The Differential Expression and Snp Analysis of the Ovoinhibitor Gene in the Ovaries of Laying Duck Breeds (Anas Platyrhynchos)

Wu, Yinan; Liang, Huamin; Zhang, H; Pi, Jinsong; Pan, Alexander; Shen, Junda; Pu, Yuejin; Du, J. P.

doi:10.1590/1806-9061-2017-0665

Cited by 9 publications

(9 citation statements)

References 12 publications

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“…Therefore, ESR2 may be a possible marker for selecting ducks for egg-laying performance. Several candidate genes such as GH, PRL, OIH, MTNR, FSHR, IGF, and DRD2 have as well been studied to have an association with egg-laying traits in ducks ( X. Wu et al, 2014 ; Xu et al, 2017 ; Ye et al, 2017 ; Feng et al, 2018 ; Wu et al, 2018 ; Bai et al, 2019 ) but none is known about polymorphism of ESR2 and association with egg-laying traits in ducks.…”

Section: Discussionmentioning

confidence: 99%

“…The detection of single nucleotide polymorphisms ( SNPs ) has helped identify novel genetic markers to more precisely select animals for enhanced egg-production performance. The identification of SNPs in candidate genes and the correlation with egg-laying traits in chickens, geese, and ducks is an important technique used to genetically improve animal selection and production ( Kang et al, 2012 ; Li et al, 2013 ; Wu et al, 2014 , 2018 ; Kulibaba, 2015 ; Alsiddig et al, 2017 ; Mohamed et al, 2017 ; Niu et al, 2017 ; Xu et al, 2017 ; Ye et al, 2017 ; Feng et al, 2018 ; Bai et al, 2019 )…”

Section: Introductionmentioning

confidence: 99%

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Polymorphism analysis and expression profile of the estrogen receptor 2 gene in Leizhou black duck

Asiamah

Liu

et al. 2022

Poultry Science

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Our previous study on the ovarian transcriptomic analysis in Leizhou black duck revealed that the ESR2 gene was involved in hormone regulation in reproduction and the estrogen signaling pathway related to reproductive performance was enriched. This suggested that ESR2 may have a functional role in the reproductive performance of the Leizhou black duck. Thus, this study aimed at evaluating the polymorphism of the ESR2 gene and its association with egg-laying traits and the distribution pattern of ESR2 mRNA in laying and non-laying Leizhou black ducks. In this study, genomic DNA was extracted from blood samples of 101 Leizhou black ducks to identify single nucleotide polymorphisms ( SNPs ) of the ESR2 gene to elucidate molecular markers highly associated with egg-laying traits. Four each of laying and non-laying Leizhou black ducks were selected to collect different tissues to analyze the ESR2 gene expression. A total of 23 SNPs were identified and association analysis of the single SNP sites showed that SNPs g.56805646 T>C and exon 3-20G>A were significantly ( P < 0.05) associated with egg weight. Ducks with CT and AG genotypes had significantly higher (P < 0.05) egg weights than their respective other genotypes. Haplotype association analysis of g.56805646 T>C and exon 3-20G>A showed that the haplotypes were significantly associated with egg weight. Higher egg weight was seen in individuals with H3H4 haplotypes. In the hypothalamus-pituitary-gonadal ( HPG ) axis, the results of qRT/PCR showed that ESR2 mRNA was significantly ( P < 0.05) expressed in the ovaries of both duck groups than in the hypothalamus and pituitary. In the oviduct, ESR2 was significantly ( P < 0.05) higher in the infundibulum and magnum of laying and non-laying ducks respectively. This study provides a molecular marker for selecting Leizhou black ducks for egg production. In addition, it offers theoretical knowledge for studying the related biological functions of the ESR2 gene at the cellular level.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polymorphism analysis and expression profile of the estrogen receptor 2 gene in Leizhou black duck

Asiamah

Liu

et al. 2022

Poultry Science

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“…have as well been studied to have an association with egg-laying traits in ducks (24,(28)(29)(30)(31)(32) but none is known about polymorphism of ESR2 and association with egg-laying traits in ducks.…”

