagas et al (35) examined five HPV-31 positive specimens from Brazil and reported that the proportions of A, B and C variants were 57.2, 5.7 and 37.1%, respectively (35). However, previous investigations have only observed variant lineages A (64.3%) and C (35.7%) in Southern China (30). As above, in the present study the variant lineage A of HPV-31 was the most commonly detected variant, followed by C. However, the present study also observed the presence of variant lineage B, which has not been previously reported from Southern China.…”

Section: Variation Of L1 At Nucleotide Position ---------------------mentioning

confidence: 99%

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Genetic variability in E5, E6, E7 and L1 genes of human papillomavirus type 31

Zhang

Wang

et al. 2018

Mol Med Report

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Human papillomavirus (HPV) type 31 is an important pathogenic subtype associated with cervical cancer. The aims of the present study were to analyze E5, E6, E7 and L1 gene mutations of HPV‑31 among females, and to elucidate the evolutionary associations between them. In total, 87 positive samples were collected. The E5, E6, E7 and L1 genes were amplified by polymerase chain reaction and sequenced. Subsequently, two phylogenetic trees were constructed from the nucleotide sequences of the E5, E6 and E7 and the L1 variants of HPV‑31. In total, 31 mutation sites of E5, E6 and E7 genes were identified, of which 16 were non‑synonymous. T4053A (F80I), C285T (H60Y), C520T (A138V) and A743G (K62E) were the most common non‑synonymous mutations. A total of 30 mutation sites of L1 genes were identified, of which four were non‑synonymous. The most common non‑synonymous mutations of L1 genes were A6350G (T29A) and C6372A (T36N). By phylogenetic analysis, A and C variants were most frequently detected, while B variants were less frequently detected in this population. The sequence variation data obtained in the present study provides a foundation for future research regarding HPV‑induced oncogenesis, and may prove valuable for developing diagnostic probes and in the design of HPV vaccines for targeted populations.

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Section: Variation Of E7 At Nucleotide Position ---------------------supporting

confidence: 88%

Section: Variation Of L1 At Nucleotide Position ---------------------mentioning

confidence: 99%

Genetic variability in E5, E6, E7 and L1 genes of human papillomavirus type 31

Zhang

Wang

et al. 2018

Mol Med Report

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“…Both cases of HPV 16 belonged to HPV 16 lineage A, the case of HPV 31 belonged to HPV 31 lineage A, and the HPV 58 case belonged to HPV 58 lineage A. The lineage/sublineage classification of the 3 HPV types in HNC is consistent with the previous reports of HPV lineage distribution data in cervical cancer within China, 23–30 indicating that the lineage/sublineage classification of the HNC causing HPV might be same with cervical cancer. Those data updated the lineage distribution data of HPV in HNC within China, which could be instrumental for designing HPV vaccines and HPV detection assays in China.…”

Section: Discussionsupporting

confidence: 88%

Prevalence of human papillomavirus in archival head and neck cancer in the eastern Inner Mongolian Autonomous Region, China

Enqi

et al. 2020

Tumori

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Objective: To investigate the prevalence of human papillomavirus (HPV) in archival head and neck cancer (HNC) collected from the Tong-Liao area, which is located in east Inner Mongolia, China. Methods: The presence of HPV in 54 HNCs and 25 benign biopsies was detected and the sequence variation of the E6 gene in HPV-positive samples was analyzed to determine their lineage/sublineage classification. Results: HPV was detected in only 4 out of 54 HNCs and no benign biopsies were found to be HPV-positive. After further p16INK4a immunostaining, only 3 cases of HNC were positive for both HPV and p16INK4a. Phylogenetic analysis of the isolated E6 gene shows that the HPV 16, HPV 31, and HPV 58 isolated in this study belong to lineage A. Conclusions: The prevalence of HPV in HNC from this area is very low. The lineage/sublineage classification of the 3 HPV types in HNC in this area is consistent with the previous reported data of HPV lineage distribution in cervical cancer within China.

