The distinct transcriptomes of fast-twitch and slow-twitch muscles in Mongolian horses

Bao, Tugeqin; Han, Haitang; Li, Bei; Zhao, Yiping; Bou, Gerelchimeg; Zhang, Xinzhuang; Du, Ming; Zhao, Ruoyang; Mongke, Togtokh; Laxima,; Ding, Wenqi; Jia, Zijie; Dugarjaviin, Manglai; Bai, Ding

doi:10.1016/j.cbd.2019.100649

Cited by 14 publications

(17 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, we also investigated the high functional divergence between PM and BF based on functional enrichment analysis. As expected, the functional analysis based on KEGG results showed that DEGs involved in energy metabolism, oxidative phosphorylation and cardiac muscle contraction were more enriched in PM, including some important genes ( LDHB , ATP2A2 , myosin-7 ( MYH7 ), MYH6 , TNNC1 , TPM3 and TNNI1 ) that are generally more highly expressed in slow-twitch skeletal muscle and cardiac muscle [ 18 , 33 ]. However, the expression of myosin-2 ( MYH2 ), myosin-1-like ( MYH1 ) and MYH4 , which are molecular markers of adult fast/type II myofibers [ 34 ], was significantly higher in BF, indicating that BF was more enriched with type II myofibers than PM and showed a higher glycolysis level.…”

Section: Discussionmentioning

confidence: 78%

See 1 more Smart Citation

Comparative Transcriptome Analysis of Slow-Twitch and Fast-Twitch Muscles in Dezhou Donkeys

Shi

et al. 2022

Genes

View full text Add to dashboard Cite

The skeletal muscle fiber profile is closely related to livestock meat quality. However, the molecular mechanisms determining muscle fiber types in donkeys are not completely understood. In this study, we selected the psoas major muscle (PM; mainly composed of oxidative-type muscle fibers) and biceps femoris muscle (BF; mainly composed of glycolytic-type muscle fibers) and systematically compared their mRNA and microRNA transcriptomes via RNA-seq. We identified a total of 2881 differentially expressed genes (DEGs) and 21 known differentially expressed miRNAs (DEmiRs). Furthermore, functional enrichment analysis showed that the DEGs were mainly involved in energy metabolism and actin cytoskeleton regulation. The glycolysis/gluconeogenesis pathway (including up-regulated genes such as PKM, LDHA, PGK1 and ALDOA) was more highly enriched in BF, whereas the oxidative phosphorylation pathway and cardiac muscle contraction (including down-regulated genes such as LDHB, ATP2A2, myosin-7 (MYH7), TNNC1, TPM3 and TNNI1) was more enriched in PM. Additionally, we identified several candidate miRNA–mRNA pairs that might regulate muscle fiber types using the integrated miRNA–mRNA analysis. Combined with the results of protein–protein interaction (PPI) analysis, some interesting DEGs (including ACTN3, TNNT3, TPM2, TNNC2, PKM, TNNC1 and TNNI1) might be potential candidate target genes involved in the miRNA-mediated regulation of the myofibril composition. This study is the first to indicate that DEmiRs, especially eca-miR-193a-5p and eca-miR-370, and potential candidate target genes that are mainly involved in actin binding (e.g., ACTN3, TNNT3 and TNNC1) and the glycolysis/gluconeogenesis pathways (e.g., PKM) might coregulate the myofibril composition in donkeys. This study may provide useful information for improving meat quality traits in Dezhou donkeys.

show abstract

Section: Discussionmentioning

confidence: 78%

“…Knowledge of the roles of miRNAs in controlling the donkey muscle fiber profile remains limited. In a recent report on horses, Bao et al demonstrated that miR-499 and miR-206 can regulate the expression of SOX6 , thereby affecting the performance of fast- and slow-twitch muscle fiber types [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis of Slow-Twitch and Fast-Twitch Muscles in Dezhou Donkeys

Shi

et al. 2022

Genes

View full text Add to dashboard Cite

show abstract

“…As an important breed in northern China, Mongolian horses have rich genomic diversity ( 21 ) and have a variety of excellent traits, such as adaptability, cold resistance, roughage resistance, disease resistance, and good stamina ( 22 , 23 ). Thoroughbred horses, a horse species bred in 17th-century Britain, were manually selected to meet the desired conditions of speed, temperament, and body size ( 24 ).…”

Section: Introductionmentioning

confidence: 99%

Variations in the fecal microbiota and their functions of Thoroughbred, Mongolian, and Hybrid horses

Luo

et al. 2022

Front. Vet. Sci.

