The distinct response of phenanthrene enriched bacterial consortia to different PAHs and their degradation potential: a mangrove sediment microcosm study

Ahmad, Manzoor; Yang, Qingsong; Zhang, Yanying; Ling, Juan; Sajjad, Wasim; Qi, Shu‐Hua; Zhou, Wan; Zhang, Ying; Lin, Xiancheng; Zhang, Yuhang; Dong, Junde

doi:10.1016/j.jhazmat.2019.120863

Cited by 65 publications

(16 citation statements)

References 65 publications

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“…which involves the oxidation of PAH rings (Haritash and Kaushik, 2009). Gram negative bacterial community has been reported to be more efficient PAHs degraders (Ahmad et al., 2019). Several bacteria including Acinetobacter calcoaceticus, Alcaligenes denitrificans, Alcaligenes odorans, Arthrobacter polychromogenes, Bacillus thuringiensis, Burkholderia cepacia, Mycobacterium vanbaalenii, Mycobacterium flavescens, Pseudomonas aeruginosa, Pseudomonas putida, Sphingomonas paucimobilis, Stenotrophomonas maltophilia etc have been reported to efficiently degrade the PAHs (Liu et al., 2017).…”

Section: Introductionmentioning

confidence: 99%

Screening of polycyclic aromatic hydrocarbon degrading bacterial isolates from oil refinery wastewater and detection of conjugative plasmids in polycyclic aromatic hydrocarbon tolerant and multi-metal resistant bacteria

Khatoon

Malik

2019

Heliyon

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Wastewater were collected from the effluent channel in the vicinity of Mathura oil refinery, U.P. (India) and analysed for physicochemical characteristics, heavy metals as well as organic compounds including PAHs. The interaction of PAHs and heavy metals with various group of microorganisms revealed the viable count of aerobic heterotrophs, asymbiotic nitrogen fixers, actinomycetes and fungi were found to be 2.38 Â 10 6 , 1.89 Â 10 4 , 2.20 Â 10 4 CFU/mL and 8.76 Â 10 3 CFU/mL respectively. We have selected and screened 50 bacterial isolates for their resistance/tolerance to heavy metal and PAHs. Out of 25 multi-metal resistant isolates, 6 were able to tolerate PAHs at the concentration of 5000 μg/mL (50μg/disc) to naphthalene, anthracene, phenanthrene and pyrene. The PAH degradation efficiency of the isolates was assessed using spectrophotometer with 100 μg/mL of phenanthrene and observed different degree of degradation ranging from 34-66% after 96 h of incubation. One of the bacterial isolates KWB3 (identified as Enterobacter ludwigii by 16S rDNA sequencing) exhibited maximum degradation efficiency (66%) was further tested for phenanthrene degrading ability in the presence and absence of a co-substrate (glucose) in a mineral salt medium; and a number of metabolites were produced and detected by GC-MS which revealed the presence of benzocoumarin, phthalic acid, catechol and several low molecular weight compounds. The DNA derived from multi-metal and PAHs tolerant bacteria were PCR amplified using Inc specific primers and positive PCR products were obtained with oriT and trfA2 of the IncP group; indicates that these bacteria have gene-mobilizing capacity.

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Section: Introductionmentioning

confidence: 99%

Screening of polycyclic aromatic hydrocarbon degrading bacterial isolates from oil refinery wastewater and detection of conjugative plasmids in polycyclic aromatic hydrocarbon tolerant and multi-metal resistant bacteria

Khatoon

Malik

2019

Heliyon

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“…The abundance of Flavobacterium was higher at 0-4d and then suddenly decreased from day 6 to day 25 in CT, while it was extremely low in CK (0.03-0.18%). Previous research showed Flavobacterium had the potential to degrade PAHs (Ahmad et al, 2019). The microbial community structure of the CK was a small change in the early stage, but the inoculation had a signi cant effect on the microbial community.…”

