The disruption of bacterial membrane integrity through ROS generation induced by nanohybrids of silver and clay

Su, Hong-Lin; Chou, Chang‐Pin; Hung, Da-Jen; Lin, Siou-Hong; Pao, I-Chuan; Lin, Jixing; Huang, Fang‐Liang; Dong, Rui‐Xuan; Lin, Jiang‐Jen

doi:10.1016/j.biomaterials.2009.07.030

Cited by 470 publications

(319 citation statements)

References 46 publications

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“…4k) indicated that there was no rupture of cells, and cell morphologies remained intact like corresponding controls (Fig. 4h, j), in turn, ruling out possible rupture of the cells by ROS (Su et al 2009). Transmission electron microscopic (TEM) observation of cells of model organism further corroborated FESEM results that there is no cellular rupture (an insert in Fig.…”

Section: Msns Synthesis and Physico-chemical Characterizationmentioning

confidence: 72%

Innovative biofilm inhibition and anti-microbial behavior of molybdenum sulfide nanostructures generated by microwave-assisted solvothermal route

et al. 2014

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The incessant use of antibiotics against infectious diseases has translated into a vicious circle of developing new antibiotic drug and its resistant strains in short period of time due to inherent nature of microorganisms to alter their genes. Many researchers have been trying to formulate inorganic nanoparticles-based antiseptics that may be linked to broad-spectrum activity and far lower propensity to induce microbial resistance than antibiotics. The way-out approaches in this direction are nanomaterials based (1) bactericidal and (2) bacteriostatic activities. We, herein, present hitherto unreported observations on microbial abatement using non-cytotoxic molybdenum disulfide nanostructures (MSNs) which are synthesized using microwave assisted solvothermal route. Inhibition of biofilm formation using MSNs is a unique feature of our study. Furthermore, this study evinces antimicrobial mechanism of MSNs by reactive oxygen species (ROS) dependent generation of superoxide anion radical via disruption of cellular functions.

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Section: Msns Synthesis and Physico-chemical Characterizationmentioning

confidence: 72%

Innovative biofilm inhibition and anti-microbial behavior of molybdenum sulfide nanostructures generated by microwave-assisted solvothermal route

et al. 2014

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“…Several studies observed a high level of ROS in cells treated with Ag NPs [30][31][32][33]. In these conditions, the cells endure a very high oxidative stress that leads to the cellular inactivation.…”

Section: Role Of Agmentioning

confidence: 99%

Antibacterial activity of silver nanoparticles: A surface science insight

2015

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Summary Silver nanoparticles constitute a very promising approach for the development of new antimicrobial systems. Nanoparticulate objects can bring significant improvements in the antibacterial activity of this element, through specific effect such as an adsorption at bacterial surfaces. However, the mechanism of action is essentially driven by the oxidative dissolution of the nanoparticles, as indicated by recent direct observations. The role of Ag + release in the action mechanism was also indirectly observed in numerous studies, and explains the sensitivity of the antimicrobial activity to the presence of some chemical species, notably halides and sulfides which form insoluble salts with Ag + . As such, surface properties of Ag nanoparticles have a crucial impact on their potency, as they influence both physical (aggregation, affinity for bacterial membrane, etc.) and chemical (dissolution, passivation, etc.) phenomena. Here, we review the main parameters that will affect the surface state of Ag NPs and their influence on antimicrobial efficacy. We also provide an analysis of several works on Ag NPs activity, observed through the scope of an oxidative Ag + release.

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“…17−22 Additional toxicity mechanisms include the association of AgNPs with bacterial membranes and consequent membrane damage, 23−25 the intracellular uptake of AgNPs (<10 nm), 26 and the release of reactive oxygen species that induce a stress response in bacterial cells. 27,28 To unravel the potential contribution of these toxicity pathways in the environment and, in particular, to evaluate whether the nonionic toxicity routes are relevant in such a context, it is essential to have a good understanding of AgNP persistence in the environment. To our knowledge, only three studies evaluated AgNP dissolution over a time course of several months.…”

Section: ■ Introductionmentioning

confidence: 99%

Silver Release from Silver Nanoparticles in Natural Waters

Dobiáš

Bernier‐Latmani

2013

Environ. Sci. Technol.

268

193

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Silver nanoparticles (AgNPs) are used increasingly in consumer products for their antimicrobial properties. This increased use raises ecological concern because of the release of AgNPs into the environment. Once released, zero-valent silver may be oxidized to Ag + and the cation liberated or it may persist as AgNPs. The chemical form of Ag has implications for its toxicity; it is therefore crucial to characterize the persistence of AgNPs to predict their ecotoxicological potential. In this study, we evaluated the release of Ag from AgNPs of various sizes exposed to river and lake water for up to 4 months. Several AgNP-capping agents were also considered: polyvinylpyrrolidone (PVP), tannic acid (Tan), and citric acid (Cit). We observed a striking difference between 5, 10, and 50 nm AgNPs, with the latter being more resistant to dissolution in oxic water on a mass basis. However, the difference decreased when Ag was surface-area-normalized, suggesting an important role of the surface area in determining Ag loss. We propose that rapid initial Ag + release was attributable to desorption of Ag + from nanoparticle surfaces. We also observed that PVP-and Tan-AgNPs are more prone to Ag + release than Cit-AgNPs. In addition, it is likely that oxidative dissolution also occurs but at a slower rate. This study clearly shows that small AgNPs (5 nm, PVP and Tan) dissolve rapidly and almost completely, while larger AgNPs (50 nm) have the potential to persist for an extended period of time and could serve as a continuous source of Ag ions.

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The disruption of bacterial membrane integrity through ROS generation induced by nanohybrids of silver and clay

Cited by 470 publications

References 46 publications

Innovative biofilm inhibition and anti-microbial behavior of molybdenum sulfide nanostructures generated by microwave-assisted solvothermal route

Innovative biofilm inhibition and anti-microbial behavior of molybdenum sulfide nanostructures generated by microwave-assisted solvothermal route

Antibacterial activity of silver nanoparticles: A surface science insight

Silver Release from Silver Nanoparticles in Natural Waters

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