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“…HPV E7 protein was divided into three main domains CR (conserved region)1, aa 1–15; CR2, aa 16–37; and CR3, aa 38–98, CR1 and CR2 mediate binding of viral oncoproteins to overlapping cellular proteins, including the product of retinoblastoma gene (pRB), cyclin A and cyclin E [ 32 , 33 ]. 21–29 associated with the binding of retinoblastoma (Rb) tumor suppressor, since E7 acts as the major immortalizing protein through Rb/E2F pathway, amino acid alternations in Rb binding domain may lead to the change of E7′s ability to immortalize cells among variants [ 34 ]. The CR3 domain contains two “Cys-X-X-Cys” sequences located at amino acid residues 58–61 and 91–94 participating in Zn binding, are the most important regions associated with the transforming ability of E7 protein [ 32 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

Genetic variability of human papillomavirus type 39 based on E6, E7 and L1 genes in Southwest China

Wang³

et al. 2021

Virol J

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Background Human papillomavirus type 39 associated with genital intraepithelial neoplasia and invasive cancers, has a high prevalence in Southwest China. HPV E6, E7 are two main papillomavirus oncoproteins, closely relate to the function of HPV immortalization, cell transformation, and carcinogenesis. L1 is the major capsid protein, can reflect the replication status of the virus in cells and the progression of cervical lesions. The purpose of this study is to reveal the prevalence of HPV 39 and the genetic polymorphisms of HPV39 based on E6, E7 and L1 gene in southwest China. Methods Cell samples were collected by cervical scraped for HPV detecting and typing, and HPV39 positive samples were selected out. Important E6, E7 and L1 genes of HPV39 were sequenced and analyzed for the study of HPV39 genetic polymorphisms. Phylogenetic trees were constructed by Maximum-likelihood and Kimura 2-parameters methods in Molecular Evolutionary Genetics Analysis version 6.0. The selection pressures of E6, E7 and L1 genes were estimated by Datamonkey web server. The secondary and three-dimensional structure of HPV39 E6, E7 proteins were created by sopma server and SWISS-MODEL software. Results 344 HPV39 positive samples were selected from 5718 HPV positive cell samples. Among HPV39 E6-E7 sequences, 20 single nucleotide mutations were detected, including 10 non-synonymous and 10 synonymous mutations; 26 single nucleotide mutations were detected in HPV39 L1 sequences, including 7 non-synonymous and 19 synonymous mutations respectively. 11 novel variants of HPV39 E6-E7 (5 in E6 and 6 in E7) and 14 novel variants of HPV39 L1 were identified in this study. A-branch was the most frequent HPV39 lineage in southwest China during our investigation. Selective pressure analysis showed that codon sites 26, 87, 151 in E6 and 75, 180, 222, 272, 284, 346, 356 in L1 were positively selected sites, as well as codon sites 45, 138, 309, 381 were negative selection sites in L1 gene, E7 has neither positive selection sites nor negative selection sites. A certain degree of secondary and three-dimensional structure dislocation was existed due to the non-synonymous mutations. Conclusions Amino acid substitution affected the secondary and three-dimensional structure of HPV39, and resulting in the differences of carcinogenic potential and biological functions as well as the immune response due to the antigen epitopes difference, the antigen epitopes with stronger adaptability in Southwest will be screened out based on the above research results for the later vaccine development. And gene polymorphism of HPV39 in Southwest China may improve the effectiveness of clinical test and vaccine design, specifically for women in Southwest China.

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The Distribution and Common Amino Acid Polymorphisms of Human Papillomavirus (HPV)-31 Variants in 2700 Women from Northern China

Cited by 7 publications

References 36 publications

Genetic variability in E5, E6, E7 and L1 genes of human papillomavirus type 31

Genetic variability in E5, E6, E7 and L1 genes of human papillomavirus type 31

Prevalence of human papillomavirus in archival head and neck cancer in the eastern Inner Mongolian Autonomous Region, China

Genetic variability of human papillomavirus type 39 based on E6, E7 and L1 genes in Southwest China

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