Section: Microbial Performance Under Pahs Expose In Soilmentioning

confidence: 89%

Interaction with Endogenous Microorganisms, Comamonas testosteroni Enhanced the Degradation of Polycyclic Aromatic Hydrocarbon in Soil

Sun

Cui

et al. 2021

Preprint

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This study was to explore the functional role of Comamonas testosteroni (Ct) on soil indigenous microorganisms, and analyze the effect of Ct on PAHs degradation in PAH-contaminated soil. Results showed that inoculation of Ct could degrade naphthalene (Nap), phenanthrene (Phe), and benzo [α] pyrene (BaP) significantly. The degradation rate of Nap, Phe and BaP was 81.18%, 63.38% and 37.98% on day 25, respectively, suggesting that the low molecular weight of Nap and Phe were easier to be degraded by microorganisms than BaP. Network analysis showed that inoculation of Ct significantly increased the bacteria closely related to PAHs. Structural equation models confirmed Steroidobacter as functional bacteria could affect the degradation of Nap and BaP. Inoculated Ct could effectively enhance the synergy among indigenous bacteria to degrade PAHs. This would be helpful to understand the function of inoculated strains in PAH-contaminated soil and identify functional microorganisms of PAHs remediation.

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“…Phenanthrene is majorly catalyzed by dioxygenase (Gran-Scheuch et al, 2017;Ahmad et al, 2019;Jia et al, 2019) but the involvement of monooxygenases has been reported also. Bacterial degradation of phenanthrenes starts with the formation of isomers (9,10-; 1,2-; and 3,4-) but the cis-3,4-dihydrophenanthrene-3,4-diol isomer (Mishra et al, 2019;Yin et al, 2020) has proven to be the dominant (Fig.…”

Section: Metabolic Pathways Of Phenanthrenementioning

confidence: 99%

2.2-Diphenic Acid: A Reliable Biomarker Of Phenanthrene Biodegradation

Fenibo

2021

Preprint

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Phenanthrene is among the 16 priority pollutant and its mitigation in the environment has been a global concern. It serves as a model compound when it comes to biodgradation study of polyaromatic hydrocarbons (PAHs) because it has both the Bay- and K-region found in most PAH pollutants. Like other PAH pollutants, different means are available for its remediation in the environment, including microbial biodegradation. Diverse species of bacteria and fungi metabolize phenanthrenes as their sole source of carbon and energy. However, bacteria are more diverse in comparison to fungi. This has been shown in published pathways of phenanthrene biodegradation implicating various intermediary metabolites, including 2,2-diphenic acid, which is a downline metabolite of 9,10-dihydroxyphenanthrene. Though the 2,2-diphenic acid has been widely demonstrated to produce carbon (iv) oxide and linked to phthalate, only few has traced salicylic acid as its downstream molecule. 2,2-diphenic acid mounts equivalent position to 1-hydroxy-2-naphthoic acid, metabolite that ends the phenanthrene metabolic pathway. This is because they both produce phthalic acid and salicylic acid. As a product of bacteria and fungi during phenanthrene degradation, 2,2-diphenic acid can serve as a dependable biomarker of phenanthrene metabolism in a polluted habitat, where microbial community exist freely.

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The distinct response of phenanthrene enriched bacterial consortia to different PAHs and their degradation potential: a mangrove sediment microcosm study

Cited by 65 publications

References 65 publications

Screening of polycyclic aromatic hydrocarbon degrading bacterial isolates from oil refinery wastewater and detection of conjugative plasmids in polycyclic aromatic hydrocarbon tolerant and multi-metal resistant bacteria

Screening of polycyclic aromatic hydrocarbon degrading bacterial isolates from oil refinery wastewater and detection of conjugative plasmids in polycyclic aromatic hydrocarbon tolerant and multi-metal resistant bacteria

Interaction with Endogenous Microorganisms, Comamonas testosteroni Enhanced the Degradation of Polycyclic Aromatic Hydrocarbon in Soil

2.2-Diphenic Acid: A Reliable Biomarker Of Phenanthrene Biodegradation

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