The diagnostic value and pathogenetic role of lncRNA-ATB in patients with osteoarthritis

Dang, Xiliang; Lian, Liping; Wu, Datong

doi:10.1186/s11658-018-0118-9

Cited by 23 publications

(11 citation statements)

References 20 publications

(20 reference statements)

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“…The decrease of an index in a disease may lead to the occurrence of the disease due to the lack of the index. For instance, studies have indicated that the downregulation of lncRNA-D16366 is a biomarker for the diagnosis of hepatocellular carcinoma (43) and the downregulation of lncRNA activated by TGF-β in serum is a reliable diagnostic marker for OA (44). In the present study, the diagnostic value of LncRNA GAS5 was determined by ROC curve analysis.…”

Section: Discussionmentioning

confidence: 81%

Autophagy dysfunction may be involved in the pathogenesis of ankylosing spondylitis

et al. 2020

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The present study aimed to investigate the expression and significance of the mRNA of genes associated with autophagy and long non-coding RNA (lncRNA) GAS5 in peripheral blood mononuclear cells (PBMCs) of patients with ankylosing spondylitis (AS). The mRNA levels of microtubule-associated protein light chain 3 (LC3), Beclin1, autophagy-related gene (ATG)3, ATG5, ATG12, ATG 16 ligand 1 (ATG16L1) and lncRNA growth arrest-specific 5 (GAS5) in PBMCs from 60 patients with AS and 30 healthy controls (HC) were examined by reverse transcription-quantitative PCR. The correlations between the levels of LC3, Beclin1, ATG3, ATG5, ATG12 and ATG16L1 mRNA as well as lncRNA GAS5 levels with disease activity and laboratory parameters in patients with AS were determined by Spearman correlation analysis. In addition, the diagnostic value of lncRNA GAS5 for AS was explored through establishing a receiver operating characteristic (ROC) curve. The results indicated that, compared to the HCs, patients with AS had lower expression levels of LC3, ATG5, ATG12, ATG16L1 and lncRNA GAS5 in their PBMCs. Compared with those in patients with inactive AS, the levels of ATG5 and ATG12 were lower than those in patients with active AS. Of note, ATG5 and ATG12 mRNA levels were negatively correlated with disease activity indexes. lncRNA GAS5 was positively correlated with the expression of Beclin1, ATG3, ATG5, ATG12 and ATG16L1. The area under the ROC curve for the use of lncRNA GAS5 expression to diagnose AS was 0.808 with a 95% CI of 0.714-0.902. In conclusion, patients with AS had decreased expression of genes associated with autophagy and lncRNA GAS5. The extent of the reduction in ATG5 and ATG12 expression levels in patients with AS was correlated with the disease severity and activity. Furthermore, lncRNA GAS5 was a diagnostic indicator of AS.

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Section: Discussionmentioning

confidence: 81%

Autophagy dysfunction may be involved in the pathogenesis of ankylosing spondylitis

et al. 2020

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“…[33][34][35] LncRNAs were widely investigated for their regulation of orthopedic disorders such as osteoarthritis and osteoporosis. 21,36 However, limited attention has been paid to the effect of lncRNAs on SONFH in the studies of lncRNAs. HOTAIR inhibits osteogenic differentiation of BMSCs, and is high-expressed in SONFH tissues.…”

Section: Discussionmentioning

confidence: 99%

<p>Neohesperidin Ameliorates Steroid-Induced Osteonecrosis of the Femoral Head by Inhibiting the Histone Modification of lncRNA HOTAIR</p>

Yuan¹,

Zhang²,

Zhu³

et al. 2020

DDDT

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“…Numerous studies have examined the diagnostic value of various lncRNAs in different types of diseases (43)(44)(45)(46)(47). Studies have investigated the possibility of lncRNAs in fibroblast synoviocytes as biomarkers for RA (48).…”

Section: Discussionmentioning

confidence: 99%

Differential long non‑coding RNA expression profiles in the peripheral blood and CD4+ T cells of patients with active rheumatoid arthritis

Feng

et al. 2020

Exp Ther Med

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The human transcriptome is primarily composed of long non-coding RNAs (lncRNAs), which are key regulatory molecules of multiple biological processes. In the present study, the expression profiles of lncRNAs in the peripheral blood and CD4 + T cells of patients with active rheumatoid arthritis (RA) were determined. Based on the expression profiles, 493 lncRNAs and 374 mRNAs were identified to be differentially expressed in the peripheral blood of active RA patients and healthy donors. Further verification of lncRNAs was performed using reverse transcription-quantitative (RT-q) PCR analysis of peripheral blood from 5 healthy donors and 5 patients with active RA and 14 additional differentially expressed genes were identified. CD4 + T cells in peripheral blood from 12 patients with active RA and 8 healthy donors were isolated using magnetic beads and qPCR was used to assess differentially expressed lncRNAs. The results suggested that 7 lncRNAs were upregulated and 2 were downregulated. The results indicated that these 9 lncRNAs may be involved in the pathogenesis of RA. An increased ratio of Th17: T-regulatory (Treg) cells was also observed. It may be hypothesized that LncRNAs serve important roles in the differentiation of CD4 + T cells. Receiver operating characteristic curve analysis suggested that these 9 lncRNAs are of potential clinical diagnostic value for RA. Pearson correlation analysis indicated that the correlation coefficient between Ensembl transcript (ENST)00000569543 and complement C4 was 0.623 (P<0.05), and that between ENST00000420096 and anti-cyclic citrullinated peptide antibody or disease activity evaluation score, the correlation coefficient was 0.662 and 0.605, respectively (P<0.05 for each). In conclusion, the results of the present study suggest a possible role of lncRNAs in the differentiation of CD4 + T cells and the pathogenesis of RA, as well as the potential value as diagnostic biomarkers for active RA.

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The diagnostic value and pathogenetic role of lncRNA-ATB in patients with osteoarthritis

Cited by 23 publications

References 20 publications

Autophagy dysfunction may be involved in the pathogenesis of ankylosing spondylitis

Autophagy dysfunction may be involved in the pathogenesis of ankylosing spondylitis

<p>Neohesperidin Ameliorates Steroid-Induced Osteonecrosis of the Femoral Head by Inhibiting the Histone Modification of lncRNA HOTAIR</p>

Differential long non‑coding RNA expression profiles in the peripheral blood and CD4+ T cells of patients with active rheumatoid arthritis

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