The Differential Proliferative Ability of Satellite Cells in Lantang and Landrace Pigs

Abstract

Here, for the first time, we evaluate the hypothesis that the proliferative abilities of satellite cells (SCs) isolated from Lantang (indigenous Chinese pigs) and Landrace pigs, which differ in muscle characteristics, are different. SCs were isolated from the longissimus dorsi muscle of neonatal Lantang and Landrace pigs. Proliferative ability was estimated by the count and proliferative activity of viable cells using a hemocytometer and MTT assay at different time points after seeding, respectively. Cell cycle information was detected by flow cytometry. Results showed that there was a greater (P<0.05) number of SCs in Lantang pigs compared with Landrace pigs after 72 h of culture. The percentage of cell population in S phase and G2/M phases in Lantang pigs were higher (P<0.05), while in G0/G1 phase was lower (P<0.05) in comparison with the Landrace pigs. The mRNA abundances of MyoD, Myf5, myogenin and Pax7 in SCs from Lantang pigs were higher (P<0.05), while those of myostatin, Smad3 and genes in the mammalian target of rapamycin (mTOR) pathway (with the exception of 4EBP1) were lower (P<0.05) than the Landrace pigs. Protein levels of MyoD, myogenin, myostatin, S6K, phosphorylated mTOR and phosphorylated eIF4E were consistent with the corresponding mRNA abundance. Collectively, these findings suggested that SCs in the two breeds present different proliferative abilities, and the proliferative potential of SCs in Lantang pigs is higher than in Landrace pigs.

Molecular and functional heterogeneity of early postnatal porcine satellite cell populations is associated with bioenergetic profile

“…In the juvenile pig, the SC pool has been shown to be a critical component of both hyperplastic and hypertrophic processes61128. However, established mixed (mass) cultures of pig SC and myoblasts do not reflect the marked functional heterogeneity that has been shown to exist in SC pools of other species375051.…”

“…In addition, in early postnatal muscle, a high number of slowly proliferating SC is present72526 generating fusion-competent myoblasts that contribute DNA to growing myofibers, thereby increasing their capacity for protein synthesis122728. However, some SC can also divide asymmetrically to give rise to both myonuclei and SC9111518.…”

“…A few studies2848 have shown that, in piglets from breeds with low and high growth performance or with low and high postnatal growth rate, the ability of SC to proliferate and differentiate is altered. However, these studies have been performed with mixed (mass) cultures of myogenic precursor cells/SC and myoblasts that do not reflect the marked heterogeneity between the various subpopulations of these cells.…”

Diminished satellite cell fusion and S6K1 expression in myotubes derived from skeletal muscle of low birth weight neonatal pigs

“…; Wang et al. ). Moreover, external stimuli like growth factors, hormones, and nutrients could also regulate proliferation and differentiation (Brown ).…”

Study on the Developmental Expression ofLbx1Gene inLongissimus Dorsiof Mashen and Large White Pigs

“…In addition, as a Chinese indigenous pig, the proliferative potential of satellite cells in Mashen pig was higher than in western commercial breed. (Wang et al, 2012). The percentage of activated satellite cells in Mashen pig could be greater than in Large White at the same stage.…”