The differences in the number of fibroblasts and blood vessels after the topical and systemic administration of Lactobacillus casei Shirota probiotics for the treatment of traumatic ulcers in Wistar rats (Rattus norvegicus)

Abstract

Background and Aim: The use of drugs as a therapy for traumatic ulcers may lead to drug resistance and other side effects. Lactobacillus casei Shirota can affect the number of fibroblasts and blood vessels in wound healing. The aim of this study was to investigate the difference in the number of fibroblast cells and blood vessels after the topical and systemic administration of L. casei Shirota probiotics in Wistar rats with traumatic ulcer. Materials and Methods: Overall, 36 healthy male Wistar rats aged 2-3 months old and weighing 175-250 g in body weight were used as a sample. Traumatic ulcer was made on the labial fornix incisive inferior. The subject rats were divided into groups: (1) A control group over 3 days, (2) a group that used distilled water over 7 days, (3) a group that underwent topical treatment over 3 days, (4) a group that used probiotics administered topically over 7 days, (5) a group that underwent systemic treatment over 3 days, and (6) a group that took oral probiotics for the traumatic ulcers over 7 days. The number of fibroblasts and blood vessels was observed through a hematoxylin-eosin examination. Results: Based on the results of the study, a significant difference was observed in the number of fibroblasts (p=0.00) and blood vessels (p=0.018) in the 3-day topical group that underwent a 3-day systemic administration of probiotics compared with the number of fibroblast cells in the 7-day topical group and 7-day systemic group (p=0.00). Conclusion: Overall, significant differences were observed in the number of fibroblasts and blood vessels in Wistar rats with traumatic ulcer after undergoing the topical and systemic administration of L. casei Shirota probiotics.

Microbiome Modulation as a Therapeutic Approach in Chronic Skin Diseases

“…Kusumaningsih et al investigated the differences in the number of fibroblast cells and blood vessels after the administration of the probiotic L. casei shirota topically and systemically during the onset of the healing of traumatic ulcers in Wistar rats. The number of fibroblasts and new blood vessels were significantly higher in the two intervention groups in a comparison with the control group [106]. A further study investigated wounded New Zealand rabbits infected with S. aureus and treated with L. fermentum, which secrets gaseous NO.…”

Anti-inflammatory and wound healing properties of lactic acid bacteria and its peptides

“…Studies show that L. casei was able to heal ulcers on the skin of diabetic rats, thereby reducing the area of the skin (Majid et al 2016 ). L. casei shirota increased the number of fibroblasts which aided in accelerating the healing process of traumatic ulcers in rats (Kusumaningsih et al 2021 ). Various other strains of lactic acid bacteria with anti-inflammatory property are shown in Table 1 .…”

What Surgeon Should Know about Probiotics

“…Furthermore, probiotics, especially from the Lactobacillus spp. genus, as is Lactobacillus casei Shirota , L. brevis and L. plantarum , can stimulate the migration and proliferation of fibroblasts—which restore the wound area by providing collagen in the new extra-cellular matrix—and the formation of new blood vessels through the involvement of endothelial and perivascular cells [ 30 , 31 ]. Additionally, some bacteriocins lead to significant neo-vascularization and cell migration, aiding in forming a thick epithelial layer [ 32 ].…”

“…In an experimental study on rats subjected to oral mucosa trauma, the topical administration of probiotics for seven consecutive days produced the highest number of fibroblasts and blood vessels compared with the rats receiving them orally [ 31 ]. Fibroblasts and blood vessels play an important role in the proliferation phase, primarily to correct defects in the wound area by providing collagen to the new extracellular matrix [ 19 , 33 ].…”