The Diploid Drone of<i>Apis Cerana Japonica</i>and its Chromosomes

Hoshiba, Hidehiro; Okada, Ichiji; Kusanagi, Akio

doi:10.1080/00218839.1981.11100488

Cited by 19 publications

(6 citation statements)

References 11 publications

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“…In Louisiana, the average flight time of A mellifera drones is between 13.56 and 16.26 h, but they flew almost 2 h earlier early in the mating season (Taber, 1964 (Ruttner et al, 1972;Ruttner and Maul, 1983). Hoshiba et al (1981) Yoshida, 1990) were similar to those described in Germany (Ruttner, 1973;Koeniger et al, 1979), but for A cerana japonica they were different. This species congregates above the crown of tall trees which are visually distinct (Yoshida and Yamazaki, 1993).…”

Section: Methodsmentioning

confidence: 64%

The mating flight times of native Apis cerana japonica Radoszkowski and introduced Apis mellifera L in sympatric conditions

1994

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Section: Methodsmentioning

confidence: 64%

The mating flight times of native Apis cerana japonica Radoszkowski and introduced Apis mellifera L in sympatric conditions

1994

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“…mellifera are n = 16 and 2n = 32, respectively [31,32]. From 126 chromosome spreads, the range in chromosome number we observed was 6–133 (Figure 3).…”

Section: Resultsmentioning

confidence: 99%

A Cell Line Resource Derived from Honey Bee (Apis mellifera) Embryonic Tissues

2013

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A major hindrance to the study of honey bee pathogens or the effects of pesticides and nutritional deficiencies is the lack of controlled in vitro culture systems comprised of honey bee cells. Such systems are important to determine the impact of these stress factors on the developmental and cell biology of honey bees. We have developed a method incorporating established insect cell culture techniques that supports sustained growth of honey bee cells in vitro. We used honey bee eggs mid to late in their embryogenesis to establish primary cultures, as these eggs contain cells that are progressively dividing. Primary cultures were initiated in modified Leibovitz’s L15 medium and incubated at 32°C. Serial transfer of material from several primary cultures was maintained and has led to the isolation of young cell lines. A cell line (AmE-711) has been established that is composed mainly of fibroblast-type cells that form an adherent monolayer. Most cells in the line are diploid (2n = 32) and have the Apis mellifera karyotype as revealed by Giemsa stain. The partial sequence for the mitochondrial-encoded cytochrome c oxidase subunit I (Cox 1) gene in the cell line is identical to those from honey bee tissues and a consensus sequence for A. mellifera. The population doubling time is approximately 4 days. Importantly, the cell line is continuously subcultured every 10–14 days when split at a 1:3 ratio and is cryopreserved in liquid nitrogen. The cell culture system we have developed has potential application for studies aimed at honey bee development, genetics, pathogenesis, transgenesis, and toxicology.

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“…Diploid males of the related C. glomerata also produce diploid sperm (Zhou et al, 2006) but it has not been determined whether females of this species mate with diploid males and produce triploid offspring. With the exception of E. foraminatus (Cowan and Stahlhut, 2004), diploid males of all species that have been studied so far produce diploid sperm and triploid progeny, if any (for example, Hoshiba et al, 1981;Chauvin et al, 1987;Yamauchi et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Diploid males sire triploid daughters and sons in the parasitoid wasp Cotesia vestalis

et al. 2007

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In the Hymenoptera, males develop as haploids from unfertilized eggs and females develop as diploids from fertilized eggs. In species with complementary sex determination (CSD), however, diploid males develop from zygotes that are homozygous at a highly polymorphic sex locus or loci. We investigated mating behavior and reproduction of diploid males of the parasitoid wasp Cotesia vestalis (C. plutellae), for which we recently demonstrated CSD. We show that the behavior of diploid males of C. vestalis is similar to that of haploid males, when measured as the proportion of males that display wing fanning, and the proportion of males that mount a female. Approximately 29% of diploid males sired daughters, showing their ability to produce viable sperm that can fertilize eggs. Females mated to diploid males produced all-male offspring more frequently (71%) than females mated to haploid males (27%). Daughter-producing females that had mated to diploid males produced more male-biased sex ratios than females mated to haploid males. All daughters of diploid males were triploid and sterile. Three triploid sons were also found among the offspring of diploid males. It has been suggested that this scenario, that is, diploid males mating with females and constraining them to the production of haploid sons, has a large negative impact on population growth rate and secondary sex ratio. Selection for adaptations to reduce diploid male production in natural populations is therefore likely to be strong. We discuss different scenarios that may reduce the sex determination load in C. vestalis.

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The Diploid Drone ofApis Cerana Japonicaand its Chromosomes

Cited by 19 publications

References 11 publications

The mating flight times of native Apis cerana japonica Radoszkowski and introduced Apis mellifera L in sympatric conditions

The mating flight times of native Apis cerana japonica Radoszkowski and introduced Apis mellifera L in sympatric conditions

A Cell Line Resource Derived from Honey Bee (Apis mellifera) Embryonic Tissues

Diploid males sire triploid daughters and sons in the parasitoid wasp Cotesia vestalis

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