View full text Add to dashboard Cite

The horse gut is colonized by a rich and complex microbial community that has important roles in horse physiology, metabolism, nutrition, and immune functions. Fewer across-breed variations in horse gut microbial diversity have been illustrated. In this article, the gut microbiota of Thoroughbred, Mongolian, and Hybrid horses [first filial generation (F1) of Mongolian (maternal) and Thoroughbred (paternal)] were studied by second-generation high-throughput sequencing technology. Differences in gut microbiota composition and function between breeds were determined using diversity and functional prediction analysis. The alpha diversity analysis showed that Thoroughbred horses had a more abundant and diverse gut microbiota, while the diversity of gut microbiota in Hybrid horses was intermediate between Thoroughbred and Mongolian horses. Subsequent cluster analysis showed that Hybrid horses have a microbiota composition more similar to Mongolian horses. LEfSe analysis revealed that the bacterial biomarkers for Thoroughbred horses at the family level were Prevotellaceae, Rikenellaceae, Fibrobacteraceae, p_251_o5, Lactobacillaceae, and uncultured_bacterium_o_WCHB1_41; the bacterial biomarker for Mongolian horses was Planococcaceae; and the bacterial biomarkers for Hybrid horses were Moraxellaceae, Enterobacteriaceae, and Ruminococcaceae. The functional prediction results indicated that the metabolic pathways differ significantly between the breeds. Regarding metabolism, the Hybrid horses had the lowest proportion of the carbohydrate metabolic pathways, while the energy metabolic pathway had the highest proportion. The abundance ratios of the remaining eight metabolic pathways in Hybrid horses were between Thoroughbred and Mongolian horses. In conclusion, the results of this study showed an association between horse breeds and gut microbiota.

show abstract

“…Previous studies have reported that lipids and glycogen are the two main energy donors utilized by muscle tissue to produce ATP ( Romijn et al, 1993 ). Lipid packs more energy per gram than any other fuel to support endurance swimming and also provides most of the ATP for muscle recovery ( Richards, Heigenhauser & Wood, 2002 ; Weber et al, 2016 ), while glycogen metabolism rapidly produces energy for quickly and forcefully muscle contraction during high-intensity activities ( Bao et al, 2020 ). The energy obtained from lipid or glycogen through the process of β -oxidation and glycolysis, respectively.…”

Section: Discussionmentioning

confidence: 99%

Expression profiles and transcript properties of fast-twitch and slow-twitch muscles in a deep-sea highly migratory fish, Pseudocaranx dentex

Wang

Yang

et al. 2022

PeerJ

View full text Add to dashboard Cite

Fast-twitch and slow-twitch muscles are the two principal skeletal muscle types in teleost with obvious differences in metabolic and contractile phenotypes. The molecular mechanisms that control and maintain the different muscle types remain unclear yet. Pseudocaranx dentex is a highly mobile active pelagic fish with distinctly differentiated fast-twitch and slow-twitch muscles. Meanwhile, P. dentex has become a potential target species for deep-sea aquaculture because of its considerable economic value. To elucidate the molecular characteristics in the two muscle types of P. dentex, we generated 122 million and 130 million clean reads from fast-twitch and slow-witch muscles using RNA-Seq, respectively. Comparative transcriptome analysis revealed that 2,862 genes were differentially expressed. According to GO and KEGG analysis, the differentially expressed genes (DEGs) were mainly enriched in energy metabolism and skeletal muscle structure related pathways. Difference in the expression levels of specific genes for glycolytic and lipolysis provided molecular evidence for the differences in energy metabolic pathway between fast-twitch and slow-twitch muscles of P. dentex. Numerous genes encoding key enzymes of mitochondrial oxidative phosphorylation pathway were significantly upregulated at the mRNA expression level suggested slow-twitch muscle had a higher oxidative phosphorylation to ensure more energy supply. Meanwhile, expression patterns of the main skeletal muscle developmental genes were characterized, and the expression signatures of Sox8, Myod1, Calpain-3, Myogenin, and five insulin-like growth factors indicated that more myogenic cells of fast-twitch muscle in the differentiating state. The analysis of important skeletal muscle structural genes showed that muscle type-specific expression of myosin, troponin and tropomyosin may lead to the phenotypic structure differentiation. RT-qPCR analysis of twelve DEGs showed a good correlation with the transcriptome data and confirmed the reliability of the results presented in the study. The large-scale transcriptomic data generated in this study provided an overall insight into the thorough gene expression profiles of skeletal muscle in a highly mobile active pelagic fish, which could be valuable for further studies on molecular mechanisms responsible for the diversity and function of skeletal muscle.

show abstract

The distinct transcriptomes of fast-twitch and slow-twitch muscles in Mongolian horses

Cited by 14 publications

References 57 publications

Comparative Transcriptome Analysis of Slow-Twitch and Fast-Twitch Muscles in Dezhou Donkeys

Comparative Transcriptome Analysis of Slow-Twitch and Fast-Twitch Muscles in Dezhou Donkeys

Variations in the fecal microbiota and their functions of Thoroughbred, Mongolian, and Hybrid horses

Expression profiles and transcript properties of fast-twitch and slow-twitch muscles in a deep-sea highly migratory fish, Pseudocaranx dentex

Contact Info

Product

Resources

About