The difference of cadmium accumulation between the indica and japonica subspecies and the mechanism of it

Zhou, Quan; Shao, Gaoneng; Zhang, Ying-Xing; Dong, Qing; Wang, Hong; Cheng, Shifeng; Zhang, Yingxin; Shen, Xihong

doi:10.1007/s10725-016-0229-0

Cited by 23 publications

(13 citation statements)

References 31 publications

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“…1 A). Similar to previous studies (Arao and Ae 2003 , Liu et al 2005 ; Pinson et al 2015 ; Zhou et al 2017 ), the results indicate that the mean grain Cd accumulation in japonica accessions is significantly lower than that in indica accessions ( p < 0.001) (Fig. 1 B).…”

Section: Discussionsupporting

confidence: 91%

“…It was reported that some indica cultivars and temperate japonica cultivars contained a loss-of-function allele of OsHMA3 , in which results in weakened vacuolar sequestration of Cd in the roots and an enhanced Cd accumulation, while many japonica cultivars contained a functional allele of OsHMA3 (Ueno et al 2010 ; Yan et al 2016 ). Similarly, a significant difference was observed in the expression levels of OsNRAMP1 between the indica and japonica subspecies, which may also explain the difference in Cd accumulation between these subspecies (Zhou et al 2017 ). Our findings, together with the results from previous studies, suggest that there may be a different genetic basis of Cd accumulation in grain between indica and japonica , and the genes/QTLs responsible for Cd transportation and accumulation may be subspecies-specific.…”

Section: Discussionmentioning

confidence: 94%

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Genome-wide association study and candidate gene analysis of rice cadmium accumulation in grain in a diverse rice collection

Zhao

Zhang

et al. 2018

Rice

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BackgroundCadmium (Cd) accumulation in rice followed by transfer to the food chain causes severe health problems in humans. Breeding of low Cd accumulation varieties is one of the most economical ways to solve the problem. However, information on the identity of rice germplasm with low Cd accumulation is limited, particularly in indica, and the genetic basis of Cd accumulation in rice is not well understood.ResultsScreening of 312 diverse rice accessions revealed that the grain Cd concentrations of these rice accessions ranged from 0.12 to 1.23 mg/kg, with 24 accessions less than 0.20 mg/kg. Three of the 24 accessions belong to indica. Japonica accumulated significantly less Cd than indica (p < 0.001), while tropical japonica accumulated significantly less Cd than temperate japonica (p < 0.01). GWAS in all accessions identified 14 QTLs for Cd accumulation, with 7 identified in indica and 7 identified in japonica subpopulations. No common QTL was identified between indica and japonica. The previously identified genes (OsHMA3, OsNRAMP1, and OsNRAMP5) from japonica were colocalized with QTLs identified in japonica instead of indica. Expression analysis of OsNRAMP2, the candidate gene of the novel QTL (qCd3–2) identified in the present study, demonstrated that OsNRAMP2 was mainly induced in the shoots of high Cd accumulation accessions after Cd treatment. Four amino acid differences were found in the open reading frame of OsNRAMP2 between high and low Cd accumulation accessions. The allele from low Cd accumulation accessions significantly increased the Cd sensitivity and accumulation in yeast. Subcellular localization analysis demonstrated OsNRAMP2 expressed in the tonoplast of rice protoplast.ConclusionThe results suggest that grain Cd concentrations are significantly different among subgroups, with Cd concentrations decreasing from indica to temperate japonica to tropical japonica. However, considerable variations exist within subgroups. The fact that no common QTL was identified between indica and japonica implies that there is a different genetic basis for determining Cd accumulation between indica and japonica, or that some QTLs for Cd accumulation in rice are subspecies-specific. Through further integrated analysis, it is speculated that OsNRAMP2 could be a novel functional gene associated with Cd accumulation in rice.Electronic supplementary materialThe online version of this article (10.1186/s12284-018-0254-x) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 94%

Genome-wide association study and candidate gene analysis of rice cadmium accumulation in grain in a diverse rice collection

