The Diagnosis of Hepatobiliary Diseases by Serum Enzyme Analyses

Konttinen, Aarne; Hupli, Veikko; Salmenkivi, K

doi:10.1111/j.0954-6820.1971.tb04417.x

Cited by 25 publications

References 30 publications

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Relationship between guanase activity in donor blood and the incidence of posttransfusional non-A, non-B hepatitis, and a possible method for preventing posttransfusional hepatitis

et al. 1986

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A total of 107 recipients, who did not show any evidence of hepatic disorders in pretransfusional liver function tests and gave a negative reaction for HBsAg, were observed from 3 weeks to 3 months after blood transfusion of 711 units of blood. The blood was judged suitable for use in transfusion because it had a normal level of ALT activity and gave a negative reaction for HBsAg. The guanase activities of the blood used for transfusion were examined. Cases in which an increase of ALT to at least twice the upper limit of normal persisted for at least 3 weeks and the ALT value increased to more than five times the normal upper limit at least once during this period, which also gave a negative reaction for HBsAg, were judged to have posttransfusional non-A, non-B hepatitis. Of 107 recipients, 18 developed posttransfusional non-A, non-B hepatitis. It was detected in 2 of 71 recipients (3%) with blood guanase activities below 3.5 units per liter and in 16 of 36 recipients (44%) with blood guanase activities above 3.6 units per liter. Thus, the incidence of posttransfusional non-A, non-B hepatitis was significantly higher in recipients with blood guanase activities above 3.6 units per liter. The incidence of posttransfusional non-A, non-B hepatitis increased linearly with increase in the level of guanase activity in donor blood. Thus, a high guanase activity in donor blood is considered to be an important predicting factor for posttransfusional non-A, non-B hepatitis.(ABSTRACT TRUNCATED AT 250 WORDS)

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Relationship between guanase activity in donor blood and the incidence of posttransfusional non-A, non-B hepatitis, and a possible method for preventing posttransfusional hepatitis

et al. 1986

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Differential Diagnosis of Liver Diseases by Enzyme Patterns. A Historical Review

Ritis

Cacciatore

1983

Clinical Hepatology

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Histochemical demonstration of guanase in human liver with guanine in bicine buffer as substrate

Ito

Keyser

et al. 1984

Histochem J

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Histochemical studies of human guanase (guanine deaminase) have seldom been undertaken, in part because of technical difficulties which result in heavy background staining. In this report, we describe a modified procedure in which the methodological inadequacies have been overcome. The modified technique has been applied to determine the intracellular and lobular distribution of guanase in normal human liver and in cases of primary biliary cirrhosis and alcoholic cirrhosis. Guanase was present within the cytoplasm of hepatocytes throughout the entire lobule. Enzyme activity was stronger on the sinusoidal side of the hepatocytes and in the periportal area. The reaction was weaker in perivenular hepatocytes. Portal components (bile ducts and veins), fibrous tissue and inflammatory cells were non-reactive, and the enzyme was absent from hepatocyte nuclei and membranes. Sections of skeletal muscle contained no guanase. The specificity of the reaction was confirmed by control tests on liver tissue and by the use of a specific inhibitor of guanase. It is concluded that the modified procedure overcomes the disadvantages inherent in the original method for guanase demonstration, allows the examination of fine cellular detail and should become a valuable histochemical tool with which to study diseases of the liver.

show abstract

The Diagnosis of Hepatobiliary Diseases by Serum Enzyme Analyses

Cited by 25 publications

References 30 publications

Relationship between guanase activity in donor blood and the incidence of posttransfusional non-A, non-B hepatitis, and a possible method for preventing posttransfusional hepatitis

Relationship between guanase activity in donor blood and the incidence of posttransfusional non-A, non-B hepatitis, and a possible method for preventing posttransfusional hepatitis

Differential Diagnosis of Liver Diseases by Enzyme Patterns. A Historical Review

Histochemical demonstration of guanase in human liver with guanine in bicine buffer as substrate

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