Breeding for low cadmium accumulation cereals

Chen, Qin; Wu, Feibo

doi:10.1631/jzus.b1900576

Cited by 17 publications

(7 citation statements)

References 165 publications

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“…Rice with unacceptable Cd content has become a serious food safety problem in many rice production regions due to contaminations caused by industrialization and inappropriate waste management [ 11 , 12 ]. The development of rice varieties with low grain Cd content is seen as an economic and long-term solution of this problem [ 13 , 14 , 15 ], since rice varieties show significant genetic variation in the ability to accumulate Cd [ 16 , 17 ]. Identification of varieties with high tolerance to Cd and low grain Cd content is feasible.…”

Section: Introductionmentioning

confidence: 99%

The Rice Cation/H+ Exchanger Family Involved in Cd Tolerance and Transport

Zou

Chen

Meng

et al. 2021

IJMS

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Cadmium (Cd), a heavy metal toxic to humans, easily accumulates in rice grains. Rice with unacceptable Cd content has become a serious food safety problem in many rice production regions due to contaminations by industrialization and inappropriate waste management. The development of rice varieties with low grain Cd content is seen as an economic and long-term solution of this problem. The cation/H+ exchanger (CAX) family has been shown to play important roles in Cd uptake, transport and accumulation in plants. Here, we report the characterization of the rice CAX family. The six rice CAX genes all have homologous genes in Arabidopsis thaliana. Phylogenetic analysis identified two subfamilies with three rice and three Arabidopsis thaliana genes in both of them. All rice CAX genes have trans-member structures. OsCAX1a and OsCAX1c were localized in the vacuolar while OsCAX4 were localized in the plasma membrane in rice cell. The consequences of qRT-PCR analysis showed that all the six genes strongly expressed in the leaves under the different Cd treatments. Their expression in roots increased in a Cd dose-dependent manner. GUS staining assay showed that all the six rice CAX genes strongly expressed in roots, whereas OsCAX1c and OsCAX4 also strongly expressed in rice leaves. The yeast (Saccharomyces cerevisiae) cells expressing OsCAX1a, OsCAX1c and OsCAX4 grew better than those expressing the vector control on SD-Gal medium containing CdCl2. OsCAX1a and OsCAX1c enhanced while OsCAX4 reduced Cd accumulation in yeast. No auto-inhibition was found for all the rice CAX genes. Therefore, OsCAX1a, OsCAX1c and OsCAX4 are likely to involve in Cd uptake and translocation in rice, which need to be further validated.

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Section: Introductionmentioning

confidence: 99%

The Rice Cation/H+ Exchanger Family Involved in Cd Tolerance and Transport

Zou

Chen

Meng

et al. 2021

IJMS

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“…Cultivation of low-Cd cultivars has been of particular interest and is one of the most cost-effective and promising approaches to minimize human dietary intake of Cd. Several low-Cd cereal cultivars and genotypes have been developed worldwide through cultivar screening and conventional breeding [20,21]. Since cultivars are cultivated under the same ecological and pedological conditions and are exposed to the same Cd concentration, the variability of Cd accumulation can be determined by intraspecific variations in the activity of genes responsible for the absorption and transport of Cd in the plant [22].…”

Section: Discussionmentioning

confidence: 99%

The influence of barley genotype and growing conditions on zinc and cadmium accumulation

Tovstik

Shupletsova

2022

BIO Web Conf.

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Zinc and cadmium accumulation of in plant tissues of barley of the line 999-93 and of its regenerant forms resulting from cell selection was assessed. The scheme of the experiment: 1) control; 2) acidic; 3) cadmium. We used the method of inversion voltammetry to assess the share of zinc and cadmium in plant samples. To assess the share of active and total forms of zinc and cadmium in soil samples collected from root rhizosphere we used the method of atomic absorption spectroscopy. Barley plants accumulated zinc from 0.2 to 79.7 mg∙kg-1 of dry phytomass. The genotype of plants has little influence on zinc accumulation, and plant organs participate in equal measure in zinc accumulation. As for the soil background with cadmium excess, there is a tendency to lessening zinc absorption in all the plant organs, and it is not connected with the plant’s genotype. Unlike zinc, cadmium gets accumulated mostly in the root system. In the control background the share of cadmium in roots was 0.2–0.4 mg∙kg-1; in the acidic one – 0.2–3.6 mg∙kg-1; in the cadmium one – 5.5–9.5 mg∙kg-1. In barley grain grown on soil with excess of cadmium we did not find any IPC excess of cadmium. On backgrounds of the same acidity, the more cadmium concentration grew, the less zinc concentration in grain was, mostly it concerns the original genotype, to a smaller degree it concerns the regenerant line on the selective medium with cadmium and aluminum. Coupling accumulation of zinc and cadmium took place mostly on acidic background, it was characteristic of barley with the original genotype and the regenerant selected in vitro as cadmium-resistant; on control background coupling accumulation is characteristic of aluminum-resistant regenerant. These regenerant genotypes had a tendency to eliminating cadmium and absorbing zinc.