Section: Association Analysis Between Esr2 Gene Polymorphism and Egg-laying Traitsmentioning

confidence: 99%

“…The detection of single nucleotide polymorphisms (SNPs) has helped with the identification of novel genetic markers to more precisely select animals for enhanced eggproduction performance. The identification of SNPs in candidate genes and the correlation with egg-laying traits in chickens, geese, and ducks is an important technique used to genetically improve animal selection and production (24,25,34,35,(26)(27)(28)(29)(30)(31)(32)(33) Leizhou black duck is a duck breed widely distributed in the Leizhou Peninsula in China which has characteristics such as strong adaptability, strong disease resistance, long egg peak duration, early egg age, rich trace elements in eggs, and coarse feeding tolerance (36). As a high-quality local duck population, genetic diversity is an excellent genetic material to improve meat and egg performance and environmental adaptability.…”

mentioning

confidence: 99%

Polymorphism Analysis and Expression Profile of the Estrogen Receptor 2 Gene in Leizhou Black Duck

Asiamah

Liu

et al. 2021

Preprint

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BackgroundOur previous study on the ovarian transcriptomic analysis in Leizhou black duck revealed that the ESR2 gene was involved in hormone regulation in reproduction and the estrogen signaling pathway related to reproductive performance was enriched. This suggested that ESR2 may have a functional role in the reproductive performance of the Leizhou black duck. Thus, this study aimed at evaluating the polymorphism of the ESR2 gene and its association with egg-laying traits and the distribution pattern of ESR2 mRNA in laying and non-laying Leizhou black ducks. MethodIn this study, genomic DNA was extracted from blood samples of 101 Leizhou black ducks to identify single nucleotide polymorphisms (SNPs) of the ESR2 gene to elucidate molecular markers highly associated with egg-laying traits. Four (4) each of laying and non-laying Leizhou black ducks were selected to collect different tissues to analyze the ESR2 gene expression. ResultsA total of 23 SNPs were identified and association analysis of the single SNP sites showed that SNPs g.56805646 T>C and exon 3-20G>A were significantly (P < 0.05) associated with egg weight. Ducks with CT and AG genotypes had significantly higher (P < 0.05) egg weights than their respective other genotypes. Haplotype association analysis of g.56805646 T>C and exon 320G>A showed that the haplotypes were significantly associated with egg weight where higher egg weight was seen in individuals with H3H4 haplotypes. In the hypothalamus-pituitary-gonadal (HPG) axis, the results of qRT/PCR showed that ESR2 mRNA was significantly (P < 0.05) expressed in the ovaries of both duck groups than in the hypothalamus and pituitary. In the oviduct, ESR2 was significantly (P < 0.05) higher in the infundibulum and magnum of laying and nonlaying ducks respectively. ConclusionThis study provides molecular marker for selecting Leizhou black ducks for egg production and provides theoretical knowledge for the study of the related biological functions of the ESR2 gene at the cellular level.

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“…A polymorphism in the growth hormone gene (GH) was significantly associated with egg number in Muscovy ducks (Wu et al, 2014a). A polymorphism in the ovoinhibitor gene (OIH), which is the main proteinase inhibitor in the egg white, was associated with age at first egg, weight at first egg, and egg number at 72 weeks of age (Wu et al, 2018a). Polymorphisms within the genes insulin-like growth factor 2 (IGF2) and dopamine receptor 2 (DRD2) genes were associated with egg production traits, and both genes are highly expressed in the ovary of Muscovy ducks (Ye et al, 2017).…”

Section: Studies Of Egg Production In Waterfowlmentioning

confidence: 99%

Genetics and genomics of egg production traits in poultry species

Wolc¹,

Arango²,

Fulton³

2020

Advances in Poultry Genetics and Genomics

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Introduction 2 Measurements of egg production: partial and total egg production 3 Measurements of egg production: oviposition time 4 Measurements of egg production: clutch traits 5 Measurements of egg production: egg production curves 6 Biological traits related to egg production 7 Studies of genetics and genomics of egg production in broilers 8 Studies of egg production in quail 9 Studies of egg production in turkeys 10 Studies of egg production in waterfowl 11 Conclusion 12 Future trends 13 Where to look for further information 14 References 14 References

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The Differential Expression and Snp Analysis of the Ovoinhibitor Gene in the Ovaries of Laying Duck Breeds (Anas Platyrhynchos)

Cited by 9 publications

References 12 publications

Polymorphism analysis and expression profile of the estrogen receptor 2 gene in Leizhou black duck

Polymorphism analysis and expression profile of the estrogen receptor 2 gene in Leizhou black duck

Polymorphism Analysis and Expression Profile of the Estrogen Receptor 2 Gene in Leizhou Black Duck

Genetics and genomics of egg production traits in poultry species

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