Zhao

Zhang

et al. 2018

Rice

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“…Cd is absorbed by plant roots and accumulated in plant tissues, when grown slightly or moderately Cd-polluted soil, plant growth and development may not be substantially affected but the accumulated Cd can enter the food chain and cause harmful effects to human health. In recent years, many experiments have shown genotypic differences in Cd accumulation among rice varieties [8,35]. The Cd accumulations in indica subspecies were more and easier than the rice grain was correlated with the uptake of Cd by roots, and the root-to-shoot or shoot-to-grain translocation abilities.…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have been carried out on the molecular mechanisms of Cd accumulation in rice, and several genes involved in Cd translocation and accumulation have been identified [4,5]; for example, phytochelatin synthase genes (OsPCS1 and OsPCS2) [6], the natural resistance-associated macrophage protein (Nramp) family genes (OsNRAMP1 and OsNRAMP5) [7,8], heavy metal ATPase gene (OsHMA2) [9], and low-affinity ion transporter gene (OsLCT1) [10], the Fe transporters (OsIRT1 and OsIRT2) [11]. OsLCD gene was expressed in the phloem of vascular bundle and leaf in rice root which was involved in the Cd accumulation of rice [12]; OsLCT1 protein was a membrane protein, involved in the transport process of Cd from the cell to the outside world [3]; Ueno reported that heavy metal ATP enzyme (OsHMA3) in rice could decrease Cd transport to the shoot [10,13]; and OsCCX2 was reported as a node-expressed transporter participated in Cd accumulation in rice grain of rice [14].…”

Section: Introductionmentioning

confidence: 99%

A novel family of PLAC8 motif-containing/PCR genes mediates Cd tolerance and Cd accumulation in rice

et al. 2019

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Background: Cd is one of the highly toxic heavy metals to most organisms, including humans and plants, and Cd-contaminated rice from China has become a global food safety issue. The early prediction of OsPCR (the plant cadmium resistance protein) which contained a PLAC8 domain was related with the accumulation of Cd in rice. To further understand the biological function of the OsPCR genes on the Cd tolerance and Cd accumulation in rice, we used a low grain-Cd-accumulating rice (xiushui 11) and a high grain-Cd-accumulating rice (xiushui 110) varieties to analyze the relationship between the expression levels of the two most abundant expression genes (OsPCR1 and OsPCR3) and the Cd concentrations in different tissues at different growth periods during Cd stress, and transgenic experiments of OsPCR1 and OsPCR3 were carried out. Results: OsPCR1 and OsPCR3 were closely related with Cd accumulation. Overexpression of OsPCR1 and OsPCR3 could not only increase the Cd tolerance, but also decrease the Cd accumulation obviously in different parts of the transgenic rice plants (especially in the rice grains), while the RNAi expression plants showed the opposite results. Conclusions: These results indicate that OsPCR1 and OsPCR3 play critical roles in Cd accumulation in rice, which provides a theoretical basis for the safe production of rice.

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“…In rice, Arao and Ae (2003) investigated genotypic variation in grain Cd content, and identified three cultivars with the lowest Cd concentrations. Since then, many researchers have investigated the genotypic and environmental variation in Cd accumulation and distribution in rice and attempted to select low-Cd genotypes (Liu et al, 2005(Liu et al, , 2007He et al, 2006;Yu et al, 2006;Yan et al, 2010;Cao et al, 2014b;Pinson et al, 2015;Li et al, 2017;Zhou et al, 2017). In general, durum wheat tends to accumulate more Cd in grains than bread wheat (Stolt et al, 2003;Greger and Löfstedt, 2004).…”

Section: Genetic Differences and Screening For Low-cd Cereal Cultivarmentioning

confidence: 99%

Breeding for low cadmium accumulation cereals

Chen

2020

J. Zhejiang Univ. Sci. B

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Cadmium (Cd) is an element that is nonessential and extremely toxic to both plants and human beings. Soil contaminated with Cd has adverse impacts on crop yields and threatens human health via the food chain. Cultivation of low-Cd cultivars has been of particular interest and is one of the most cost-effective and promising approaches to minimize human dietary intake of Cd. Low-Cd crop cultivars should meet particular criteria, including acceptable yield and quality, and their edible parts should have Cd concentrations below maximum permissible concentrations for safe consumption, even when grown in Cd-contaminated soil. Several low-Cd cereal cultivars and genotypes have been developed worldwide through cultivar screening and conventional breeding. Molecular markers are powerful in facilitating the selection of low-Cd cereal cultivars. Modern molecular breeding technologies may have great potential in breeding programs for the development of low-Cd cultivars, especially when coupled with conventional breeding. In this review, we provide a synthesis of low-Cd cereal breeding.

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The difference of cadmium accumulation between the indica and japonica subspecies and the mechanism of it

Cited by 23 publications

References 31 publications

Genome-wide association study and candidate gene analysis of rice cadmium accumulation in grain in a diverse rice collection

Genome-wide association study and candidate gene analysis of rice cadmium accumulation in grain in a diverse rice collection

A novel family of PLAC8 motif-containing/PCR genes mediates Cd tolerance and Cd accumulation in rice

Breeding for low cadmium accumulation cereals

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