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“…77,216,217 Cd accumulation is a typical quantitative trait controlled by several QTLs based on molecular markers and linkage maps, 226 and efforts have been made to develop innovative methods for tagging alleles/QTLs associated with low-Cd uptake in rice and wheat. 216 However, the mapping accuracy of these QTLs is not consistently high, as they are often located in large genomic regions due to different genetic backgrounds and limited mapping resources. Additionally, QTL mapping is a time-consuming and laborious process, which can have an impact on subsequent QTL fine mapping, cloning, and molecular breeding due to its lower resolution.…”

Section: Molecular Marker-assisted Selection (Mas) Of Cd-pscmentioning

confidence: 99%

“…With advancements in molecular biological techniques researching cultivar-dependent Cd accumulation mechanism, molecular markers may provide a powerful and hopeful solution for the selection of low-Cd cultivars, thereby expediting progress in breeding efforts for low-Cd cultivars. , The molecular biomarker strategies have already been designed to develop Cd-PSCs, spanning various crops such as cereals, vegetables, and others (Table ). , Traditional strategies have been applied to screen Cd-PSCs in Chinese leaf mustard, water spinach, pakchoi, celery, leafy lettuce, and amaranth, ,,,,, as well as in potatoes, soybean, sunflower, flaxseed, peanut, pea, radish, and carrot.…”

Section: Molecular-assisted Breeding Of Cd-pscsmentioning

confidence: 99%

“…These findings have contributed to the advancement of breeding low-Cd cultivars. However, there are still challenges in identifying effective molecular markers for Cd accumulation in Cd-PSC breeding. ,, Cd accumulation is a typical quantitative trait controlled by several QTLs based on molecular markers and linkage maps, and efforts have been made to develop innovative methods for tagging alleles/QTLs associated with low-Cd uptake in rice and wheat . However, the mapping accuracy of these QTLs is not consistently high, as they are often located in large genomic regions due to different genetic backgrounds and limited mapping resources.…”

Section: Molecular-assisted Breeding Of Cd-pscsmentioning

confidence: 99%

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Molecular-Assisted Breeding of Cadmium Pollution-Safe Cultivars

Ni,

Mubeen,

Leng

et al. 2023

J. Agric. Food Chem.

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Cadmium (Cd) contamination in edible agricultural products, especially in crops intended for consumption, has raised worldwide concerns regarding food safety. Breeding of Cd pollution-safe cultivars (Cd-PSCs) is an effective solution to preventing the entry of Cd into the food chain from contaminated agricultural soil. Molecular-assisted breeding methods, based on molecular mechanisms for cultivar-dependent Cd accumulation and bioinformatic tools, have been developed to accelerate and facilitate the breeding of Cd-PSCs. This review summarizes the recent progress in the research of the low Cd accumulation traits of Cd-PSCs in different crops. Furthermore, the application of molecular-assisted breeding methods, including transgenic approaches, genome editing, marker-assisted selection, whole genome-wide association analysis, and transcriptome, has been highlighted to outline the breeding of Cd-PSCs by identifying critical genes and molecular biomarkers. This review provides a comprehensive overview of the development of Cd-PSCs and the potential future for breeding Cd-PSC using modern molecular technologies.

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Breeding for low cadmium accumulation cereals

Cited by 17 publications

References 165 publications

The Rice Cation/H+ Exchanger Family Involved in Cd Tolerance and Transport

The Rice Cation/H+ Exchanger Family Involved in Cd Tolerance and Transport

The influence of barley genotype and growing conditions on zinc and cadmium accumulation

Molecular-Assisted Breeding of Cadmium Pollution-Safe Cultivars

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