&lt;p&gt;Neohesperidin Ameliorates Steroid-Induced Osteonecrosis of the Femoral Head by Inhibiting the Histone Modification of lncRNA HOTAIR&lt;/p&gt;

Yuan, Shuai; Zhang, Chuanxin; Zhu, Yi; Wang, Bo

doi:10.2147/dddt.s255276

Cited by 15 publications

(10 citation statements)

References 41 publications

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“…In alignment with this line of thought, the response of long bones to a high dose of HE in the absence of injury or external inflammatory triggers was positive, presenting as an increase in bone mass parameters. The encouraging effect of lower doses of HE systemically delivered in long bones is supported by evidence demonstrating that a diet containing HE 0.5% prevents androgen-deficiency-induced bone loss in male mice [53], inhibits bone loss in ovariectomized mice [23] and neohesperidin ameliorates steroid-induced osteonecrosis of the femoral head mice model [54], and suppresses osteoclast differentiation, bone resorption and ovariectomized-induced osteoporosis in mice [28]. Our results evaluating the effects of high HE concentration (500 mg/kg) on femur phenotype showed that systemic HE intake reduced the cortical bone porosity and prevented a trabecular bone volume decrease, which supports the idea that in our periodontal model, the complexity of the disease environment and the local inflammation were crucial factors to modify the cell responses to HE.…”

Section: Discussionmentioning

confidence: 98%

Systemic Dietary Hesperidin Modulation of Osteoclastogenesis, Bone Homeostasis and Periodontal Disease in Mice

Gonçalves

Musskopf

Rivera-Concepcion

et al. 2022

IJMS

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This study aimed to evaluate the effects of hesperidin (HE) on in vitro osteoclastogenesis and dietary supplem entation on mouse periodontal disease and femoral bone phenotype. RAW 264.7 cells were stimulated with RANKL in the presence or absence of HE (1, 100 or 500 µM) for 5 days, and evaluated by TRAP, TUNEL and Western Blot (WB) analyses. In vivo, C57BL/6 mice were given HE via oral gavage (125, 250 and 500 mg/kg) for 4 weeks. A sterile silk ligature was placed between the first and second right maxillary molars for 10 days and microcomputed tomography (μCT), histopathological and immunohistochemical evaluation were performed. Femoral bones subjected or not to dietary HE (500 mg/kg) for 6 and 12 weeks were evaluated using μCT. In vitro, HE 500 µM reduced formation of RANKL-stimulated TRAP-positive(+) multinucleated cells (500 µM) as well as c-Fos and NFATc1 protein expression (p < 0.05), markers of osteoclasts. In vivo, dietary HE 500 mg/kg increased the alveolar bone resorption in ligated teeth (p < 0.05) and resulted in a significant increase in TRAP+ cells (p < 0.05). Gingival inflammatory infiltrate was greater in the HE 500 mg/kg group even in the absence of ligature. In femurs, HE 500 mg/kg protected trabecular and cortical bone mass at 6 weeks of treatment. In conclusion, HE impaired in vitro osteoclastogenesis, but on the contrary, oral administration of a high concentration of dietary HE increased osteoclast numbers and promoted inflammation-induced alveolar bone loss. However, HE at 500 mg/kg can promote a bone-sparing effect on skeletal bone under physiological conditions.

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Section: Discussionmentioning

confidence: 98%

Systemic Dietary Hesperidin Modulation of Osteoclastogenesis, Bone Homeostasis and Periodontal Disease in Mice

Gonçalves

Musskopf

Rivera-Concepcion

et al. 2022

IJMS

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“…[11][12][13] There have been increasing numbers of clinical studies on SONFH prevention and treatment with TCM, and the efficacy is significant. [14][15][16] It has been found clinically that TCM can achieve good results in SONFH treatment, and the results of a large number of animal experimental studies have shown that enhanced TCM use has good effects in SONFH treatment. [17,18] There remains a lack of relevant systematic reviews in clinical practice, and the purpose of this protocol is to evaluate the efficacy and safety of TCM on SONFH based on the current studies.…”

Section: Introductionmentioning

confidence: 99%

Effectiveness and safety of traditional Chinese medicine in the treatment of steroid-osteonecrosis of femoral head

He¹,

Chen²,

Chen

et al. 2021

Medicine

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Background:Osteonecrosis of the femoral head (ONFH) is a common refractory disease in orthopedics. Overdose glucocorticoid application is a common trigger for ONFH. Traditional Chinese medicine (TCM), as a treatment for ONFH, has been shown to be effective in treating steroid-induced ONFH (SONFH). However, a systematic review and meta-analysis of them is lacking. We aim to systematically review the effectiveness and safety of TCM in the treatment of SONFH.Methods:We will search the following databases: PubMed, Embase, the Cochrane Library, MEDLINE, the Chinese Biomedical Literature Database, China Science and Technology Journal Database, China National Knowledge Infrastructure, and Wanfang Data (since the inception of the databases to the present). In addition, we will look for clinical trial registrations, prospective grey literature, relevant conference papers, and established study reference lists. We will use Review Manager 5.3 software for meta-analysis and heterogeneity assessment. We will evaluate the quality of the evidence using a hierarchy of recommendation assessment, development, and evaluation.Results:This study will systematically evaluate the efficacy and safety of TCM in the treatment of SONFH.Conclusion:This systematic review to evaluate the effectiveness and safety of TCM in the treatment of SONFH will provide updated evidence for clinical application.INPLASY registration number:INPLASY202170015.

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“…Indeed, HOTAIR also affects osteoblastogenesis. Yuan et al have shown that overexpression of HOTAIR inhibits osteogenic differentiation while promoting adipogenic differentiation of bone marrow stromal cells ( Yuan et al, 2020 ). Wei et al have found that HOTAIR inhibits osteogenic differentiation by suppressing miR-17-5p expression ( Wei et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Dysregulation of the HOTAIR-miR-152-CAMKIIα Axis in Craniosynostosis Results in Impaired Osteoclast Differentiation

Dong

Liu

et al. 2022

Front. Genet.

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Craniosynostosis is one of the most common craniofacial deformities demanding surgical treatment in infancy. LncRNA HOTAIR has verified its important role in osteogenesis and osteoarthritis. However, whether HOTAIR plays an essential role in the development of craniosynostosis is still unclear. In this study, we aimed to investigate the molecular role of HOTAIR in the osteoclast function and development of craniosynostosis.For osteoclast differentiation, RAW264.7 cells were induced by 50 ng/ml of RANKL and 10 ng/mL M-CSF, followed by TRAP staining. Cell proliferation and apoptosis were assayed by the CCK-8 kit and Annexin V-FITC apoptosis detection kit, respectively. The expression of HOTAIR was determined in PBMCs by qRT-PCR. Protein levels of all those involved genes were measured by Western blot assay. A luciferase reporter assay was used to determine the miRNA target validation. The HOTAIR expression in PBMCs from children with craniosynostosis was significantly downregulated. The results of cell proliferation and apoptosis assays indicated that silencing of HOTAIR could inhibit osteoclast differentiation and increase cell apoptosis. Moreover, the luciferase reporter assay revealed that the regulatory axis and HOTAIR-miR-152-CAMKIIα were the regulatory mechanisms of HOTAIR in the osteoclast function and development of craniosynostosis.In this study, our data showed that HOTAIR could promote osteoclast differentiation by binding miR-152. Furthermore, the HOTAIR/HOTAIR-miR-152-CAMKIIα axis was found to regulate osteoclast differentiation. These results indicate that the HOTAIR plays a crucial role in the development of osteoclasts.

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<p>Neohesperidin Ameliorates Steroid-Induced Osteonecrosis of the Femoral Head by Inhibiting the Histone Modification of lncRNA HOTAIR</p>

Cited by 15 publications

References 41 publications

Systemic Dietary Hesperidin Modulation of Osteoclastogenesis, Bone Homeostasis and Periodontal Disease in Mice

Systemic Dietary Hesperidin Modulation of Osteoclastogenesis, Bone Homeostasis and Periodontal Disease in Mice

Effectiveness and safety of traditional Chinese medicine in the treatment of steroid-osteonecrosis of femoral head

Dysregulation of the HOTAIR-miR-152-CAMKIIα Axis in Craniosynostosis Results in Impaired Osteoclast Differentiation

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